Grephene oxide (GO) mixed matrix reverse osmosis (RO) membranes were prepared with metaphenylenediamine(MPD) and trimesoyl chloride (TMC) by interfacial polymerization. Themorphology of the membraneswere characterized by scanning electron microscopy(SEM). The separation performance and chlorine resistance were also investigated.The results shown that owing to the hydrophilicity of GO, the membranes appeared to be more hydrophilic and had higher pure water fluxes than the bared polyamide RO membrane,only with a slight change of rejection. When the content of GO added in the aqueous phase was 0.005wt%, the flux of the membrane increased by 20%. The mixed matrix RO membrane of polyamide and GO shownbetterchlorine resistance than PA RO membrane.