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Journal Article 9

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PD-L1 2

immunotherapy 2

macrophage 2

2-DG 1

In vivo 1

N-glycan structures 1

Biomimetic intelligent catalysis 1

CD47 1

Inflammation 1

Macrophage 1

Macrophage activation 1

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Macrophage–Fe3O4@PLGA particles 1

Multidrug-resistant Escherichia coli 1

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Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial

Frontiers of Medicine 2023, Volume 17, Issue 4,   Pages 781-795 doi: 10.1007/s11684-023-0986-x

Abstract: Tear film hyperosmolarity plays a core role in the development of dry eye disease (DED) by mediating the disruption of ocular surface homeostasis and triggering inflammation in ocular surface epithelium. In this study, the mechanisms involving the hyperosmolar microenvironment, glycolysis mediating metabolic reprogramming, and pyroptosis were explored clinically, in vitro, and in vivo. Data from DED clinical samples indicated that the expression of glycolysis and pyroptosis-related genes, including PKM2 and GSDMD, was significantly upregulated and that the secretion of IL-1β significantly increased. In vitro, the indirect coculture of macrophages derived from THP-1 and human corneal epithelial cells (HCECs) was used to discuss the interaction among cells. The hyperosmolar environment was found to greatly induce HCECs’ metabolic reprogramming, which may be the primary cause of the subsequent inflammation in macrophages upon the activation of the related gene and protein expression. 2-Deoxy-d-glucose (2-DG) could inhibit the glycolysis of HCECs and subsequently suppress the pyroptosis of macrophages. In vivo, 2-DG showed potential efficacy in relieving DED activity and could significantly reduce the overexpression of genes and proteins related to glycolysis and pyroptosis. In summary, our findings suggested that hyperosmolar-induced glycolytic reprogramming played an active role in promoting DED inflammation by mediating pyroptosis.

Keywords： dry eye disease     glycolytic reprogramming     pyroptosis     inflammation     2-DG    

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Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Engineering doi: 10.1016/j.eng.2023.05.022

Abstract: selective biocatalytic property of macrophages, consisting of an intelligent controlling center (a living macrophage

Keywords： Multidrug-resistant Escherichia coli     Macrophage–Fe3O4@PLGA particles     Biomimetic intelligent catalysis    

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by Transcriptomics and Multiscale Bioassays of Active Components in Xuanfeibaidu Formula to Suppress Macrophage-Mediated Article

Lu Zhao, Hao Liu, Yingchao Wang, Shufang Wang, Dejin Xun, Yi Wang, Yiyu Cheng, Boli Zhang

Engineering 2023, Volume 20, Issue 1,   Pages 63-76 doi: 10.1016/j.eng.2021.09.007

Abstract: Transcriptomic profiling suggested that genes related to macrophage function were differently expressedConsequently, the effects of XFBD on macrophage activation and mobilization were investigated in a macrophageXFBD exerts strong inhibitory effects on both macrophage activation and migration.em>, and Citri grandis Exocarpium rubrum, were then found to strongly downregulate macrophageArtemisiae annuae Herba and Ephedrae Herba were found to substantially inhibit endogenous macrophage

Keywords： Xuanfeibaidu Formula     Multimodal identification     Inflammation     Macrophage activation     Macrophage migration    

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Applications of atomic force microscopy in immunology

Jiping Li, Yuying Liu, Yidong Yuan, Bo Huang

Frontiers of Medicine 2021, Volume 15, Issue 1,   Pages 43-52 doi: 10.1007/s11684-020-0769-6

Abstract: Cellular mechanics, a major regulating factor of cellular architecture and biological functions, responds to intrinsic stresses and extrinsic forces exerted by other cells and the extracellular matrix in the microenvironment. Cellular mechanics also acts as a fundamental mediator in complicated immune responses, such as cell migration, immune cell activation, and pathogen clearance. The principle of atomic force microscopy (AFM) and its three running modes are introduced for the mechanical characterization of living cells. The peak force tapping mode provides the most delicate and desirable virtues to collect high-resolution images of morphology and force curves. For a concrete description of AFM capabilities, three AFM applications are discussed. These applications include the dynamic progress of a neutrophil-extracellular-trap release by neutrophils, the immunological functions of macrophages, and the membrane pore formation mediated by perforin, streptolysin O, gasdermin D, or membrane attack complex.

