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DsbA-DsbA

Ye Liu,Wenyong Zhang,Xubin Yang,Guangbo Kang,Damei Wang,He Huang

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 4,   Pages 511-521 doi: 10.1007/s11705-015-1519-1

Abstract: A co-expressing system of DsbA-DsbA was suggested for the first time to enhance the soluble expressionVectors pET39b-trypsin and pET28a-DsbA-DsbA -trypsin with the above two DsbA fusion tag were constructedWhile in BL21 (DE3) strain with vector pET28a-DsbA-DsbA -trypsin, the soluble expression of trypsinThe results suggested that (1) besides the primary function in periplasm, leaderless DsbA or DsbA mayprotein DsbA-DsbA -trypsin.

Keywords： DsbA     DsbA-DsbAmut     soluble expression     trypsin     chaperon    

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DsbA-DsbA

Ye Liu,Wenyong Zhang,Xubin Yang,Guangbo Kang,Damei Wang,He Huang

Journal Article