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Journal Article 6

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Pseudomonas 2

Pseudomonas stutzeri 1

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¹⁵N tracing technique 1

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Cyclic lipopeptide 1

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Denitrification assimilatively and disimilatively 1

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Selective pseudosolubilization capability of

Fei HUA, Hongqi WANG

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4, Pages 539-551 doi: 10.1007/s11783-013-0498-z

Abstract: Pseudosolubilized ability of sp. DG17 on -alkanes, role of biosurfactants in -octadecane uptake and trans-membrane transport mechanism of -octadecane were studied by analyzing amount of pseudosolubilized oil components in water phase, and the fraction of radiolabeled C -octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C to C of -alkanes. In -octadecane broth, pseudosolubilized -octadecane could be accumulated as long as pseudosolubilized rate was faster than mineralization rate of substrate, and the maximum concentration of pseudosolubilized -octadecane achieved to 45.37 mg·L . All of these results showed that sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of -octadecane.

Keywords： Pseudomonas alkane uptake pseudosolubilization trans-membrane transport

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Rhamnolipid synthesis and production with diverse resources

Qingxin Li

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 27-36 doi: 10.1007/s11705-016-1607-x

Abstract: Rhamnolipids are one of the most effective biosurfactants that are of great interest in industrial applications such as enhancing oil recovery, health care, cosmetics, pharmaceutical processes, food processing, detergents for protein folding, and bioremediation due to their unique characteristics such as low toxicity, surface active property to reduce surface/interfacial tensions, and excellent biodegradability. The genes and metabolic pathways for rhamnolipid synthesis have been well elucidated, but its cost-effective production is still challenging. , the most powerful rhamnolipid producer, is an opportunistic pathogen, which limits its large scale production and applications. Rhamnolipid production using engineered strains other than such as and has received much attention. The highest yield of rhamnolipids is achieved when oil-type carbon sources are used, but using cheaper and renewable carbon sources such as lignocellulose would be an attractive strategy to reduce the production cost of rhamnolipids for various industrial applications.

Keywords： biosurfactant rhamnolipid Pseudomonas waste surface tension

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Production of rhamnolipids-producing enzymes of

Kata Kiss,Wei Ting Ng,Qingxin Li

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 133-138 doi: 10.1007/s11705-017-1637-z

Abstract: Rhamnolipids are a class of biosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in . Four of the 5 proteins except RhlC were successfully over-expressed in and three of them (RhlA, RhlB and RhlI) were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins to favor the construction of super strains for rhamnolipids production.

Keywords： rhamnolipids Pseudomonas RhlA RhlB RhlI protein folding

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Toxic effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure and gene expression

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 5, doi: 10.1007/s11783-021-1391-9

Abstract:

• OBS inhibited the growth of P. stutzeri and destroyed its structure.

Keywords： Sodium perfluorononyloxy-benzenesulfonate Aerobic denitrification Pseudomonas stutzeri Ecotoxicity

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Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity Article

Yi-Lin Gu, Jun-Zhou Li, Yan Li, Shen Cong, Jing Wang, Yi-Nan Ma, Hai-Lei Wei

Engineering 2023, Volume 28, Issue 9, Pages 153-165 doi: 10.1016/j.eng.2023.05.016

Abstract: Pseudomonas-CLPs (Ps-CLPs) are extremely diverse in structure and biological activity.Here, we identify medpeptin, a novel CLP from Pseudomonas mediterranea that consists of 22 amino

Keywords： Pseudomonas Cyclic lipopeptide Cell death Leucine-rich repeat extension (LRX) Medpeptin Receptor-like

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LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 4, doi: 10.1007/s11783-020-1349-3

Abstract: Abstract • Pseudomonas mendocina was first reported for aerobic nitrate removal. • It removed 90% oftreatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas

Keywords： Pseudomonas mendocina Aerobic nitrate removal ¹⁵N tracing technique Denitrification assimilatively