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期刊论文 5

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2021 1

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免疫学 1

化疗敏感性 1

吉西他滨 1

复发 1

外周血单核细胞 1

肝内胆管癌 1

肝癌 1

肝移植 1

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Strategies of minimally invasive treatment for intrahepatic and extrahepatic bile duct stones

null

《医学前沿（英文）》 2017年 第11卷 第4期   页码 576-589 doi: 10.1007/s11684-017-0536-5

摘要：

Cholelithiasis is a kind of common and multiple diseases. In recent years, traditional laparotomy has been challenged by a minimally invasive surgery. Through literature review, the therapeutic method, effect, and complications of minimally invasive treatment of intrahepatic and extrahepatic bile duct stones by combining our practical experience were summarized as follows. (1) For intrahepatic bile duct stones, the operation may be selected by laparoscopic liver resection, laparoscopic common bile duct exploration (LCBDE), or percutaneous transhepatic cholangioscopy. (2) For concomitant gallstones and common bile duct stones, the surgical approach can be selected as follows: laparoscopic cholecystectomy (LC) combined with endoscopic sphincterotomy (EST) or endoscopic papillary balloon dilatation, LC plus laparoscopic transcystic common bile duct exploration, LC plus LCBDE, and T-tube drainage or primary suture. (3) For concomitant intrahepatic and extrahepatic bile duct stones, laparoscopic liver resection, choledochoscopy through the hepatic duct orifice on the hepatectomy cross section, LCBDE, EST, and percutaneous transhepatic cholangioscopic lithotripsy could be used. According to the abovementioned principle, the minimally invasive treatment approach combined with the surgical technique and equipment condition will be significant in improving the therapeutic effect and avoiding the postoperative complications or hidden dangers of intrahepatic and extrahepatic bile duct stones.

关键词： laparoscopy     choledochoscopy     duodenoscopy     cholelithiasis     minimally invasive treatment    

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Biological features of intrahepatic CD4+CD25+ T cells in the naturally tolerance of rat liver transplantation

LU Ling, ZHANG Feng, PU Liyong, YAO Aihua, YU Yue, SUN Beicheng, LI Guoqiang, WANG Xuehao

《医学前沿（英文）》 2007年 第1卷 第4期   页码 373-376 doi: 10.1007/s11684-007-0072-9

摘要： The biological features of intrahepatic CD4CD25 T regulatory cells in the naturally tolerance of rat liver transplantation were explored. Orthotopic liver transplantation was performed in two allogeneic rat strain combinations, one with fatal immunosuppression despite a complete major histo compatibility complex mismatch. The subjects were divided into three groups according to different donors and recipients [Tolerance group: LEW-to-DA; Rejection group: DA-to-LEW; Syngegnic group (control group): DA-to-DA]. The proportion of intrahepatic CD4CD25 T cells from three groups was determined by flow cytometry (FCM) in different time. The intrahepaitc CD4CD25 T cells were isolated by magnetic activated cell sorting (MACS) method and iden tified by FCM. The Foxp3 mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). And their suppression on the proliferation of CD4CD25 T effector cells was analyzed by cell proliferation assay . Beginning immediately after transplantation, the proportion of Treg cells increased over time in both allogeneic groups but was significantly greater in the Rejection group. The proportion of Treg cells declined after day 5, and such reduction was more dramatic in the Rejection group than in the Tolerance group. Animals in the Tolerance group showed a second increase in the proportion after day 14. Intrahepatic CD4CD25 T cells isolated from spontaneous tolerance models inhibited the proliferation of mixed lymphocyte reaction. The purity of CD4CD25 T cells sorted by MACS was 86%–93%. The CD4CD25 T cells could specifically express the Foxp3 gene compared with CD4CD25 T cells. , the spleen cells from LEW rats can irritate the proliferation of CD4CD25 T cells more obviously than the syngegnic spleen cells. CD4CD25 Tr cells could suppress the proliferation of CD4CD25 T cells, but the inhibition was reversed by exogenous IL-2 (200 U/mL). The CD4CD25 T regulatory cells specifically express the Foxp3 gene, which may play an important role in the induction of liver transplantation tolerance by suppressing the reaction of effective T cells.

关键词： magnetic     LEW-to-DA     effector     MACS     cytometry    

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To restrict indication for stenting of the inferior vena cava and liver transplantation in patients with Budd-Chiari syndrome

WANG Zhonggao

《医学前沿（英文）》 2007年 第1卷 第2期   页码 130-135 doi: 10.1007/s11684-007-0025-3

摘要： The Budd-Chiari syndrome (BCS) used to be a very rare disorder and lacked a means of relieving. However, various shuntings and radical procedures have emerged in the last three decades with quite encouraging outcomes. Recent minimally invasive therapies, such as stenting of the inferior vena cava (IVC), are simple and easy to handle. However, it has been realized that the IVC stent may compromise the hepatic veins and cause catastrophic consequences, thus the indication for IVC stenting should be reevaluated and especially not to be overused. Instead, it should be applied by percutaneous transangiography (PTA) only, which may be repeated when necessary. The BCS in China predominantly belongs to the IVC type rather than the intrahepatic type in Western; the outcome from its conventional therapy, at least for the moment, is better than that of liver transplantation. It is thus suggested that, before liver transplantation is decided, the conventional means for managing BCS be considered. The final suggestion is to restrict the indication both for stenting of the IVC and liver transplantation. In this paper, ten examples are given with figures.

