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Isolation and characterization of a bacterial strain that efficiently degrades sex steroid hormones

JI Shulan, LIU Zhipeng, REN Haiyan, LIU Zhipei

《环境科学与工程前沿（英文）》 2007年第1卷第3期页码 325-328 doi: 10.1007/s11783-007-0055-8

摘要： A bacterial strain, ZY3, growing on sex steroid hormones as the sole source of carbon and energy was isolated from the sewage treatment plant of a prophylactic steroids factory. ZY3 degrades the 3-methoxy-17-hydroxy-1,3,5(10),8(9)--4-estren (MHE). This strain was preliminarily identified as sp. ZY3 according to its morphology and its 16S rRNA gene sequence. During the experimental period (72 h), the optimum temperature, pH and 3-MHE concentration for the degradation of hydride by the strain ZY3 were 35vH, 10 and 10 mg/L, respectively. The degradation rate of the sex steroid hormones increased to 87% and 85% after the addition of maltose and peptone, respectively.

关键词： maltose optimum temperature prophylactic 3-methoxy-17-hydroxy-1 3 5 addition

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17–26.5 GHz 42.5 dBm宽带高效率GaN功率放大器设计 Correspondences

黎明1,2,李智群1,3,郑权2,蔺兰峰2,陶洪琪2

《信息与电子工程前沿（英文）》 2022年第23卷第2期页码 346-350 doi: 10.1631/FITEE.2000513

摘要：该功率放大器采用0.15 μm栅长GaN-HEMT工艺，其工作频段可以覆盖整个K频段，即17–26.5 GHz。该功率放大器采用三级放大拓扑结构，在连续波条件下，测试结果表明，在17–26.5 GHz频带范围内饱和输出功率超过42.5 dBm，平均PAE为30%，在19.8 GHz时PAE达到最大，为32.1%，

关键词： K波段；高效率；宽带；氮化镓（GaN）；功率放大器

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利用cyp17a1缺失的伪雄鲤成功构建全雌鲤群体 Article

翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战

《工程（英文）》 2022年第8卷第1期页码 181-189 doi: 10.1016/j.eng.2021.03.026

摘要：本研究针对雌鲤生长快于雄鲤的两性生长异形的特性，以cyp17a1 为靶点进行了CRISPR/Cas9介导的基因编辑操作，利用cyp17a1 敲除后形成的伪雄鲤，成功构建了全雌鲤群体首先，我们发现无论遗传性别型为XX还是XY，其cyp17a1 缺失鲤个体性腺均发育为精巢，并能正常产精。其次，利用确定的雄性特异DNA分子标记，筛选到cyp17a1 ^-/- XX伪雄个体，其具有正常的精巢发育和精子发生。将全雌群体和对照组雌雄混合群体各500 尾进行同塘生长对比养殖，至8 月龄或12 月龄时随机抽样检测，发现cyp17a1杂合突变雌鲤体重比同塘性别混养对照组分别高6.60%或32.66%

关键词：鲤两性异形生长 cyp17a1 性类固醇激素全雌群体

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Long-term effects of various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes

Seo Yeon Baik, Hyunah Kim, So Jung Yang, Tong Min Kim, Seung-Hwan Lee, Jae Hyoung Cho, Hyunyong Lee, Hyeon Woo Yim, Kun-Ho Yoon, Hun-Sung Kim

