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2018 1

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

《化学科学与工程前沿（英文）》 2018年 第12卷 第2期   页码 311-314 doi: 10.1007/s11705-017-1697-0

摘要： A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

关键词： fragmentation     NCOU1     NTR     nitroreductase     nitrobenzyl-umbelliferone    

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

期刊论文