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Journal Article 22

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denaturing gradient gel electrophoresis (DGGE) 3

terminal restriction fragment length polymorphism (T-RFLP) 3

PCR 2

polymerase chain reaction (PCR) 2

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ATP luminescence detection 1

Angiostrongylus cantonensis 1

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662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 283-285 doi: 10.1007/s11684-008-0053-7

Abstract: diabetes mellitus by D-HPLC mutation screening method and confirmed our results by direct sequencing and PCR-RFLP

Keywords： D-HPLC mutation     development     autoimmune     PCR-RFLP     candidate    

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Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 2,   Pages 194-199 doi: 10.1007/s11783-009-0018-3

Abstract: This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis ofPCR amplified 16S rDNA fragments.The MDA analysis results of T-RFLP profiles coming from I and I both exhibited a significant seasonal

Keywords： terminal restriction fragment length polymorphism (T-RFLP)     bacterial community structure     marine microbial    

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 21-27 doi: 10.1007/s11783-011-0303-9

Abstract: New high-throughput technologies continue to emerge for studying complex microbial communities. In particular, massively parallel pyrosequencing enables very high numbers of sequences, providing a more complete view of community structures and a more accurate inference of the functions than has been possible just a few years ago. In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of specific community members over time, space, or different environmental conditions. In this review, the principles of these two methods and their complementary applications in studying microbial ecology in bioenvironmental systems are discussed. The parallel sequencing of amplicon libraries and using barcodes to differentiate multiple samples in a pyrosequencing run are explained. The best procedures and chemistries for QPCR amplifications are also described and advantages of applying automation to increase accuracy are addressed. Three examples in which pyrosequencing and QPCR were used together to define and quantify members of microbial communities are provided: in the human large intestine, in a methanogenic digester whose sludge was made more bioavailable by a high-voltage pretreatment, and on the biofilm anode of a microbial electrolytic cell. The key findings in these systems and how both methods were used in concert to achieve those findings are highlighted.

Keywords： polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

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Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Strategic Study of CAE 2007, Volume 9, Issue 7,   Pages 76-81

Abstract:

In this paper a new style of polymerase chain reaction(PCR)equipmentIt is the concrete way to reduce the cost of PCR chips and improve the reliability of the chips.The measurement and control scheme for the low-cost PCR chips is designed.The uncertainty of indirect to the temperature of PCR liquid in low-cost PCR chips is also assessed.Its performance in temperature cycling meets the requirements of PCR.

Keywords： microreactors     measurement     control     polymerase chain reaction    

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Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 3,   Pages 488-488 doi: 10.1007/s11783-011-0342-2

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Safety evaluation of microbial pesticide (HaNPV) based on PCR method

Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 2,   Pages 377-384 doi: 10.1007/s11705-018-1777-9

Abstract: Microbial pesticides can prevent and control diseases and pests of crops, and has become one of the important measures to ensure food and environmental safety. However, the potential harm of microbial pesticides to humans and animals is a serious concern at home and abroad. In this paper, we have investigated the infectivity and pathogenicity of a representative of viral microbial pesticides, helicoverpa armigera nuclear polyhedrosis virus (HaNPV), by specific and highly sensitive polymerase chain reaction technology. The results show that HaNPV can be gradually cleared in a short time after getting into blood of experimental rats, and does not infect other tissues or organs of animals; also indicate that the test subjects are not infectious to experimental rats after intravenous injection of HaNPV. Our method has good specificity and repeatability, and could provide an important reference for establishment of infectivity and pathogenicity detection methods for viral microbial pesticides in future.

