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Gear fault diagnosis using gear meshing stiffness identified by gearbox housing vibration signals
Frontiers of Mechanical Engineering 2022, Volume 17, Issue 4, doi: 10.1007/s11465-022-0713-3
Keywords: gearbox fault diagnosis meshing stiffness identification transfer path signal processing
QIANG Zhimin, BEN Weiwei, HUANG Chin-Pao
Frontiers of Environmental Science & Engineering 2008, Volume 2, Issue 4, Pages 397-409 doi: 10.1007/s11783-008-0074-0
Keywords: identified 1 1-dichloroethylene kinetic Dichloroacetic Chloride
A new opsonin-like protein identified from coelomic fluid of sea cucumber Apostichopus japonicus
Mai Kangsen,Meng Fanyi,Ma Hongming,Zhang Wenbing
Strategic Study of CAE 2009, Volume 11, Issue 10, Pages 108-114
In order to determine if opsonin-like molecules exist in the coelomic fluid of sea cucumber Apostichopus japonicus and identify their properties, immunochemical methods including phagocytosis assay, SDS-PAGE, gel filtration and mass spectrometric analysis were performed. The phagocytosis of sea cucumber phagocytes was quantified in vitro using heat-killed yeast cells as target cells. Phagocytes of the sea cucumber were capable of phagocytosis in isotonic buffer, cell-free coelomic fluid (CFCF) and yeast cell-adsorbed CFCF, but the phagocytosis was significantly enhanced when the incubation medium was CFCF. This phagocytosis promoting activity of CFCF was eliminated by pre-adsorption of CFCF with yeast cells. SDS-PAGE analysis showed that a protein with 18 kDa was able to bind to yeast cells. By using gel filtration, the protein was isolated from coelomic fluid of the sea cucumber. Functional test of this molecule demonstrated that it was a phagocytosis-enhancing opsonin-like molecule. However, the phagocytosis-enhancing function of the opsonin-like molecule was dependent on some unknown factor(s) in the CFCF, which needs further investigations. Mass spectrometric analysis indicated that this opsonin-like molecule is a new protein.
Keywords: postichopus japonicus phagocyte coelomic fluid opsonin-like molecule
Title Author Date Type Operation
Gear fault diagnosis using gear meshing stiffness identified by gearbox housing vibration signals
Journal Article
Fenton process for degradation of selected chlorinated aliphatic hydrocarbons exemplified by trichloroethylene, 1,1-dichloroethylene and chloroform
QIANG Zhimin, BEN Weiwei, HUANG Chin-Pao
Journal Article