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Prokaryotic expression and purification of fusion protein HSP70-EGFP and its application in the study

QU Ping, SUI Yanfang, MA Jiahai, CHEN Guangsheng, LIU Libing, CHEN Jiankang, LIU Fang'e

Frontiers of Medicine 2008, Volume 2, Issue 1, Pages 82-86 doi: 10.1007/s11684-008-0015-0

Abstract: Fusion protein HSP70-EGFP was prokaryotically expressed, isolated and purified.Group 1 and 2 were respectively incubated for 30 min. with HSP70-EGFP and EGFP.Flow cytometry (FCM) was adopted to detect the amount of DCs with EGFP inside.There were 5 types in the experiment: LPS, inactive LPS, HSP70-EGFP, EGFP and no antigen.HSP70-EGFP may promote DCs to secret cell factor IL-12.

Keywords： significant difference irradiation peripheral preliminary EGFP

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Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase

Shuaishuai WU,Heng CHEN,Yingzheng WANG,Hui GAO,Shenming ZENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1, Pages 84-89 doi: 10.15302/J-FASE-2015046

Abstract: the surrounding media, and we found that the number of embryos expressing green fluorescent protein (EGFPCa concentrations in the media were found to affect the efficiency which is ICSI embryos expressing EGFPThese findings reveal a mechanism and pathway involving EGFP expression in ICSI embryos.

Keywords： ICSI calcium DNase I GFP porcine