Keywords： microbial pesticides     HaNPV     acute intravenous injection     infectivity     pathogenicity    

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Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 82-97 doi: 10.1007/s11783-011-0306-6

Abstract: Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

Keywords： denaturing gradient gel electrophoresis (DGGE)     terminal restriction fragment length polymorphism (T-RFLP    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 204-207 doi: 10.1007/s11684-010-0043-4

Abstract: increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCRdiagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR

Keywords： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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Research and application on high-end equipment of biological detection and molecular diagnosis

Peng Niancai,Li Lei,Li Zheng,Miao Baogang,Li Ming,Zhao Yao,Jiang Zhuangde

Strategic Study of CAE 2013, Volume 15, Issue 1,   Pages 57-62

Abstract: social development priorities of the real-time fluorescence quantitative polymerase chain reaction (PCR

Keywords： biological detection     molecular diagnosis     real-time quantitative PCR     ATP luminescence detection    

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Quantitative Analysis of the Effectiveness of Antigen- and Polymerase Chain Reaction-Based Combination Strategies for Containing COVID-19 Transmission in a Simulated Community Article

Qiangru Huang, Yanxia Sun, Mengmeng Jia, Ting Zhang, Fangyuan Chen, Mingyue Jiang, Qing Wang, Luzhao Feng, Weizhong Yang

Engineering 2023, Volume 28, Issue 9,   Pages 234-242 doi: 10.1016/j.eng.2023.01.004

Abstract: Antigen testing and its combination strategies were more efficient than polymerase chain reaction (PCRstrategies is the short turnaround time, which is also a critical factor to be optimized to improve PCR

Keywords： COVID-19     Antigen testing     PCR testing     Testing strategies    

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Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was measured in half smooth tongue sole Cynoglossus semilaevis Günther

Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 50-60

Abstract: Real-time PCR analysis showed that LH mRNA was expressed in all twelve tissues including brain，pituitaryQuantitative real- time PCR was used for analyzing the expression of female C.

Keywords： Cynoglossus semilaevis Günther     LH     cDNA cloning     Quantitative real-time PCR     RIA    

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Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 92-98 doi: 10.1007/s11783-010-0254-6

Abstract: The community dynamics were investigated using specific PCR followed by terminal restriction fragmentlength polymorphism (T-RFLP) analysis of the gene.The T-RFLP results indicated that during the period of nitrification stability, the AOB community structure

Keywords： ammonia-oxidizing bacteria (AOB)     community dynamics     terminal restriction fragment length polymorphism (T-RFLP    

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Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5,   Pages 693-702 doi: 10.1007/s11783-014-0635-3

Abstract: Quantitative PCR (q-PCR) and clone library targeting on gene were used to quantify the abundances of

Keywords： ammonia-oxidizing archaea     ammonia-oxidizing bacteria     quantitative PCR     clone library     plateau    

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Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords： quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

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Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1136-1

Abstract:

• Quantitative global ARGs profile in dialysis water was investigated.

• Totally 35 ARGs were found in the dialysis treatment train.

• 29 ARGs (highest) were found in carbon filtration effluent.

• erm and mtrD-02 occurred in the final effluent.

• The effluent was associated with health risks even after RO treatment.

Keywords： Dialysis water     Treatment process     Antibiotic resistance gene     High-throughput quantitative PCR     Horizontal    

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Title Author Date Type Operation

662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Journal Article

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Journal Article

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Journal Article

Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Journal Article

Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Journal Article

Safety evaluation of microbial pesticide (HaNPV) based on PCR method

Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian

Journal Article

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Journal Article

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Journal Article

Research and application on high-end equipment of biological detection and molecular diagnosis

Peng Niancai,Li Lei,Li Zheng,Miao Baogang,Li Ming,Zhao Yao,Jiang Zhuangde

Journal Article

Quantitative Analysis of the Effectiveness of Antigen- and Polymerase Chain Reaction-Based Combination Strategies for Containing COVID-19 Transmission in a Simulated Community

Qiangru Huang, Yanxia Sun, Mengmeng Jia, Ting Zhang, Fangyuan Chen, Mingyue Jiang, Qing Wang, Luzhao Feng, Weizhong Yang

Journal Article

Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was measured in half smooth tongue sole Cynoglossus semilaevis Günther

Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao

Journal Article

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Journal Article

Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article

Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

Journal Article