Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 3

年份

2023 1

2022 1

2008 1

关键词

circRNA 1

分子机制 1

敲低 1

非编码RNA 1

骨肉瘤 1

展开︾

检索范围：

排序：展示方式：

Expression of heat shock protein gp96 in osteosarcoma and its clinical significance

GUO Weichun, CHEN Anmin

《医学前沿（英文）》 2008年第2卷第2期页码 200-203 doi: 10.1007/s11684-008-0038-6

摘要： This study aims to detect the expression of heat shock protein gp96 (HSPgp96) in osteosarcoma and explore its role in anti-tumor immunity. Expression of HSPgp96 in 45 cases of osteosarcoma, including 24 cases of the osteoblast type, 21 cases of chondroblast type and 5 cases of normal bone tissue were detected by immunohistochemistry (IHC). The expression rate of HSPgp96 was 100% (45/45) in osteosarcoma, compared with 20% (1/5) in normal bone tissue. The average positive cell rates were 97.23% and 0.48% in osteosarcoma and normal bone tissue, respectively, and the difference between them was significant ( < 0.01). The positive cell rates in osteoblast type and chondroblast type were 97.78% and 96.56%, respectively, and the difference had no statistical significance ( > 0.05). The expression degree of HSPgp96 in osteosarcoma had a clear correlation with the pathologic degree ( < 0.05), while it had no relationship with the clinical stage ( > 0.05). The expression of HSPgp96 is high in osteosarcoma and it may be a significant index for judgment of malignancy degree and an important target site of anti-tumor immunity.

HTML PDF 收藏

Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance in osteosarcoma

《医学前沿（英文）》 2022年第16卷第6期页码 883-895 doi: 10.1007/s11684-022-0919-0

摘要： Metastasis and drug resistance are the leading causes of poor prognosis in patients with osteosarcoma. Identifying the relevant factors that drive metastasis and drug resistance is the key to improving the therapeutic outcome of osteosarcoma. Here, we reported that autophagy was highly activated in metastatic osteosarcoma. We found increased autophagolysosomes in metastatic osteosarcoma cell lines by using electron microscopy, Western blot, and immunofluorescence experiments. We further examined the expression of the autophagy-related genes Beclin1 and LC3B in 82 patients through immunohistochemistry and found that Beclin1 and LC3B were highly related to unfavorable prognosis of osteosarcoma. Knockdown of Beclin1 and LC3B reduced invasion, metastasis, and proliferation in metastatic osteosarcoma cells. In vitro and in vivo studies also demonstrated that inhibiting by 3-MA inhibited cell growth and metastasis. Moreover, we demonstrated that autophagy-related genes were activated by SEs and that the inhibition of SEs by JQ-1 decreased the metastasis of osteosarcoma. Overall, our findings highlighted the association of autophagy with osteosarcoma progression and shed new light on autophagy-targeting therapy for osteosarcoma.

关键词： osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B

HTML PDF 收藏

敲低特异性环状非编码RNA显著抑制骨肉瘤的进展 Article

王世东, 张红亮, 李博, 陈成龙, 任婷婷, 黄怡, 刘凯, 李敬敬, 郭卫

《工程（英文）》 2023年第21卷第2期页码 187-193 doi: 10.1016/j.eng.2021.12.007

摘要：

骨肉瘤是一种间充质组织来源的恶性肿瘤，好发于儿童和青少年，发生肺转移常导致患者死亡。关于骨肉瘤进展的机制仍不清楚。因此，迫切需要开发新的骨肉瘤治疗靶点和治疗模式。异常表达的非编码环状RNA（circRNA）对骨肉瘤的发生和发展至关重要。本研究的目的是探索一种新的circRNA circ_000203 在骨肉瘤中的表达和作用，阐明其潜在机制。我们发现，在骨肉瘤细胞系和组织中circ_000203 高表达，并且circ_000203 敲低显著抑制了体外和体内的骨肉瘤进展。此外，我们发现circ_000203 是miR-26b-5p 的海绵，而miR-26b-5p 是骨形态发生蛋白受体2（BMPR2）的上游调节因子。因此，BMPR2 的过表达可以减轻对骨肉瘤进展的抑制作用。这表明，敲低circ_000203 可通过miRNA介导的BMPR2 下调抑制骨肉瘤的进展。我们的发现为理解骨肉瘤的发生和发展提供了重要的见解。

关键词：非编码RNA 骨肉瘤 circRNA 分子机制敲低