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Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition

null

《医学前沿（英文）》 2016年 第10卷 第3期   页码 311-319 doi: 10.1007/s11684-016-0460-0

摘要：

We evaluated the roles of calpain cleavage-related mutations of the integrin β3 cytoplasmic tail in integrin αIIbβ3 bidirectional signaling using a trans-dominant inhibition model. Chimeric Tac-β3 proteins (i.e., Tac-β3, Tac-β3D741, Tac-β3D747, Tac-β3D754, Tac-β3D759, and Tac-β3DNITY) consisting of the extracellular and transmembrane domains of human IL-2 receptor (Tac) and the human integrin β3 cytoplasmic domain were stably expressed in the 123 CHO cells harboring human glycoprotein Ib-IX and wild-type integrin αIIbβ3. The different cells were assayed for stable adhesion and spreading on immobilized fibrinogen, and for binding soluble fibrinogen representing outside-in and inside-out signaling events, respectively. The chimeric protein Tac-β3 inhibited, and Tac-β3DNITY partially attenuated stable adhesion and spreading. Tac-β3, Tac-β3D759, Tac-β3DNITY, and Tac-β3D754, but not Tac-β3D747 or Tac-β3D741, impaired the soluble fibrinogen binding. Results indicated that the bidirectional signaling was significantly inhibited by Tac-β3 and Tac-β3DNITY, albeit to a much lesser extent. Moreover, only inside-out signaling was impaired in the 123/Tac-β3D759 and 123/Tac-β3D754 cells in contrast to an intact bidirectional signaling in the 123/Tac-β3D747 and 123/Tac-β3D741 cells. In conclusion, the calpain cleavage of integrin β3 resulted in the regulatory effects on signaling by interrupting its interaction with cytoplasmic proteins rather than altering its conformation, and may thus regulate platelet function.

关键词： integrin β3     signal transduction     trans-dominant inhibition model    

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Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

《环境科学与工程前沿（英文）》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0896-8

摘要： Valerate-hydrolysate enriched culture showed great ability to produce 3HV and 3H2MV. Valerate-hydrolysate enriched culture had more in the community. Mixed iso-/n-valerate was effective at balancing microbial growth and PHAs synthesis. Co-substrates of valerate and propionate raised the fraction of 3HV and 3H2MV. The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HV and 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

关键词： Polyhydroxyalkanoates (PHAs)     Valerate     Mixed culture     3-hydroxyvalerate (3HV)     Propionate    

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Heavy metal accumulation and phytostabilization potential of dominant plant species growing on manganese

YANG Shengxiang,LIANG Shichu,YI Langbo,XU Bibo,CAO Jianbing,GUO Yifeng,ZHOU Yu

《环境科学与工程前沿（英文）》 2014年 第8卷 第3期   页码 394-404 doi: 10.1007/s11783-013-0602-4

摘要： Screening plants that are hypertolerant to and excluders of certain heavy metals plays a fundamental role in a remediation strategy for metalliferous mine tailings. A field survey of terrestrial higher plants growing on Mn mine tailings at Huayuan, Hunan Province, China was conducted to identify candidate species for application in phytostabilization of the tailings in this region. In total, 51 species belonging to 21 families were recorded and the 12 dominant plants were investigated for their potential in phytostabilization of heavy metals. Eight plant species, , , , , , , , and accumulated much lower concentrations of heavy metals in shoots and roots than the associated soils and bioconcentration factors (BFs) for Cd, Mn, Pb and Zn were all<1, demonstrating a high tolerance to heavy metals and poor metals translocation ability. The field investigation also found that these species grew fast, accumulated biomass rapidly and developed a vegetation cover in a relatively short time. Therefore, they are good candidates for phytostabilization purposes and could be used as pioneer species in phytoremediation of Mn mine tailings in this region of South China.

