Resource Type

Journal Article 3

Year

2019 3

Keywords

SCNT 2

pig 2

PEDV 1

RNAi 1

TGEV 1

cloned 1

cloning 1

death 1

enucleation 1

piglet diarrhea 1

placenta 1

transgenic resistance breeding 1

umbilical cord 1

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNThave paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Effects of enucleation method on

Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 61-65 doi: 10.15302/J-FASE-2018227

Abstract:

Enucleation is a crucial procedure for mammalian somatic cell nuclear transfer (SCNT), especiallyeffects on the reprogramming potential of oocyte for cloning is beneficial for enhancing the outcome of SCNTaspiration-based (BA-B) enucleation and spindle imaging system-assisted (SIS-A) enucleation, and compared the pig SCNThigher oocyte enucleation success rate and tended to obtain a higher full term development rate of SCNTThese data indicate that SIS-A and BAPPS-B enucleation are better approaches for pig SCNT than BA-B enucleation

Keywords： cloning     enucleation     pig     SCNT    

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Comparison of birth weight and umbilical and placental characteristics of cloned and artificial insemination-derived piglets

Zheng AO, Chengfa ZHAO, Yanmin GAN, Xiao WU, Junsong SHI, Enqin ZHENG, Dewu LIU, Gengyuan CAI, Zhenfang WU, Zicong LI

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 54-60 doi: 10.15302/J-FASE-2018249

Abstract:

Somatic cell nuclear transfer (SCNT)-derived piglets have significantly higher stillbirth rate andThe question whether the low survival rate of SCNT piglets was related to birth weight, umbilical cordThe incidence of abnormal umbilical cord in SCNT and SCNT-liveborn (SCNT-LB) piglets was significantlyBirth weight, placental weight, placental surface area and placental efficiency in SCNT and SCNT-LB pigletsThus, the low survival rate of SCNT piglets may be associated with abnormal umbilical cord and placenta

Keywords： cloned     pig     death     placenta     SCNT     umbilical cord    

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Title Author Date Type Operation

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Effects of enucleation method on

Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI

Journal Article

Comparison of birth weight and umbilical and placental characteristics of cloned and artificial insemination-derived piglets

Zheng AO, Chengfa ZHAO, Yanmin GAN, Xiao WU, Junsong SHI, Enqin ZHENG, Dewu LIU, Gengyuan CAI, Zhenfang WU, Zicong LI

Journal Article