Search scope:
排序： Display mode:
Wei Zhang,Xiaoli Yang,Jingjing Song,Xin Zheng,Jianbo Chen,Panpan Ma,Bangzhi Zhang,Rui Wang
Engineering 2015, Volume 1, Issue 4, Pages 500-505 doi: 10.15302/J-ENG-2015106
The emergence of multidrug-resistant bacteria creates an urgent need for alternative antibiotics with new mechanisms of action. In this study, we synthesized a novel type of antimicrobial agent, Acr3-NLS, by conjugating hydrophobic acridines to the N-terminus of a nuclear localization sequence (NLS), a short cationic peptide. To further improve the antimicrobial activity of our agent, dimeric (Acr3-NLS)2 was simultaneously synthesized by joining two monomeric Acr3-NLS together via a disulfide linker. Our results show that Acr3-NLS and especially (Acr3-NLS)2 display significant antimicrobial activity against gram-negative and gram-positive bacteria compared to that of the NLS. Subsequently, the results derived from the study on the mechanism of action demonstrate that Acr3-NLS and (Acr3-NLS)2 can kill bacteria by membrane disruption and DNA binding. The double targets–cell membrane and intracellular DNA–will reduce the risk of bacteria developing resistance to Acr3-NLS and (Acr3-NLS)2. Overall, this study provides a novel strategy to design highly effective antimicrobial agents with a dual mode of action for infection treatment.
Keywords： acridine nuclear localization sequence conjugate antimicrobial activity mechanism of action
Title Author Date Type Operation
京公网安备 11010502051620号
