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Wang Guixing,Zhu Yimei,Hou Jilun,Zhang Xiaoyan,Wang Yufen,Sun Zhaohui,Jiang Xiufeng,Liu Haijin
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 33-41
We studied the gonadogenesis, gender differentiation and early stage development of doubled haploid Japanese flounder (Paralichthys olivaceus) by using histological sections. The results indicated that there were some female, male and also undifferentiated individuals in the doubled haploid population, and the ratio was 56%: 38%: 6%. The gender differentiation in female doubled haploid was later than the normal one. At 100 days, the ovarian cavity was still not closed; and at 110 days, there were a large number of transitional cells from oogonium to oocyte on the spawning board, and the stageⅠoocyte was not observed. For the male double haploid, the gender differentiation was basically the same in time with the normal one. With the development of gonad, there were some abnormal oocytes in the ovaries of double haploids, and some ovaries degenerated. There were also some degeneration appeared in the testes of male doubled haploids. At 9-11 months old, the testis of doubled haploid was at stage Ⅲ, while the testis of normal Japanese flounder develop into stage Ⅳ. From 11 months, the testes of some doubled haploid begun to degenerate. At 13-15 months old, there were only spermatogonium in the seminiferous lobules, and the primary spermatocyte disappeared, like the testis in stageⅡ.
Keywords： Japanese flounder Paralichthys olivaceus doubled haploid gonad degeneration.
Hou Jilun,Li Chao,Wang Guixing,Zhang Xiaoyan,Sun Zhaohui,Wang Yufen,Jiang Xiufeng,Liu Haijin
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 26-32
Three third-generation of successive meiogynogenetic families (Meio-G3-1, Meio-G3-2 and Meio-G3-3) in Japanese flounder (Paralichthys olivaceus) were induced by artificially meiogynogenesis, which the eggs were activated by UV-irradiated red sea bream (Pagrus major) sperm, and followed with the cold-shock treatment. We evaluated the genetic structure of these three families using 30 microsatellite markers, and the efficiency of successive meiogynogenesis for producing highly inbred line was quantified by calculating the homozygosity and genetic similarity. The numbers of alleles detected were 35, 38 and 37 in Meio-G3-1, Meio-G3-2 and Meio-G3-3, respectively. And for these three families studied, the average numbers of alleles (A) were 1.17, 1.27 and 1.23; the average observed heterozygosity (Ho) were 0.1467, 0.2556 and 0.2056; the average homozygoisty among the 30 analyzed loci were 0.8533, 0.7444, and 0.7944. For the genetic similarity, the average similarity index between offspring within family was 0.9913, 0.9918 and 0.9838; between dam and offspring was 0.996, 0.9968 and 0.9923; and between families was 0.9717 (Meio-G3-1 and Meio-G3-2), 0.9810 (Meio-G3-1 and Meio-G3-3) and 0.9714 (Meio-G3-2 and Meio-G3-3), respectively. Our results indicate that the artificially induced successive meiogynogenesis can increase the homozygosity of individual, and also the genetic similarity of offspring within family and between families. And therefore, the artificially induced successive meiogynogenesis is a good method to establish inbred lines in fish.
Keywords： Japanese flounder Paralichthys olivaceus successive meiogynogenesis homozygosity inbred line
Qian Zhou, Ya-dong Chen, Sheng Lu, Yang Liu, Wen-teng Xu, Yang-zhen Li, Lei Wang, Na Wang, Ying-ming Yang, Song-lin Chen
Engineering 2021, Volume 7, Issue 3, Pages 406-411 doi: 10.1016/j.eng.2020.06.017
Single nucleotide polymorphism (SNP) arrays are a powerful genotyping tool used in genetic research and genomic breeding programs. Japanese flounder (Paralichthys olivaceus) is an economically-important aquaculture flatfish in many countries. However, the lack of high-efficient genotyping tools has impeded the genomic breeding programs for Japanese flounder. We developed a 50K Japanese flounder SNP array, "Yuxin No. 1," and report its utility in genomic selection (GS) for disease resistance to bacterial pathogens. We screened more than 42.2 million SNPs from the whole-genome resequencing data of 1099 individuals and selected 48 697 SNPs that were evenly distributed across the genome to anchor the array with Affymetrix Axiom genotyping technology. Evaluation of the array performance with 168 fish showed that 74.7% of the loci were successfully genotyped with high call rates (> 98%) and that the polymorphic SNPs had good cluster separations. More than 85% of the SNPs were concordant with SNPs obtained from the whole-genome resequencing data. To validate "Yuxin No. 1" for GS, the arrayed genotyping data of 27 individuals from a candidate population and 931 individuals from a reference population were used to calculate the genomic estimated breeding values (GEBVs) for disease resistance to Edwardsiella tarda. There was a 21.2% relative increase in the accuracy of GEBV using the weighted genomic best linear unbiased prediction (wGBLUP), compared to traditional pedigree-based best linear unbiased prediction (ABLUP), suggesting good performance of the "Yuxin No. 1" SNP array for GS. In summary, we developed the "Yuxin No. 1" 50K SNP array, which provides a useful platform for high-quality genotyping that may be beneficial to the genomic selective breeding of Japanese flounder.
Title Author Date Type Operation
Studies of the gonadogenesis, gender differentiation and early stage development of doubled haploid Japanese flounder (Paralichthys olivaceus)
Wang Guixing,Zhu Yimei,Hou Jilun,Zhang Xiaoyan,Wang Yufen,Sun Zhaohui,Jiang Xiufeng,Liu Haijin
Journal Article
Analysis of genetic structure of three third-generation of successive meiogynogenetic families in Japanese flounder, Paralichthys olivaceus
Hou Jilun,Li Chao,Wang Guixing,Zhang Xiaoyan,Sun Zhaohui,Wang Yufen,Jiang Xiufeng,Liu Haijin
Journal Article
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