《医学前沿（英文）》 2019年第13卷第6期页码 713-722 doi: 10.1007/s11684-018-0661-9

摘要： Few long-term follow-up studies have compared the changes in renal function according to the type of statin used in Korea. We compared the long-term effects of statin intensity and type on the changes in the glomerular filtration rate (GFR). We extracted data of patients who took statin for the first time. We analyzed whether or not different statins affect the changes in GFR at 3 months after baseline and 4 years after. We included 3678 patients and analyzed the changes in GFR. The GFR decreased by 3.2%±0.4% on average 4 years after the first statin prescription, indicating statistically significant deterioration (from 83.5±0.4 mL/min/1.73 m to 79.9±0.4 mL/min/1.73 m , <0.001). When comparing the GFR among different statins, significant differences were observed between atorvastatin and fluvastatin (−5.3%±0.7% vs. 1.2%±2.2%, <0.05) and between atorvastatin and simvastatin (−5.3%±0.7% vs. −0.7%±0.8%, <0.05). In pitavastatin (odds ratio [OR]=0.64, 95% confidence interval [CI]=0.46–0.87, <0.005) and simvastatin (OR=0.69, 95% CI=0.53–0.91, <0.008), the GFR rate that decreased by<60 mL/min/1.73 m was significantly lower than that of atorvastatin. Regarding long-term statin intake, GFR changed with the type of statin. This work is the first in Korea to compare each statin in terms of changes in the GFR after the statin prescription.

关键词： statin glomerular filtration rate HMG-CoA reductase inhibitor chronic kidney disease

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Th17 Cells in autoimmune diseases

null

《医学前沿（英文）》 2015年第9卷第1期页码 10-19 doi: 10.1007/s11684-015-0388-9

摘要：

Th17 cells are a new subset of CD4⁺ T cells involved in the clearance of extracellular pathogens and fungi. Accumulating evidence suggests that Th17 cells and their signature cytokines have a pivotal role in the pathogenesis of multiple autoimmune-mediated inflammatory diseases. Here, we summarize recent research progress on Th17 function in the development and pathogenesis of autoimmune diseases. We also propose to identify new small molecule compounds to manipulate Th17 function for potential therapeutic application to treat human autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, Sj?gren’s syndrome, inflammatory bowel disease, and multiple sclerosis.

关键词： IL-17 Th17 cells RORγt autoimmune diseases posttranslational modification inhibitors

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Haploinsufficiency of Lipin3 leads to hypertriglyceridemia and obesity by disrupting the expression andhypertriglyceridemia and obesity by disrupting the expression and nucleocytoplasmic localization of Lipin1

《医学前沿（英文）》 doi: 10.1007/s11684-023-1003-0

摘要： Lipin proteins including Lipin 1–3 act as transcriptional co-activators and phosphatidic acid phosphohydrolase enzymes, which play crucial roles in lipid metabolism. However, little is known about the function of Lipin3 in triglyceride (TG) metabolism. Here, we identified a novel mutation (NM_001301860: p.1835A>T/p.D612V) of Lipin3 in a large family with hypertriglyceridemia (HTG) and obesity through whole-exome sequencing and Sanger sequencing. Functional studies revealed that the novel variant altered the half-life and stability of the Lipin3 protein. Hence, we generated Lipin3 heterozygous knockout (Lipin3-heKO) mice and cultured primary hepatocytes to explore the pathophysiological roles of Lipin3 in TG metabolism. We found that Lipin3-heKO mice exhibited obvious obesity, HTG, and non-alcoholic fatty liver disorder. Mechanistic study demonstrated that the haploinsufficiency of Lipin3 in primary hepatocytes may induce the overexpression and abnormal distribution of Lipin1 in cytosol and nucleoplasm. The increased expression of Lipin1 in cytosol may contribute to TG anabolism, and the decreased Lipin1 in nucleoplasm can reduce PGC1α, further leading to mitochondrial dysfunction and reduced TG catabolism. Our study suggested that Lipin3 was a novel disease-causing gene inducing obesity and HTG. We also established a relationship between Lipin3 and mitochondrial dysfunction.

关键词： Lipin3 Lipin1 hypertriglyceridemia obesity mitochondrial dysfunction

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Relationship between Th17 cells and allograft rejection

Zhikun ZHENG MM, Jinsong LI MD, Ke JIANG MD,

《医学前沿（英文）》 2009年第3卷第4期页码 491-494 doi: 10.1007/s11684-009-0066-x

摘要： Thl7 cells, a special kind of auxiliary type T cells, can secrete IL-17A, IL-17F, IL-21, IL-22, etc., as a newly discovered T-cell subset in recent years. As a different subset from the Thl and Th2 cells, Th17 cells play an important role in the development of a variety of autoimmune diseases. A current study shows that the IL-6 inflammatory response of the organization in combination with the occurrence of TGF-β can induce the differentiation of Thl7 cells. IL-23 can promote the production of IL17, as well as participate in amplification and maintenance of the survival of IL-l7 generating cells. In this process, STAT3 and ROR-γt are key transcription factors for the growing of Thl7 cells. As our knowledge on Th17 family members is rapidly growing and changing, it will be important to specify their involvement in the induction and regulation of allograft rejection in animal models as well as in clinical settings. Herein, we review the key features of Th17 cells and discuss their potential relevance to transplantation immunity.

