Engineering >> 2024, Volume 33, Issue 2 doi: 10.1016/j.eng.2023.09.001
Ionically Imprinting-Based Copper (Ⅱ) Label-Free Detection for Preventing Hearing Loss
a The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China
b State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China
c Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China
d Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
e Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
f Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China
g Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100101, China
h Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China
# These authors contributed equally to this work.
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Abstract
Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmol∙L–1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.
SupplementaryMaterials