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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Engineering 2018, Volume 4, Issue 6,   Pages 888-893 doi: 10.1016/j.eng.2018.09.007

Abstract:

Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis exhibits a strong abilityTherefore, we explored the interaction between PETase and the substrate (a dimer of the PET monomer ethyleneterephthalate, 2PET), using a model of PETase and its substrate.These PETase mutants were designed and tested.Thus, this study has created enhanced artificial PETase enzymes through the rational protein engineering

Keywords： Polyesterase     PET degradation     Cell-free protein synthesis     Polyester     PETase    

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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Journal Article