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Rational Design, Over-expression and Characterization of a Novel Staphylokinase with Lower Tendency of Polymerization

Song Gang,Yu Min,Mo Wei,Song Houyan

Strategic Study of CAE 2000, Volume 2, Issue 11, Pages 68-72

Abstract: On the basis of crystal structure of staphylokinase, the dimer structure was modeled by software GRAMMThe computer model of staphylokinase dimer provides a template for the design of improved staphylokinase

Keywords： staphylokinase dimer molecular docking molecular design site-directed mutagenesis

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Diporphyrin tweezer for multichannel spectroscopic analysis of enantiomeric excess

Daniel T. Payne, Mandeep K. Chahal, Václav Březina, Whitney A. Webre, Katsuhiko Ariga, Francis D’Souza, Jan Labuta, Jonathan P. Hill

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1, Pages 28-40 doi: 10.1007/s11705-019-1869-1

Abstract: Chiral 1,1’-binaphthyl-linked diporphyrin ‘tweezers’ ( )- /( )- and the corresponding zinc(II) complexes ( )- /( )- were prepared as chiral host molecules, and their utility for chiral analyses (especially enantiomeric excess ( ) determinations) were evaluated. Tris(1- -dodecyl)porphyrins were used for the first time as the interacting units. Host capabilities of the diporphyrin tweezers were investigated by titrations with ( , )- and ( , )-cyclohexane-1,2-diamine (CHDA). The host molecules could be used as multichannel probes of by using UV-vis, circular dichroism (CD), fluorescence emission and H nuclear magnetic resonance ( H-NMR) methods. Chiral configurations could also be differentiated using CD or H-NMR spectroscopy. All three optical techniques give good resolution of with reasonable sensitivity considering the low concentrations used (ca. 10 mol·L ). The determination of CHDA enantiomers using NMR spectroscopy is also possible because of the reasonably well separated resonances in the case of ( , )- and ( , )-CHDA. Non-metallated ( )- /( )- hosts could not be used to detect chiral information in a strongly acidic chiral guest. This work demonstrates the utility of 1,1’-binapthyl-linked chiral hosts for chiral analysis of ditopically interacting enantiomers.

Keywords： porphyrin dimer chirality enantiomeric excess CD fluorescence