关键词： TH17 cells regulation allograft rejection

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sources of dissolved organic matter from wastewater treatment plants and their mediation effect on 17α-ethinylestradiol

《环境科学与工程前沿（英文）》 2023年第17卷第6期 doi: 10.1007/s11783-023-1669-1

摘要：

● EE2 photodegradation behavior in the presence of four WWTPs’ DOM was explored.

关键词： Photosensitivity sources 17α-ethinylestradiol Photodegradation Dissolved organic matter Wastewater treatment plants

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年第8卷第2期

摘要：

Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that MdSIZ1 in apple has a negative relationship with drought tolerance. MdSIZ1 RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H₂O₂ and malondialdehyde contents, and higher catalase activity. In addition, MdSIZ1 RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

关键词： apple / drought tolerance / gene expression / MdSIZ1

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基于玻璃通孔的Ka波段宽带滤波封装天线 Research Article

方针1,2,张继华1,2,3,高莉彬1,2,陈宏伟1,2,李文磊1,2,梁天鹏1,2,蔡旭东1,2,蔡星周3,贾惟聪3,郭欢3,李勇3

《信息与电子工程前沿（英文）》 2023年第24卷第6期页码 916-926 doi: 10.1631/FITEE.2200573

摘要： BPF单元有3对λg/4槽(缺陷微带结构)和两对λg/2 U形缝隙(缺陷地结构)。该天线实现了大带宽和高辐射效率，这可能与玻璃基板的叠加和玻璃通孔馈电有关。

关键词：滤波封装天线；玻璃通孔；三维封装器件；激光键合

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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

《医学前沿（英文）》 2007年第1卷第3期页码 269-273 doi: 10.1007/s11684-007-0051-1

摘要： The aim of this article was to investigate the influence and the related mechanism of the gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3b (GSK-3). The glucose uptake decreased with the increase in gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3 and p38MAPK, increased with overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.

关键词： 3T3-L1 influence resistance receptor substrate-1 transport

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用以产生高纯度涡旋波束的宽带圆极化透射阵 Correspondence

邱靓婕1,2,3,4,李秀萍1,2,3,4,齐紫航1,2,3,4,赵文禹1,2,3,4,黄雨菡5

《信息与电子工程前沿（英文）》 2023年第24卷第6期页码 927-934 doi: 10.1631/FITEE.2200539

摘要：亚波长结构以及双谐振特性确保了透射单元在28.4%的1-dB透射带宽内具有稳定的相移能力。基于此，加工测试了一款由喇叭天线馈电的方形口径透射阵。得益于蜂窝状布阵方式，所设计的透射阵可在28.5 GHz到38 GHz的宽带范围中辐射l=–1且模态纯度高于0.93的涡旋波束。测试的峰值增益为22.3 dBic，3-dB轴比带宽为27.6%。

关键词：轨道角动量；涡旋波束；透射阵；宽带；高纯度

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Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance

《医学前沿（英文）》 2022年第16卷第6期页码 883-895 doi: 10.1007/s11684-022-0919-0

摘要： Metastasis and drug resistance are the leading causes of poor prognosis in patients with osteosarcoma. Identifying the relevant factors that drive metastasis and drug resistance is the key to improving the therapeutic outcome of osteosarcoma. Here, we reported that autophagy was highly activated in metastatic osteosarcoma. We found increased autophagolysosomes in metastatic osteosarcoma cell lines by using electron microscopy, Western blot, and immunofluorescence experiments. We further examined the expression of the autophagy-related genes Beclin1 and LC3B in 82 patients through immunohistochemistry and found that Beclin1 and LC3B were highly related to unfavorable prognosis of osteosarcoma. Knockdown of Beclin1 and LC3B reduced invasion, metastasis, and proliferation in metastatic osteosarcoma cells. In vitro and in vivo studies also demonstrated that inhibiting by 3-MA inhibited cell growth and metastasis. Moreover, we demonstrated that autophagy-related genes were activated by SEs and that the inhibition of SEs by JQ-1 decreased the metastasis of osteosarcoma. Overall, our findings highlighted the association of autophagy with osteosarcoma progression and shed new light on autophagy-targeting therapy for osteosarcoma.