关键词： Mn mine tailings     heavy metal accumulation     phytostabilization    

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Hydrogen production by catalytic gasification of cellulose in supercritical water

GUAN Yu, PEI Aixia, GUO Liejin

《化学科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 176-180 doi: 10.1007/s11705-008-0026-z

摘要： Cellulose, one of the important components of biomass, was gasified in supercritical water to produce hydrogen-rich gas in an autoclave which was operated batch-wise under high-pressure. KCO and Ca(OH) were selected as the catalysts (or promoters). The temperature was kept between 450°C and 500°C while pressure was maintained at 24–26 MPa. The reaction time was 20 min. Experimental results showed that the two catalysts had good catalytic effect and optimum amounts were observed for each catalyst. When 0.2 g KCO was added, the hydrogen yield could reach 9.456 molkg which was two times of the H amount produced without catalyst. When 1.6 g Ca(OH) was added, the H yield was 8.265 molkg which is lower than that obtained using KCO as catalyst but is still 1.7 times that achieved without catalyst. Comparing with the results obtained using KCO or Ca(OH) alone, the use of a combination of KCO and Ca(OH) could increase the H yield by up to 2.5 times that without catalyst and 25% and 45% more than that obtained using KCO and Ca(OH) alone, respectively. It was found that methane was the dominant product at relatively low temperature. When the temperature was increased, the methane reacts with water and is converted to hydrogen and carbon dioxide.

关键词： reaction     temperature     combination     catalytic effect     dominant product    

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Simulation of cross-flow-induced vibration of tube bundle by surface vorticity method

WANG Fenghao, JIANG Gedong, Lin John Zhang

《能源前沿（英文）》 2008年 第2卷 第3期   页码 243-248 doi: 10.1007/s11708-008-0049-7

摘要： A fluid-structure interaction model based on Surface Vorticity Method (SVM) was used to study flow-induced vibrations of tube bundles in medium space ratio. The flow-induced vibrations of four tubes in a rotated square and a staggered tube bundle in three-row and five-column arrangements were simulated in the high sub-critical Reynolds number () range. The results on fluid forces, tube responses and vorticity maps were presented. The vorticity maps of the four rotated-square tubes changed dramatically when the rigid tubes were replaced by the flexible tubes. From the vorticity maps and vibration responses of the staggered tube bundle of different structural parameters, it was found that with the decrease of tube natural frequency, the maximal vibration response moved from the third row to the first. The results also showed that when more flexible tubes are used, the flow pattern changed drastically and the fluid-structure interaction imposed a dominant impact on the flow.

关键词： dominant     sub-critical Reynolds     staggered     Vorticity     three-row    

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Stability of an annular viscous liquid jet in compressible gases with different properties inside and outside of the jet

Chunji YAN, Maozhao XIE,

《能源前沿（英文）》 2010年 第4卷 第2期   页码 198-204 doi: 10.1007/s11708-009-0054-5

摘要： A spatial linear instability analysis is conducted on an annular viscous liquid jet injected into compressible gases and a three-dimensional model of the jet is developed. The model takes into account differences between the velocities, densities of the gases inside and outside of the liquid jet. Theoretical analysis reveals that there exist 9 dimensionless parameters controlling the instability of the liquid jet. Numerical computations reveal some basic characteristics in the breakup and atomization process of the liquid jet as well as influences of these relevant parameters. Major observations and findings of this study are as follows. The Mach number plays a destabilizing role and the inner Mach number has a greater effect on the jet instability than the outer Mach number. The Reynolds number always tends to promote the instabilities of the liquid jet, but its influence is very limited. The Weber number and the gas-to-liquid density ratio also have unstable effects and can improve the atomization of liquid jets. Furthermore, the effects of the Weber number and gas-to-liquid density ratio on the maximum growth rates of axisymmetric and non-axisymmetric disturbances and corresponding dominant wave numbers are manifested in a linear way, while that of the Mach number is non-linear. The effect of Reynolds on the maximum growth rates is non-linear, but the dominant wavenumber is almost not affected by the Reynolds number.

关键词： liquid jet     dominant wave number     compressibility     instability    

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Numerical simulation of laminar premixed combustion in a porous burner

ZHAO Pinghui, CHEN Yiliang, LIU Minghou, DING Min, ZHANG Genxuan

《能源前沿（英文）》 2007年 第1卷 第2期   页码 233-238 doi: 10.1007/s11708-007-0032-8

摘要： Premixed combustion in porous media differs substantially from combustion in free space. The interphase heat transfer between a gas mixture and a porous medium becomes dominant in the premixed combustion process. In this paper, the premixed combustion of CH

关键词： interphase     mixture     dominant     Premixed combustion     premixed combustion    

