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transfection 2

transfection efficiency 2

α-helical polypeptide 1

ECs 1

HindIII 1

PP2A activity 1

cellular regulation 1

cytoplasm 1

detection 1

different 1

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: and specific repression of human heat shock protein 90β (Hsp90β) gene expression and to compare the transfectionefficiency of the plasmids in varying conditions of transfection.The transfection efficiency was measured by detection of enhanced green fluorescence protein (EGFP).After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293Transfection efficiency was still low in the HepG2 cells.

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 35-38 doi: 10.1007/s11684-008-0007-0

Abstract: this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfectionAfter podocin transfection, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells

Keywords： pEGFP-NPHS2 expression     transfection     pEGFP-C2     cytoplasm     digested    

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Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3

WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 366-369 doi: 10.1007/s11684-008-0070-6

Abstract: The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and functionFoxp3-gene transfected CD4 T cells could express Foxp3 and transfection of Foxp3 gene up-regulated theAfter transfection, the proliferation of CD4 T cells was eliminated.
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Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral

Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 415-420 doi: 10.1007/s11684-009-0072-z

Abstract: The aim of this study was to investigate the inhibitory effects of recombinant adenovirus vector carrying tissue inhibitor of metalloproteinase-3 (RAdTIMP-3) against degeneration of rabbit intervertebral disc. Thirty Japanese white rabbits of 4 months old were randomly divided into 5 groups. Mild or moderate rabbit lumbar disc degeneration model was constructed with the controllable axial loading device by imposing 98N pressure at the discs for 2 weeks. Various doses of virus were injected into the degenerated discs as follows: 20μL of normal saline in group 1; 20μL of RAd66 (an empty adenovirus vector, 1.0×10OPU/mL) in group 2; and 20, 10, and 5μL of RAdTIMP-3 (1.0×10OPU/mL) in groups 3, 4, and 5, respectively. Two weeks after the injection, the discs were collected for investigations, including assessment of degeneration degrees according to the Thompson’s grading system, reverse-transcription polymerase chain reaction (RT-PCR) assay for TIMP-3 gene, Safranin O-Fast green staining, and immunohistochemical staining for TIMP-3 and type II collagen. According to Thompson’s criteria, the degeneration of groups 3, 4, and 5, especially group 3, was alleviated as compared with groups 1 and 2. RT-PCR revealed that the expression of TIMP-3 in groups 3, 4, and 5, especially in group 3, was significantly enhanced as compared with group 1 (<0.01). Both Safranin O-Fast green staining and type II collagen staining demonstrated better reserved integrity of disc matrix in groups 3, 4, and 5 than in groups 1 and 2. TIMP-3 staining exhibited an obvious increase of positive-staining rate in groups 3, 4, and 5 as compared with group 1. The positive-staining rate in group 3 (79.42%±1.35%) was about 3times that of group 1 (25.47%±5.46%, <0.01). RAdTIMP-3 can effectively protect the matrix of rabbit intervertebral disc against overloading-induced degeneration in a dose-dependent manner, resulting in the alleviation of disc degeneration.

Keywords： tissue inhibitor of metalloproteinase-3     intervertebral disc     rabbit     gene therapy    

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Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 521-528 doi: 10.1007/s11705-017-1612-8

Abstract: The PPABLG was able to condense DNA to form nanocomplexes, and they exhibited significantly improved transfectionefficiencies compared with commercial transfection reagent Lipofectamine 2000 and classical cell penetrating

Keywords： α-helical polypeptide     plant gene delivery     protoplast     intact leaves     transfection    

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Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5,   Pages 889-901 doi: 10.1007/s11705-020-1919-8

Abstract: PEI-PEG-REDV-G-TAT-G-NLS copolymers with the aim to develop the gene carriers with low cytotoxicity and high transfectionThe results of transfection demonstrated that these multitargeting gene complexes possessed relativelyhigh transfection efficiency.REDV-G-TAT-G-NLS peptide, the multitargeting polycationic gene carrier with low cytotoxicity and high transfection

Keywords： gene carriers     multitargeting function     ECs     transfection efficiency    

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Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 235-238 doi: 10.1007/s11684-008-0044-8

Abstract: tau at different sites, as well as PP2A activity were detected at different time points after siRNA transfectionTwelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreased

Keywords： hyperphosphorylation     PP2A activity     cellular regulation     siRNA     siRNA transfection    

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Title Author Date Type Operation

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

Journal Article

Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3

WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng

Journal Article

Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral

Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,

Journal Article

Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Journal Article

Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Journal Article

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Journal Article