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Investigation of hydrolysis conditions and properties on protein hydrolysates from flatfish skin
Hua ZHANG, Hongji ZHU, Shipeng WANG, Weihua WANG
Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 3, Pages 303-311 doi: 10.1007/s11705-013-1341-6
Keywords: flatfish skin protein hydrolysates metal chelating activity reducing power
Gan Lingling,Wang Weifang,Gao Chunren,Lei Jilin
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 21-25
In this study, formal dehyde was used to inactivate Edwardsiella tarda, Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri, Aeromonas hydrophila, in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antiserum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune, and the level of antibody is research highest in the tenth week which is inoculated inactivated vaccine of Edwardsiella tarda, and the highest titer of other vaccines are 1:102400, 1:6400, 1:6400, 1:12800, 1:12800, 1:6400, 1:6400, 1:12800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum, and it is very weak when the bacteria react with other antiserum.
Lei Jilin,Yang Zhengyong,Ni Qi,Zhang Hesen
Strategic Study of CAE 2010, Volume 12, Issue 8, Pages 70-78
Keywords: flatfish aquaculture circular economy sustainable development strategies
Title Author Date Type Operation
Investigation of hydrolysis conditions and properties on protein hydrolysates from flatfish skin
Hua ZHANG, Hongji ZHU, Shipeng WANG, Weihua WANG
Journal Article
preparation and application of inactivated vaccine and its antiserum from eight common pathogens of Flatfish
Gan Lingling,Wang Weifang,Gao Chunren,Lei Jilin
Journal Article