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Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

《环境科学与工程前沿（英文）》 2007年第1卷第3期页码 329-333 doi: 10.1007/s11783-007-0056-7

摘要： Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria, which cause lots of accidents and threatens human health. In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established and used to detect microcystin-LR (MC-LR) in drinking and surface waters. The concentration of coating antigen was 5 ?g/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouse IgG-peroxidase) was 1:3 000, the standard concentration of MC-LR ranged from 0.001 μg/L to 30 μg/L, and o-phenylenediamine was used as substrate. The assay showed high relativity with high performance liquid chromatography (HPLC) with a correlation coefficient of more than 99%. The relative standard deviation was less than 10%, the detection limit was achieved down to 0.01 μg/L and up to 5.1 μg/L. The quantitative detection range was from 0.03 μg/L to 3 μg/L, and the antibody had high specificity for [4-arginine] microcystins. It performed well in spite of the influence of the real samples.

关键词： o-phenylenediamine 4-arginine ic-ELISA substrate chromatography

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世界IC产业的发展趋势及启示

吴广谋,曹杰,王波

《中国工程科学》 2007年第9卷第11期页码 127-131

摘要：

在分析我国集成电路（IC）产业发展过程和现状的基础上，分析了世界IC产业的发展趋势，结合我国 IC产业的特点和世界IC产业的发展趋势，提出了如何加快发展我国IC产业的７点建议，包括：建立松散型纵向结构的IC企业组织模式；加大重点投资、制订优惠政策；优化产业结构，提升产业能力；鼓励整机厂商和 IC设计企业之间的协作；加快发展IC产业集群；建设国家级研发中心

关键词：集成电路(IC) 系统集成芯片(SOC) 产业组织

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Preparation of hapten-specific monoclonal antibody for cadmium and its ELISA application to aqueous samples

Huan HE, Bo TANG, Cheng SUN, Shaogui YANG, Weijuan ZHENG, Zichun HUA

《环境科学与工程前沿（英文）》 2011年第5卷第3期页码 409-416 doi: 10.1007/s11783-011-0349-8

摘要： High-affinity and specific monoclonal antibodies against cadmium-ethylene diamine tetraacetic acid (EDTA) complex have been produced using the hybridoma technique. A hapten was synthesized and characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and UV-Vis. Competitive enzyme-linked immunosorbent assay (ELISA) for quantitative detection of cadmium in aqueous sample was developed. The monoclonal antibody with high level of binding affinity for Cd-IEDTA-BSA and high specificity for soluble Cd-EDTA complex showed less than 0.99% cross-reactivity with other 11 metals. The limit of detection was 0.10 μg·L , and the effective linear range was 10 –10 μg·L . The intra- and inter-assay coefficient variations were 1.5%–6.3% and 3.2%–7.4%, respectively. The spike recovery in different water samples were between 98.5% and 110.3%. The detection limit of this assay was well below the allowable concentration of cadmium (3 μg·L ), and the working range was wider than that of other methods which showed the range of 2.19–86.38 and 0–10 μg·L . The competitive ELISA established in this paper was sensitive and accurate in the screening of cadmium in aqueous samples. The results will lay a solid foundation for construction of an immunoassay kit for cadmium.

关键词： cadmium hapten monoclonal antibody enzyme-linked immunosorbent assay (ELISA)

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自主创新技术学习与产业竞争力的提高——以台湾IC业为例

卢锐,盛昭瀚

《中国工程科学》 2007年第9卷第8期页码 35-39

摘要：

自主创新、技术学习是台湾集成电路（IC）产业遵循比较优势的产业政策和技术政策的结果，是基于本土市场的自主创新以及企业在技术学习上的努力，

关键词：技术学习自主创新产业竞争力 IC产业

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工程监理委托代理关系中激励约束与参与约束的研究

秦旋

《中国工程科学》 2007年第9卷第4期页码 45-49

摘要：

基于委托－代理理论对工程监理委托－代理关系中的激励约束与参与约束进行若干拓展研究，与参与约束（IR）相比，激励相容约束（IC）更为重要，

关键词：工程监理业主工程师委托代理激励相容约束参与约束

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measurement of the thickness of top Cu film of the multilayer interconnects in the integrated circuit (IC

Zilian QU,Yonggang MENG,Qian ZHAO

《机械工程前沿（英文）》 2015年第10卷第1期页码 1-6 doi: 10.1007/s11465-015-0325-2

摘要：

This paper proposes a new eddy current method, named equivalent unit method (EUM), for the thickness measurement of the top copper film of multilayer interconnects in the chemical mechanical polishing (CMP) process, which is an important step in the integrated circuit (IC) manufacturing. The influence of the underneath circuit layers on the eddy current is modeled and treated as an equivalent film thickness. By subtracting this equivalent film component, the accuracy of the thickness measurement of the top copper layer with an eddy current sensor is improved and the absolute error is 3 nm for sampler measurement.

关键词： CMP eddy current multilayer wafer Cu interconnects equivalent unit

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Synthesis of haptens and production of antibodies to bisphenol A

Xiya ZHANG, Xiaoyun DONG, Sijun ZHAO, Yuebin KE, Kai WEN, Suxia ZHANG, Zhanhui WANG, Jianzhong SHEN

《农业科学与工程前沿（英文）》 2017年第4卷第3期页码 366-372 doi: 10.15302/J-FASE-2017132

摘要： Three immunizing haptens of bisphenol A (BPA), including two new haptens, were used to produce highly sensitive and specific polyclonal antibodies. The spacer arms of haptens for coupling to the protein carrier were located at different positions in BPA, and different length spacer arms were tested. Highly sensitive polyclonal antibodies were obtained and characterized using indirect competitive enzyme-linked immunosorbent assay (icELISA). Under optimized conditions, the half maximal inhibitory concentration (IC ) value of the best polyclonal antibody was 2.1 mg·L , based on coating heterogeneous antigens, and this optimal polyclonal antibody was highly sensitive toward BPA and displayed negligible cross-reactivity with bisphenol B and bisphenol E. A sensitive icELISA method utilizing the polyclonal antibody was developed for the determination of BPA in milk. In spiked samples (5, 10 and 20mg·L ), the recovery ranged from 80% to 102% with a coefficient of variation (CV) value below 15.8%. The limit of detection of icELISA was 1.95mg·L . These results indicate that the icELISA method is suitable for the detection of BPA in milk.

关键词： bisphenol A cross-reactivity hapten indirect competitive ELISA polyclonal antibody