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Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 66-72 doi: 10.15302/J-FASE-2018229
Keywords: piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding
Effects of enucleation method on
Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 61-65 doi: 10.15302/J-FASE-2018227
Enucleation is a crucial procedure for mammalian somatic cell nuclear transfer (SCNT), especiallyeffects on the reprogramming potential of oocyte for cloning is beneficial for enhancing the outcome of SCNTaspiration-based (BA-B) enucleation and spindle imaging system-assisted (SIS-A) enucleation, and compared the pig SCNThigher oocyte enucleation success rate and tended to obtain a higher full term development rate of SCNTThese data indicate that SIS-A and BAPPS-B enucleation are better approaches for pig SCNT than BA-B enucleation
Keywords: cloning enucleation pig SCNT
Zheng AO, Chengfa ZHAO, Yanmin GAN, Xiao WU, Junsong SHI, Enqin ZHENG, Dewu LIU, Gengyuan CAI, Zhenfang WU, Zicong LI
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 54-60 doi: 10.15302/J-FASE-2018249
Somatic cell nuclear transfer (SCNT)-derived piglets have significantly higher stillbirth rate andThe question whether the low survival rate of SCNT piglets was related to birth weight, umbilical cordThe incidence of abnormal umbilical cord in SCNT and SCNT-liveborn (SCNT-LB) piglets was significantlyBirth weight, placental weight, placental surface area and placental efficiency in SCNT and SCNT-LB pigletsThus, the low survival rate of SCNT piglets may be associated with abnormal umbilical cord and placenta
Title Author Date Type Operation
Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
Journal Article
Effects of enucleation method on
Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI
Journal Article