Keywords： cellular mechanics     atomic force microscopy     neutrophil extracellular trap     macrophage phagocytosis     pore    

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Activation of phagocytosis by immune checkpoint blockade

Chia-Wei Li, Yun-Ju Lai, Jennifer L. Hsu, Mien-Chie Hung

Frontiers of Medicine 2018, Volume 12, Issue 4,   Pages 473-480 doi: 10.1007/s11684-018-0657-5

Abstract:

Inhibition of macrophage-mediated phagocytosis has emerged as an essential mechanism for tumor immuneOne mechanism inhibiting the innate response is the presence of the macrophage inhibitory molecule, signal

Keywords： CD47     PD-1     PD-L1     immunotherapy     TAM     phagocytosis     macrophage    

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Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

Frontiers of Medicine 2023, Volume 17, Issue 2,   Pages 304-316 doi: 10.1007/s11684-022-0964-8

Abstract: The findings indicated that these structures may be closely related to macrophage polarization.

Keywords： macrophage     glycoproteome     glycopeptides     N-glycan structures     PD-L1    

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The Dual Regulatory Roles of Macrophages in Acute Allogeneic Organ Graft Rejection Review

Liang Tan, Yinan Guo, Chang Feng, Yangxiao Hou, Xubiao Xie, Yong Zhao

Engineering 2022, Volume 10, Issue 3,   Pages 21-29 doi: 10.1016/j.eng.2021.10.015

Abstract: component of innate immune cells, macrophages are the predominate infiltrated cells in allografts, and macrophageHowever, some macrophage subpopulations, such as regulatory macrophages, can protect allografts from

Keywords： Macrophages     Transplantation     Tolerance     Rejection     Regulatory macrophage    

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Immunosuppressive tumor microenvironment contributes to tumor progression in diffuse large B-cell lymphoma upon anti-CD19 chimeric antigen receptor T therapy

Frontiers of Medicine 2023, Volume 17, Issue 4,   Pages 699-713 doi: 10.1007/s11684-022-0972-8

Abstract: Anti-CD19 chimeric antigen receptor (CAR)-T cell therapy has achieved 40%–50% long-term complete response in relapsed or refractory diffuse large B-cell lymphoma (DLBCL) patients. However, the underlying mechanism of alterations in the tumor microenvironments resulting in CAR-T cell therapy failure needs further investigation. A multi-center phase I/II trial of anti-CD19 CD28z CAR-T (FKC876, ChiCTR1800019661) was conducted. Among 22 evaluable DLBCL patients, seven achieved complete remission, 10 experienced partial remissions, while four had stable disease by day 29. Single-cell RNA sequencing results were obtained from core needle biopsy tumor samples collected from long-term complete remission and early-progressed patients, and compared at different stages of treatment. M2-subtype macrophages were significantly involved in both in vivo and in vitro anti-tumor functions of CAR-T cells, leading to CAR-T cell therapy failure and disease progression in DLBCL. Immunosuppressive tumor microenvironments persisted before CAR-T cell therapy, during both cell expansion and disease progression, which could not be altered by infiltrating CAR-T cells. Aberrant metabolism profile of M2-subtype macrophages and those of dysfunctional T cells also contributed to the immunosuppressive tumor microenvironments. Thus, our findings provided a clinical rationale for targeting tumor microenvironments and reprogramming immune cell metabolism as effective therapeutic strategies to prevent lymphoma relapse in future designs of CAR-T cell therapy.

Keywords： receptor T     immunotherapy     diffuse large B cell lymphoma     tumor microenvironment     tumor-associated macrophage    

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SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Muscle Regeneration Article

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Engineering doi: 10.1016/j.eng.2023.05.010

Abstract:

Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.

Keywords： In vivo     Short-wave infrared     Skeletal muscle     Macrophage     Regeneration    

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Title Author Date Type Operation

Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial

Journal Article

Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Journal Article

by Transcriptomics and Multiscale Bioassays of Active Components in Xuanfeibaidu Formula to Suppress Macrophage-Mediated

Lu Zhao, Hao Liu, Yingchao Wang, Shufang Wang, Dejin Xun, Yi Wang, Yiyu Cheng, Boli Zhang

Journal Article

Applications of atomic force microscopy in immunology

Jiping Li, Yuying Liu, Yidong Yuan, Bo Huang

Journal Article

Activation of phagocytosis by immune checkpoint blockade

Chia-Wei Li, Yun-Ju Lai, Jennifer L. Hsu, Mien-Chie Hung

Journal Article

Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

Journal Article

The Dual Regulatory Roles of Macrophages in Acute Allogeneic Organ Graft Rejection

Liang Tan, Yinan Guo, Chang Feng, Yangxiao Hou, Xubiao Xie, Yong Zhao

Journal Article

Immunosuppressive tumor microenvironment contributes to tumor progression in diffuse large B-cell lymphoma upon anti-CD19 chimeric antigen receptor T therapy

Journal Article

SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Muscle Regeneration

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Journal Article