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Dynamic modelling and simulation of a post-combustion CO capture process for coal-fired power plants

《化学科学与工程前沿（英文）》 2022年 第16卷 第2期   页码 198-209 doi: 10.1007/s11705-021-2057-7

摘要： Solvent-based post-combustion capture technologies have great potential for CO2 mitigation in traditional coal-fired power plants. Modelling and simulation provide a low-cost opportunity to evaluate performances and guide flexible operation. Composed by a series of partial differential equations, first-principle post-combustion capture models are computationally expensive, which limits their use in real time process simulation and control. In this study, we propose a first-principle approach to develop the basic structure of a reduced-order model and then the dominant factor is used to fit properties and simplify the chemical and physical process, based on which a universal and hybrid post-combustion capture model is established. Model output at steady state and trend at dynamic state are validated using experimental data obtained from the literature. Then, impacts of liquid-to-gas ratio, reboiler power, desorber pressure, tower height and their combination on the absorption and desorption effects are analyzed. Results indicate that tower height should be designed in conjunction with the flue gas flow, and the gas-liquid ratio can be optimized to reduce the reboiler power under a certain capture target.

关键词： CO2 capture     post-combustion capture     simulation     dominant factor    

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Mutation analysis of KCNQ1, KCNH2, SCN5A, KCNE1 and KCNE2 genes in Chinese patients with long QT syndrome

DU Rong, TIAN Li, YUAN Guohui, LI Jin, REN Faxin, GUI Le, LI Wei, ZHANG Shouyan, KANG Cailian, YANG Junguo

《医学前沿（英文）》 2007年 第1卷 第3期   页码 312-315 doi: 10.1007/s11684-007-0060-0

摘要： Long QT syndrome (LQTS) is the prototype of the cardiac ion channelopathies, which cause syncope and sudden death. Inherited LQTS is represented by the autosomal dominant Romano-ward syndrome (RWS), which is not accompanied by congenital deafness, and the autosomal recessive Jervell and Lange-Nielsen syndrome (JLNS), which is accompanied by congenital deafness. The LQTS-causing mutations have been reported in patients and families from Europe, North America and Japan. Few genetic studies have been carried out in families with JLNS from China. This study investigates the molecular pathology in four families with LQTS (including a family with JLNS) in the Chinese population. Polymerase chain reaction and DNA sequencing were used to screen for , , , and mutation. A missense mutation G314S in an RWS family was identified, and a single nucleotide polymorphism (SNP) G643S was indentified in the of the JLNS family. In this JLNS family, another heterozygous novel mutation in exon 2a was found in of the patients. Our data provide useful information for the identification of polymorphisms and mutations related to LQTS and the Brugada Syndrome (BS) in Chinese populations.

关键词： recessive Jervell     LQTS-causing     population     autosomal dominant     syndrome    

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标题 作者 时间 类型 操作

Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition

null

期刊论文

Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

期刊论文

Heavy metal accumulation and phytostabilization potential of dominant plant species growing on manganese

YANG Shengxiang,LIANG Shichu,YI Langbo,XU Bibo,CAO Jianbing,GUO Yifeng,ZHOU Yu

期刊论文

Hydrogen production by catalytic gasification of cellulose in supercritical water

GUAN Yu, PEI Aixia, GUO Liejin

期刊论文

Simulation of cross-flow-induced vibration of tube bundle by surface vorticity method

WANG Fenghao, JIANG Gedong, Lin John Zhang

期刊论文

Stability of an annular viscous liquid jet in compressible gases with different properties inside and outside of the jet

Chunji YAN, Maozhao XIE,

期刊论文

Numerical simulation of laminar premixed combustion in a porous burner

ZHAO Pinghui, CHEN Yiliang, LIU Minghou, DING Min, ZHANG Genxuan

期刊论文

Dynamic modelling and simulation of a post-combustion CO capture process for coal-fired power plants

期刊论文

Mutation analysis of KCNQ1, KCNH2, SCN5A, KCNE1 and KCNE2 genes in Chinese patients with long QT syndrome

DU Rong, TIAN Li, YUAN Guohui, LI Jin, REN Faxin, GUI Le, LI Wei, ZHANG Shouyan, KANG Cailian, YANG Junguo

期刊论文