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2023 1

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气态递质 1

硫化氢 1

肥胖症 1

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硫化氢促进脂肪细胞分化、增生和肥大 Article

Richa Verma, 傅明, 杨广东, 吴凌云, 王睿

《工程（英文）》 2023年第20卷第1期页码 36-48 doi: 10.1016/j.eng.2022.09.010

摘要：

硫化氢（H₂S）在脂肪细胞和脂肪组织中内源性产生并刺激脂肪形成。然而，H₂S 对肥胖症发展的综合致病作用及其潜在机制尚不清楚。本研究发现降低的内源性H₂S水平降低了小鼠脂肪细胞中的脂质积累。在脂肪形成诱导6 d 后，外源性H₂S 处理显著增加了原代小鼠前脂肪细胞的脂肪生成。在脂肪生成的早
期阶段，H₂S增加细胞增殖并为细胞增生做好准备。H₂S处理10 d 后，前脂肪细胞的细胞表面积和直径明显增大，表明细胞肥大。虽然H₂S 刺激脂质积累和脂肪生成，但H₂S 对脂肪分解没有影响。随着营养过载和高葡萄糖/胰岛素孵化，H₂S 进一步刺激葡萄糖消耗并恶化脂肪细胞肥大。H₂S 上调增生基因[CCAAT/增强子结合蛋白（C/EBPβ）、细胞分裂周期25（Cdc25）、微染色体维持3（Mcm3）和细胞分裂周期（Cdc45）]和细胞周期蛋白依赖性激酶2 蛋白（Cdk2），调节细胞增殖。H₂S 还上调了胰岛素受体β（Irβ）激活的丝裂原活化蛋白激酶（MAPK）和蛋白激酶B（Akt）通路，从而导致脂肪生成。总之，H₂S 增加脂肪细胞分化、肥大和增生，提示它在肥胖症中起致病作用。

关键词：脂肪形成脂肪细胞气态递质葡萄糖硫化氢胰岛素脂质肥胖症

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Ablation of steroid receptor coactivator-3 in mice impairs adipogenesis and enhances energy expenditure

Ling-Yan XU PhD, Xin-Ran MA PhD, Xiao-Ying LI PhD, MD, Shu WANG PhD, Guang NING PhD, MD, Jie-Li LI PhD, Jian-Ming XU PhD,

《医学前沿（英文）》 2010年第4卷第2期页码 229-234 doi: 10.1007/s11684-010-0028-3

摘要： Obesity is a medical condition in which excess body fat has accumulated to an extent and may have an adverse effect on health, leading to reduced life expectancy, impaired energy homeostasis and increased health problems. The p160 steroid receptor coactivator (SRC) gene family members have been suggested to be involved in energy homeostasis, but the impact of SRC-3 ablation on white and brown adipose tissue needs to be elucidated. In the current study, we collected data and carried out morphological studies on the effect of SRC-3 deficiency on white adipose tissue (WAT) and brown adipose tissue (BAT). Primary cells were cultured to investigate the differentiation ability of both adipocytes. Western blot was applied to detect the expression of master genes governing adipogenesis and thermogenesis. We observed that SRC-3 mice were lean, with reduced WAT and decreased serum leptin levels, mainly due to the smaller white adipocyte size caused by impaired adipogenesis, presented by decreased peroxisome proliferator activated receptor (PPAR) expression. In the BAT, the lipid droplets decreased significantly in SRC-3 mice as demonstrated by histological analysis and electron microscopic observation, which could be explained by enhanced thermogenesis. The expression of thermogenic marker gene PPAR coactivator 1α and uncoupling protein-1 increased in BAT of SRC-3 mice, which proved our observations. Collectively, these results demonstrate that SRC-3 plays a key role in adipogenesis and energy expenditure.

关键词： steroid receptor coactivator-3 white adipose tissue brown adipose tissue obesity adipocytes energy expenditure