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Journal Article 1

Year

2008 1

Keywords

4-fluorophenylpyruvate 1

Asp-AT 1

FPPA 1

L-4-Fluorophenylalanine 1

aspartate aminotransferase 1

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Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine

CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 220-223 doi: 10.1007/s11705-008-0041-0

Abstract: L-4-Fluorophenylalanine (FPhe) was prepared from 4-fluorophenylpyruvate (FPPA) catalyzed by aspartateAfter 12 h enzymatic reaction, the FPPA conversion was over 90% and the yield of FPhe could be aboveunder the following optimal conditions: 37°C, pH value range of 5.0–8.0, 5.5 mass ratio of cell to FPPA, 0.6% (w/v) of Tween 80, 7.08 g/L FPPA, and 1.6 of molar ratio of L-Asp to FPPA.

Keywords： L-4-Fluorophenylalanine     Asp-AT     aspartate aminotransferase     4-fluorophenylpyruvate     FPPA    

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Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine

CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping

Journal Article