关键词： Budd-Chiari syndrome     conventional     intrahepatic     percutaneous transangiography     necessary    

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肝内区域免疫对肝移植后肿瘤复发的影响 Review

刘江, 卢宠茂, 万钧

《工程（英文）》 2022年 第10卷 第3期   页码 57-64 doi: 10.1016/j.eng.2021.11.012

摘要：

肝脏恶性肿瘤是肝移植的一个主要适应症，但是肝移植后肿瘤复发却是影响受体长期生存的一个严峻临床挑战。肿瘤生物学特征、分期和移植后的免疫抑制状态一直被认为是肝癌复发的危险因素。而越来越多的证据表明，肝脏缺血再灌注（IR）对同种异体移植物的损伤则为肝移植后的肿瘤细胞侵袭性、转移性
提供了有利的免疫微环境。在活体肝移植中，边缘移植物（如小体积或脂肪移植物）的严重损伤与较低的无复发生存率之间的相关性，证实了IR 损伤与肿瘤复发之间的关联。IR 可引起肝内免疫微环境重构，包括恶化移植物损伤的促炎反应和加快组织修复的抗炎反应。然而，肝内区域免疫对移植后肿瘤复发的作
用尚不清晰。本文详述了IR 损伤诱导的肝内体液微环境和调节性区域免疫微环境，以及它们如何影响肝移植后肿瘤复发的最新研究进展。综合理解移植后肝内区域免疫，将为移植后肿瘤复发提供精准诊断、治疗和预后预测的新策略。

关键词： 肝移植     肝癌     复发     免疫学    

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外周血免疫特征预测肝内胆管癌患者化疗敏感性 Article

吴彤, 杨应成, 郑博, 施学兵, 李炜, 马文聪, 王珊, 李祉萱, 朱妍静, 吴建民, 汪凯婷, 赵燕, 伍睿, 隋成军, 莘似韵, 吴璇, 陈磊, 袁振刚, 王红阳

《工程（英文）》 2021年 第7卷 第10期   页码 1381-1393 doi: 10.1016/j.eng.2021.01.014

摘要：

肝内胆管癌（ICC）是第二常见肝癌。化疗仍然是晚期ICC患者的主要治疗方法，但是化疗敏感性因人而异。在本研究中，我们使用飞行时间质谱仪（CyTOF）在单细胞水平上建立ICC患者在化疗前、化疗中和化疗后指定时间点外周血单核细胞（PBMC）的免疫谱；应用多重免疫荧光染色展现某些特定免疫亚群的空间分布；使用组织微阵列（TMA）评估患者预后。本研究共招募了20名接受吉西他滨（GEM）治疗的ICC患者，其中8例对化疗反应良好（R），12例无反应（NR）。我们观察到化疗后患者PBMC组成发生了巨大变化，比如CD4/CD8 双阳性T细胞（DPT）水平增加。治疗前CD4+CD45RO+CXCR3+T细胞水平较高的患者对化疗反应良好。我们的研究确定了T细胞亚群的百分比与患者化疗反应性之间存在正相关关系，这表明通过评估PBMC中细胞亚群的比例来预测ICC患者化疗反应性是可行的。

关键词： 肝内胆管癌     吉西他滨     化疗敏感性     外周血单核细胞    

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标题 作者 时间 类型 操作

Strategies of minimally invasive treatment for intrahepatic and extrahepatic bile duct stones

null

期刊论文

Biological features of intrahepatic CD4+CD25+ T cells in the naturally tolerance of rat liver transplantation

LU Ling, ZHANG Feng, PU Liyong, YAO Aihua, YU Yue, SUN Beicheng, LI Guoqiang, WANG Xuehao

期刊论文

To restrict indication for stenting of the inferior vena cava and liver transplantation in patients with Budd-Chiari syndrome

WANG Zhonggao

期刊论文

肝内区域免疫对肝移植后肿瘤复发的影响

刘江, 卢宠茂, 万钧

期刊论文

外周血免疫特征预测肝内胆管癌患者化疗敏感性

吴彤, 杨应成, 郑博, 施学兵, 李炜, 马文聪, 王珊, 李祉萱, 朱妍静, 吴建民, 汪凯婷, 赵燕, 伍睿, 隋成军, 莘似韵, 吴璇, 陈磊, 袁振刚, 王红阳

期刊论文