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enucleation 2

Intracellular damage 1

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Oocyte enucleation 1

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cloning 1

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Effects of enucleation method on

Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 61-65 doi: 10.15302/J-FASE-2018227

Abstract: Using an oocyte enucleation technique that can obtain a high enucleation rate but has minimal negativewith BA-B enucleation and blind aspiration plus post-enucleation staining-based (BAPPS-B) enucleationSIS-A enucleation achieved a significantly higher oocyte enucleation success rate and tended to obtaincloned porcine embryos than BA-B enucleation.These data indicate that SIS-A and BAPPS-B enucleation are better approaches for pig SCNT than BA-B enucleation

Keywords： cloning enucleation pig SCNT

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Mouse Oocyte Enucleation with Surface Tension Assisted Method

Meng Qinggang,Zhu Shien,Zeng Shenming,Zhang Zhongcheng

Strategic Study of CAE 2001, Volume 3, Issue 11, Pages 65-69

Abstract:

In the present experiment a new simple method-Surface Tension Aided (STA)- for mouse oocyte enucleation

Keywords： surface tension assisted enucleation oocyte mouse

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Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning Article

Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao

Engineering 2023, Volume 24, Issue 5, Pages 73-83 doi: 10.1016/j.eng.2022.04.016

Abstract: In this study, we proposed an intracellular strain evaluation-based oocyte enucleation method to reducethe intracellular velocity field and then used the intracellular strain as a criterion to improve the enucleationIn addition to the cloning technique, the intracellular strain evaluation-based enucleation method is

Keywords： Oocyte enucleation Robotic cloning Intracellular strain Intracellular damage Optical flow