We performed Real-time PCR to detect the hsa-miR-197 expression levels in uterine leiomyomas tissues and paired normal myometrium separately；online databases including miRbase，UCSC，NCBI are employed to analysis the sequence conservation and genetic characteristics of hsa- miR- 197；miRNA databases such as miRanda，MirTarget2 and TargetScan are chosen to predict hsa- miR- 197 target genes；meanwhile，the intersection genes of these miRNA databases are chosen，and GO and Pathway analysis of these genes are carried out to explore the potential role of hsa-miR-197 playing in regulating the uterine leiomyomas occurrence and development. hsa-miR-197 functions in broad ground and participates in uterine leiomyomas multiple biology progress，which indicates that hsa- miR- 197 could regulate uterine leiomyomas occurrence and development.