关键词： osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B

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Traces and echoes of De Architectura by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

《机械工程前沿（英文）》 2016年第11卷第1期页码 3-11 doi: 10.1007/s11465-015-0359-5

摘要：

This study aims to investigate the role played by Xu Guangqi (1562–1633), minister of the Ming Dynasty, in the development of European scientific and technical knowledge in China between the 16th and 17th centuries by analyzing a book of Western technology that he wrote, namely, Taixi Shuifa (On Western Hydraulics). Several Western books related to machine knowledge are searched to trace the source of the illustrations in Taixi Shuifa. We found that Archimedes’ screw and Ctesibius’ machine, which are included in Vitruvius’ De Architectura volumes, also appear in the work of Xu Guangqi.

关键词： Vitruvius’ De Architectura Xu Guangqi’s Taixi Shuifa Jesuit missionaries Italian Renaissance drawing representation

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Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K/AKT/DEC1 pathway

LI Yanhua, BI Zhigang

《医学前沿（英文）》 2007年第1卷第1期页码 79-86 doi: 10.1007/s11684-007-0016-4

摘要： The aim of this research was to explore the effects and signaling pathway of ultraviolet-B (UVB) irradiation on the expression of hypoxia-inducible factor 1α (HIF-1α) and transferrin receptor (TfR). HIF-1α protein was measured by Western blot method. Expressions of epidermal growth factor receptor (EGFR), phosphor-EGF-R and TfR after UVB irradiation were determined with flow cytometry. After UVB irradiation, mRNA levels of HIF-1α and TfR were detected by real time-PCR. Results showed that compared with control groups, UVB was able to induce HIF1α and TfR protein expression in a dose- and time-dependent manner in HaCat cells (<0.05). TfR mRNA was expressed in a dose-dependent manner and reached a peak at the 8th hour in HaCat cells (<0.05) whereas HIF-1α mRNA expression was not affected by UVB treatment (>0.05). The EGFR/PI3K/AKT signaling pathway was required for the induction of HIF-1α and TfR expression induced by UVB. UVB induced activation of EGFR in HaCat cells and EGFR regulated expression of TfR and HIF-1α. EGFR (-/-) MEF did not increase the HIF1 expression following UVB irradiation (>0.05). In contrast, EGFR (+/+) MEF strongly enhanced HIF1α expression after UVB irradiation (<0.05). PD153035, a selective inhibitor of EGFR tyrosine kinase, inhibited the TfR protein expression in UVB-treated cells in a dose-dependent manner (P<0.05). PI3K inhibitors, LY294002 and wortmannin, inhibited HIF-1? and TfR expressions induced by UVB (P<0.05). The DEC1 (-/-) Ha-Cat cells did not increase their TfR and HIF-1α expressions following UVB irradiation (>0.05). In contrast, DEC1 (+/+) HaCat cells strongly enhanced TfR and HIF-1? protein expression after UVB irradiation (P<0.05). We conclude that UVB induces TfR and HIF-1αexpressions via EGFR/PI3K/AKT/DEC1 signaling pathway.

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标题作者时间类型操作

Isolation and characterization of a bacterial strain that efficiently degrades sex steroid hormones

JI Shulan, LIU Zhipeng, REN Haiyan, LIU Zhipei

期刊论文

17–26.5 GHz 42.5 dBm宽带高效率GaN功率放大器设计

黎明1,2,李智群1,3,郑权2,蔺兰峰2,陶洪琪2

期刊论文

利用cyp17a1缺失的伪雄鲤成功构建全雌鲤群体

翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战

期刊论文

Long-term effects of various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes