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Tetracycline 2

tetracycline (TC) 2

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Airborne tetracycline resistance genes 1

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Capture loading 1

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Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1274-5

Abstract: Abstract • Sampling parameters with high efficiency was determined. • Operational process to detect airborne ARGs was optimized. • Providing research basis to control airborne ARGs of a laboratory atmosphere Antibiotic resistance genes (ARGs) have been detected in various atmospheric environments. Airborne ARGs transmission presents the public health threat. However, it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples. In this study, an optimized protocol for collecting and detecting airborne ARGs was presented. Experimental results showed that recovery efficiency tended to increase initially and then declined over time, and a range of 550–780 copies/mm2 of capture loading was recommended to ensure that the recovery efficiency is greater than 75%. As the cell walls were mechanically disrupted and nucleic acids were released, the buffer wash protects ARGs dissolution. Three ratios of buffer volume to membrane area in buffer wash were compared. The highest concentrations of airborne ARGs were detected with 1.4 µL/mm2 buffer wash. Furthermore, the majority of the cells were disrupted by an ultrasonication pretreatment (5 min), allowing the efficiency ARGs detection of airborne samples. While, extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments. Therefore, this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments. An optimized sampling method was proposed that the buffer wash was 1.4 µL/mm2 and the ultrasonication duration was 5 min. The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories. The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

Keywords： Airborne tetracycline resistance genes     Filter sampling     Capture loading     Membrane pretreatment     Indoor laboratory    

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Gold modified microelectrode for direct tetracycline detection

Hongtao WANG, Huimin ZHAO, Xie QUAN

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 3,   Pages 313-319 doi: 10.1007/s11783-011-0323-5

Abstract: The residues of tetracycline antibiotics in water have attracted many concerns due to their harmful impactThis paper reports an electrochemical sensor for the determination of tetracycline (TC) by the microelectrodeElectrochemical determination of tetracycline was investigated using microelectrode by cyclic voltammetry

Keywords： microelectrode     tungsten tip     gold colloids     tetracycline    

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Erratum to: Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1289-y

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Effective degradation of tetracycline by mesoporous Bi

Xiaolong CHU,Guoqiang SHAN,Chun CHANG,Yu FU,Longfei YUE,Lingyan ZHU

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 2,   Pages 211-218 doi: 10.1007/s11783-014-0753-y

Abstract: Bi WO was synthesized with a hydrothermal method at different pHs and used for the degradation of tetracycline

Keywords： Bi2WO6     hydrothermal synthesis     tetracycline (TC)     photocatalysis    

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Sorption of tetracycline to sediments and soils: assessing the roles of pH, the presence of cadmium and

Guixiang ZHANG, Xitao LIU, Ke SUN, Ye ZHAO, Chunye LIN

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 421-429 doi: 10.1007/s11783-010-0265-3

Abstract: Batch sorption experiments were conducted to evaluate the sorption behavior of tetracycline (TC, H L)

Keywords： sorption     tetracycline (TC)     pH     cadmium (Cd)     antibiotic    

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Immobilization of NZVI in polydopamine surface-modified biochar for adsorption and degradation of tetracycline

Xiangyu Wang, Weitao Lian, Xin Sun, Jun Ma, Ping Ning

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 4, doi: 10.1007/s11783-018-1066-3

Abstract: itemcontent>

PDA/NZVI@BC exhibits significantly enhanced activity for tetracycline

The mechanism of tetracycline removal by PDA/

Keywords： Biochar     Polydopamine     NZVI     Modification     Tetracycline    

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In situ formation of bimetallic FeNi nanoparticles on sand through green technology: Application for tetracycline

Ravikumar KVG, Debayan Ghosh, Mrudula Pulimi, Chandrasekaran Natarajan, Amitava Mukherjee

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1195-3

Abstract: . • Removal of tetracycline using GS-FeNi in batch and column study. • Both reductive degradation andextract, and these nanoparticles were also formed in situ over quartz sand (GS-FeNi) for removal of tetracycline

Keywords： GS-FeNi nanoparticles     Tetracycline removal     Re-usability     Residual toxicity     Column studies    

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a novel anion exchange group modified hyper-crosslinked resin for the effective adsorption of both tetracycline

Qing ZHOU, Mengqiao WANG, Aimin LI, Chendong SHUANG, Mancheng ZHANG, Xiaohan LIU, Liuyan WU

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 3,   Pages 412-419 doi: 10.1007/s11783-013-0483-6

Abstract: XAD-4 was selected as an adsorbent for comparison to investigate the adsorption behavior of tetracycline

Keywords： high surface area     adsorption     anion exchange     micropollutant     dissolved organic matters    

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Assessing combined toxic effects of tetracycline and P25 titanium dioxide nanoparticles using

Barsha Roy, Khushboo Kadam, Suresh Palamadai Krishnan, Chandrasekaran Natarajan, Amitava Mukherjee

