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3D细胞容器 1

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血管生成 1

非接触式多细胞共培养 1

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Visualization of vascular ultrastructure during osteogenesis by tissue engineering technique

ZHANG Kaigang, ZENG Bingfang, ZHANG Changqing

《医学前沿（英文）》 2007年第1卷第2期页码 181-184 doi: 10.1007/s11684-007-0034-2

摘要： The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique. We also studied the feasibility of improving tissue vascularization of th

关键词： feasibility engineering osteogenesis vascularization microscopy

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Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells

null

《医学前沿（英文）》 2016年第10卷第3期页码 286-296 doi: 10.1007/s11684-016-0456-9

摘要：

Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells (BMSCs) toward the injured area. This study aimed to determine the following: (1) the direction of rat BMSC (rBMSC) migration upon exposure to a direct current electric field (DCEF), (2) the optimal DCEF intensity and duration, and (3) the possible regulatory role of SDF-1/CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.

关键词： DCEF migration osteogenesis differentiation rBMSCs SDF-1/CXCR-4

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3D 打印细胞容器样支架在多细胞组织工程中的应用研究 Article

王晓亚, 张猛, 马景阁, 徐梦驰, 常江, Michael Gelinsky, 吴成铁

《工程（英文）》 2020年第6卷第11期页码 1276-1284 doi: 10.1016/j.eng.2020.08.001

摘要：

人工设计的非接触式多细胞共培养模型可以模仿人体组织的细胞内微环境，但其发展始终面临各种挑战。本研究利用3D打印技术，成功制得了一种含β-磷酸三钙/羟基磷灰石（β-TCP/HA）的细胞容器样支架，该支架设计了四种不同形状的微孔结构：三角形、正方形、平行四边形和长方形微孔结构。这些支架可以在非接触的方式下同时培养四种细胞。本研究构建了一种由人骨髓间质干细胞（HBMSC）、人脐静脉内皮细胞（HUVEC）、人脐静脉平滑肌细胞（HUVSMC）、人真皮成纤维细胞（HDF）组成的3D共培养模型，用以研究这些细胞在促进骨生成和血管生成过程中细胞的个体效应与协同效应。结果表明，相较于在3D细胞容器中仅培养一种细胞，共培养三种或四种细胞展现出了更高的细胞增殖率。HBMSC与HUVEC的细胞间相互作用的研究表明，含有四种独立空间结构的3D细胞容器可以通过放大共培养细胞的旁分泌效应促进细胞的骨生成和血管生成能力。此外，在3D细胞容器中建立多细胞非接触体系，特别是含有三种或四种细胞的共培养体系，相对于单细胞培养模式与两种细胞共培养模式来说，在促进细胞成骨分化和成血管分化方面展现出明显的优势。本研究为基于支架的多细胞非接触式共培养体系的发展提供了新的研究方向。

关键词： 3D细胞容器非接触式多细胞共培养相互作用血管生成骨生成