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2021 1

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airway inflammation 1

bronchial hyperresponsiveness 1

dual-function 1

eraser 1

fluorescent probe 1

labeling 1

lipoylation modification 1

p65 1

particulate matter 2.5 1

photo-cross-linker 1

sirtuin 1

sirtuin 2 1

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Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating the SIRT2--p65 pathway

Frontiers of Medicine 2021, Volume 15, Issue 5, Pages 750-766 doi: 10.1007/s11684-021-0839-4

Abstract: Sirtuin 2 (SIRT2) is a key modulator in inflammation.

Keywords： particulate matter 2.5 sirtuin 2 p65 airway inflammation bronchial hyperresponsiveness triptolide

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A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1, Pages 121-127 doi: 10.1007/s11705-021-2051-0

Abstract: Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.

Keywords： dual-function fluorescent probe labeling photo-cross-linker lipoylation modification eraser sirtuin