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 1, doi: 10.1007/s11783-020-1298-x

Abstract: Usage of titanium dioxide nanoparticles (TiO2 NPs) and tetracycline (TC) has increased significantly

Keywords： Binary toxicity     Cell viability     Mitotic index     Chromosomal aberration     Reactive oxygen species     Nanoparticles internalization    

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Green Synthesis of Magnetic Adsorbent Using Groundwater Treatment Sludge for Tetracycline Adsorption Article

Zhan Qu, Yaqiong Wu, Suiyi Zhu, Yang Yu, Mingxin Huo, Leilei Zhang, Jiakuan Yang, Dejun Bian, Yi Wang

Engineering 2019, Volume 5, Issue 5,   Pages 880-887 doi: 10.1016/j.eng.2019.06.001

Abstract: The synthesized MA2 was used to adsorb the cationic pollutant tetracycline (TC).

Keywords： Groundwater treatment sludge     Maghemite     Cationic exchange     Adsorption     Tetracycline    

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Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 158-163 doi: 10.1007/s11684-009-0042-5

Abstract: The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associated virus (AAV) was investigated. Recombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinant AAV genome was replicated and packaged with the helper virus HSV1-rc/ΔUL2. The titer of the recombinant rAAV2-tet-off-bFGF was determined by dot-blot assay. MC3T3-E1 cells were infected with rAAV2-tet-off-bFGF. Regulatory effects of Doxycycline (Dox) on bFGF and osteogenic factors were assayed quantitatively by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The physical particle titer of rAAV2-tet-off-bFGF successfully constructed was 1.8×10 vector genomes/mL, and the virus could infect MC3T3-E1 cells effectively. In MC3T3-E1 cells treated with Dox, the expression levels of exogenous bFGF and osteogenic factors declined to varying degrees. It was concluded that rAAV2-tet-off-bFGF could infect MC3T3 cells efficiently, and this recombinant system could be regulated successfully by Dox .

Keywords： tetracycline regulatory system     adeno-associated virus     basic fibroblast growth factor     gene regulation    

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Decoration of CdMoO micron polyhedron with Pt nanoparticle and their enhanced photocatalytic performance in N fixation and water purification

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 12,   Pages 1949-1961 doi: 10.1007/s11705-023-2360-6

Abstract: a novel Pt/CdMoO4 composite photocatalyst for photocatalytic N2 fixation and tetracyclineIn addition, the composite also exhibited excellent performance in tetracycline degradation, with hole

Keywords： photocatalytic N2 fixation     Pt/CdMoO4     tetracycline degradation     Schottky barrier    

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Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments

Mengli Wang, Ruying Li, Qing Zhao

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1127-2

Abstract:

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

Keywords： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic    

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Title Author Date Type Operation

Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

Journal Article

Gold modified microelectrode for direct tetracycline detection

Hongtao WANG, Huimin ZHAO, Xie QUAN

Journal Article

Erratum to: Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

Journal Article

Effective degradation of tetracycline by mesoporous Bi

Xiaolong CHU,Guoqiang SHAN,Chun CHANG,Yu FU,Longfei YUE,Lingyan ZHU

Journal Article

Sorption of tetracycline to sediments and soils: assessing the roles of pH, the presence of cadmium and

Guixiang ZHANG, Xitao LIU, Ke SUN, Ye ZHAO, Chunye LIN

Journal Article

Immobilization of NZVI in polydopamine surface-modified biochar for adsorption and degradation of tetracycline

Xiangyu Wang, Weitao Lian, Xin Sun, Jun Ma, Ping Ning

Journal Article

In situ formation of bimetallic FeNi nanoparticles on sand through green technology: Application for tetracycline

Ravikumar KVG, Debayan Ghosh, Mrudula Pulimi, Chandrasekaran Natarajan, Amitava Mukherjee

Journal Article

a novel anion exchange group modified hyper-crosslinked resin for the effective adsorption of both tetracycline

Qing ZHOU, Mengqiao WANG, Aimin LI, Chendong SHUANG, Mancheng ZHANG, Xiaohan LIU, Liuyan WU

Journal Article

Assessing combined toxic effects of tetracycline and P25 titanium dioxide nanoparticles using

Barsha Roy, Khushboo Kadam, Suresh Palamadai Krishnan, Chandrasekaran Natarajan, Amitava Mukherjee

Journal Article

Green Synthesis of Magnetic Adsorbent Using Groundwater Treatment Sludge for Tetracycline Adsorption

Zhan Qu, Yaqiong Wu, Suiyi Zhu, Yang Yu, Mingxin Huo, Leilei Zhang, Jiakuan Yang, Dejun Bian, Yi Wang

Journal Article

Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Journal Article

Decoration of CdMoO micron polyhedron with Pt nanoparticle and their enhanced photocatalytic performance in N fixation and water purification

Journal Article

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments

Mengli Wang, Ruying Li, Qing Zhao

Journal Article