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transgenic mice 4

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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 87-96 doi: 10.15302/J-FASE-2016087

Abstract: that the pMAR-CAG-FST-IRES-AcGFP1-polyA-MAR (pMAR-FST) vector had higher capacity to form monoclonal transgenicRemarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higherExogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F .mice was significantly greater than in wild-type mice, with larger muscles in fore and hind limbs of transgenicmice. pMAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner

Keywords： safety of transgenic     bicistron gene transfer body     transgenic mice     muscle development    

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Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Strategic Study of CAE 2002, Volume 4, Issue 8,   Pages 34-39

Abstract: It described advances of transgenic breeding of rapeseed (Brassica napus) in Oil Crops Institute, HunanThe. paper dealt with Bt-transgenic insect-resistant rapeseed line, transgenic sterile line, restorerline and their hybrid, transgenic high oleic acid content rapeseed line and transgenic high oil content

Keywords： rapeseed     transgenic     breeding    

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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Strategic Study of CAE 2016, Volume 18, Issue 3,   Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018

Abstract: the US Food and Drug Administration (FDA) approved the "all fish" growth hormone (gh) transgenicgenetically engineered livestock approved for human consumption and is considered as breakthrough of transgenicThe US FDA made this landmark decision after 20 years rigorous bio-safety assessment of the transgenicBoth the scientific research behind the creation of this transgenic product and the active participation

Keywords： transgenic     Atlantic salmon     industrialization     enlightenments    

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenicThese results have paved the way for future production of marker-free transgenic resistant to PEDV and

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationSubsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenicanalysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenicOur results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC)     transgenic    

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conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 158-173 doi: 10.15302/J-FASE-2014015

Abstract: Bovine IgM was highly expressed in selected transgenic lines, and it largely inhibited rearrangementsAlthough the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantlyThese transgenic mice also exhibited a lower frequency of unique complementarity determining region 3Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressingFinally, we showed that the transgenic mice were deficient in a specific antibody response to antigen

Keywords： bovine Ig μ heavy-chain     transgenic mice     B cell development     allelic exclusion     immune response     Ig repertoire    

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Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy

Maliwan NACONSIE,Peng ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4,   Pages 285-294 doi: 10.15302/J-FASE-2016119

Abstract: As a major staple food source in Africa and other tropical developing countries, cassava ( ) provides basic sustenance for many subsistence farmers. However, cassava roots mainly accumulate starch with limited contribution of other nutrients such as proteins and vitamins. Also, two viral diseases, cassava mosaic disease (CMD) and cassava brown streak disease (CBSD), cause great losses in cassava production in sub-Saharan Africa and the Indian sub-continent. Genetic engineering provides promising approaches to improve nutritional value and increase resistance to viral diseases in cassava. This report presents several successful case studies on engineering protein content by overexpression of nutritious storage proteins and improving cassava resistance to viral diseases by RNA interference. Perspectives on the sustainable acquisition of new knowledge and development of biotechnology to solve these bottlenecks are discussed.

Keywords： Manihot esculenta     protein enhancement     RNA interference     transgenesis     virus resistance    

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Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 314-320 doi: 10.15302/J-FASE-2014038

Abstract: This study was conducted to systematically assess the reproductive performance of transgenic ewes.In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parametersand biochemical parameters determined from the blood samples did not differ significantly between the transgenicMoreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes.Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA

Keywords： TLR4 transgenic ewe     safety assessment     reproductive trait     oocyte     DNA methylation    

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Functional characterization of caffeic acid

Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 1,   Pages 98-107 doi: 10.15302/J-FASE-2017198

Abstract: Transgenic switchgrass plants generated by an RNAi approach were further employed to study the functioncoupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenicbiochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic

Keywords： biofuel crop     caffeic acid O-methyltransferase     forage     lignin     Panicum virgatum     switchgrass     transgenic    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicgenomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Lycium barbarum L.-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation

Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,

Engineering doi: 10.1016/j.eng.2023.09.007

Abstract: In addition, transgenic Nicotiana benthamiana (N. benthamiana) leaves overexpressing miR162a were developedagrobacterium infiltration method. microCT and tartrate-resistant acid phosphatase staining confirmed that transgenicLycium barbarum L. reinforces kidney essence, thereby strengthening the bone. miR162a expressed by transgenic

Keywords： Traditional Chinese medicine     Lycium barbarum     L     miR162a     Osteoporosis     Nuclear receptor corepressor     Transgenic    

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Mechanisms of Steatosis-Derived Hepatocarcinogenesis: Lessons from HCV Core Gene Transgenic Mice Review

Pan Diao, Fangping Jia, Xiaojing Wang, Xiao Hu, Takefumi Kimura, Takero Nakajima, Toshifumi Aoyama, Kyoji Moriya, Kazuhiko Koike, Naoki Tanaka

Engineering 2021, Volume 7, Issue 12,   Pages 1797-1805 doi: 10.1016/j.eng.2021.08.019

Abstract: Transgenic mice carrying the HCV core gene exhibited age-dependent insulin resistance, hepatic steatosis), or cholesterol, were found to influence hepatic steatogenesis and tumorigenesis in HCV core gene transgenicIn this review, we summarize the findings obtained from mouse models transgenic for the HCV genome, witha special focus on HCV core gene transgenic mice, and discuss the mechanisms of steatogenesis and hepatocarcinogenesis

Keywords： Steatosis     Hepatocellular carcinoma     Trans fatty acid     Saturated fatty acid     Dietary restriction     HCV core protein    

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Title Author Date Type Operation

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Journal Article

Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Journal Article

Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Journal Article

Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy

Maliwan NACONSIE,Peng ZHANG

Journal Article

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Journal Article

Functional characterization of caffeic acid

Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Lycium barbarum L.-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation

Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,

Journal Article

Mechanisms of Steatosis-Derived Hepatocarcinogenesis: Lessons from HCV Core Gene Transgenic Mice

Pan Diao, Fangping Jia, Xiaojing Wang, Xiao Hu, Takefumi Kimura, Takero Nakajima, Toshifumi Aoyama, Kyoji Moriya, Kazuhiko Koike, Naoki Tanaka

Journal Article