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Journal Article 1

Year

2018 1

Keywords

NCOU1 1

NTR 1

fragmentation 1

nitrobenzyl-umbelliferone 1

nitroreductase 1

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 2,   Pages 311-314 doi: 10.1007/s11705-017-1697-0

Abstract: A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) triggerfluorophore umbelliferone an NTR-catalyzed reduction of the nitro group.In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at455 nm and was selective for NTR over other reductive enzymes.These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

Keywords： fragmentation     NCOU1     NTR     nitroreductase     nitrobenzyl-umbelliferone    

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

Journal Article