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Engineering >> 2024, Volume 33, Issue 2 doi: 10.1016/j.eng.2023.09.001

Ionically Imprinting-Based Copper (Ⅱ) Label-Free Detection for Preventing Hearing Loss

a The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China
b State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China
c Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China
d Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
e Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
f Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China
g Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100101, China
h Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China

# These authors contributed equally to this work.

Received: 2023-07-15 Revised: 2023-08-21 Accepted: 2023-09-03 Available online: 2023-09-15

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Abstract

Copper is a microelement with important physiological functions in the body. However, the excess copper ion (Cu2+) may cause severe health problems, such as hair cell apoptosis and the resultant hearing loss. Therefore, the assay of Cu2+ is important. We integrate ionic imprinting technology (IIT) and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads (IIHBs) as a low-cost and high-specificity platform for Cu2+ detection. The IIHBs have a macroporous microstructure, uniform size, vivid structural color, and magnetic responsiveness. When incubated in solution, IIHBs recognize Cu2+ and exhibit a reflective peak change, thereby achieving label-free detection. In addition, benefiting from the IIT, the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100 μmolL–1. These features indicated that the developed IIHBs are promising candidates for Cu2+ detection, particularly for the prevention of hearing loss.

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