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Co-application of energy uncoupling and ultrafiltration in sludge treatment: Evaluations of sludge reduction, supernatant

An Ding, Yingxue Zhao, Zhongsen Yan, Langming Bai, Haiyang Yang, Heng Liang, Guibai Li, Nanqi Ren

《环境科学与工程前沿（英文）》 2020年 第14卷 第4期 doi: 10.1007/s11783-020-1238-9

摘要： Effects of metabolic uncouplers addition on sludge reduction were carried out. TCS addition effectively inhibited ATP synthesis and reduced sludge yield. The effluent quality such as TOC and ammonia deteriorated but not significantly. Suitable dosage retarded biofouling during sludge water recovery by UF membrane. Energy uncoupling is often used for sludge reduction because it is easy to operate and does not require a significant amount of extra equipments (i.e. no additional tank required). However, over time the supernatant extracted using this method can deteriorate, ultimately requiring further treatment. The purpose of this study was to determine the effect of using a low-pressure ultrafiltration membrane process for sludge water recovery after the sludge had undergone an energy uncoupling treatment (using 3,3′,4′,5-tetrachlorosalicylanilide (TCS)). Energy uncoupling was found to break apart sludge floc by reducing extracellular polymeric substances (EPS) and adenosine triphosphate (ATP) content. Analysis of supernatant indicated that when energy uncoupling and membrane filtration were co-applied and the TCS dosage was below 30 mg/L, there was no significant deterioration in organic component removal. However, ammonia and phosphate concentrations were found to increase as the concentration of TCS added increased. Additionally, due to low sludge concentrations and EPS contents, addition of 30–60 mg/L TCS during sludge reduction increased the permeate flux (two times higher than the control) and decreased the hydraulic reversible and cake layer resistances. In contrast, high dosage of TCS aggravated membrane fouling by forming compact fouling layers. In general, this study found that the co-application of energy uncoupling and membrane filtration processes represents an effective alternative method for simultaneous sludge reduction and sludge supernatant recovery.

关键词： Sludge reduction     Energy uncoupling     Ultrafiltration membrane     Membrane fouling    

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Studies of ultrasound disintegration of residual sludge and its energy consumption in water treatment of petrochemical plant

SHEN Jinfeng, YIN Xuan, GU Heping, Lv Xiaoping

《化学科学与工程前沿（英文）》 2007年 第1卷 第4期   页码 395-398 doi: 10.1007/s11705-007-0072-y

摘要： To investigate the influence of ultrasound pretreatment on sludge anaerobic digestion, the ultrasound disintegration of residual sludge in water treatment of petrochemical plant was studied, and the mechanisms of ultrasound and medium were introduced. Experimental results indicate that ultrasound cavitation induces the rise of sludge temperature, which improves ultrasound disintegration on sludge. U1trasound pretreatment can advance observably the quantity of chemical oxygen demand in sludge supernatant fluid (SCOD), which increases with ultrasound intensity and sonication time. The degree of ultrasound disintegration increases with the specific energy input. When the specific energy input is l0 000 kJ/kg of total dry solids, the degree of ultrasonic sludge disintegration reaches 40%.

关键词： specific     supernatant     petrochemical     ultrasound intensity     temperature    

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Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression variation of VEGF

ZHAN Rong, YU Qinghong, HUANG Haobo

《医学前沿（英文）》 2008年 第2卷 第4期   页码 356-360 doi: 10.1007/s11684-008-0068-0

摘要： The aim of this article is to explore the effect of arsenic trioxide (AsO) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expression variation of vascular endothelial growth factor (VEGF). The viability and apoptosis of U266 cells were observed by methylthiazolyl- tetrazolium (MTT) assay and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). The effect of AsO on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. We found that AsO could significantly inhibit the growth of U266 cells, and the concentration for 50% growth inhibition (IC) was 2 ?mol/L. After treatment with 2, 5, 10 ?mol/L AsO for 36 hours, dose-dependent apoptosis of U266 cells was observed. After treatment with 2, 5, 10 ?mol/L AsO for 72 hours, a dose-dependent reduction of VEGF in the supernatant of U266 cells culture was found. As far as single cells are concerned, nevertheless, the expression of VEGF mRNA did not vary. So we draw the conclusion that AsO could induce the apoptosis of U266 cells and inhibit their proliferation, decrease the tumor load, and lead to the reduction of VEGF in the culture supernatant, but not change the expression of VEGF in single U266 cells.

关键词： VEGF expression     expression variation     culture supernatant     labeling     concentration    

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Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

《医学前沿（英文）》 2008年 第2卷 第1期   页码 95-99 doi: 10.1007/s11684-008-0017-y

摘要： Schistosomiasis japonica, a zoonosis caused by , is endemic to the Philippines and China. Several vaccine candidates have been identified and tested in different animal models, but it is still unclear which will be optimal for testing in the field. Therefore, new antigens and strategies are necessary for vaccine development against schistosomiasis japonica. The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZ?-B and transformed into X-33 by electroporation. Three transformants were induced with methanol. The cultural supernatant was collected and tested by SDS-PAGE and Western blotting. The protein of rSj14-3-3 was prepared and purified and BALB/c mice were immunized which was followed by a challenging infection. The immuno-protection was then evaluated. The Sj14-3-3 gene was expressed and secreted into the medium and its molecular weight was about 35000 as determined by SDS-PAGE. Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity. The results of the immuno-protective experiments revealed that the worm reduction was 26.0%, 32.2%, and 36.8%, respectively. The number of eggs in liver tissue was reduced by 36.8%, 43.2%, and 46.1%, respectively. The recombinant Sj14-3-3 of eukaryotic expression in was successfully harvested. The molecular vaccine of Sj14-3-3 could partially induce resistance to the infection with in BALB/c mice. The recombinant protein Sj14-3-3 has promising immunological potentials for further approach to the diagnosis and development of molecular vaccine.

关键词： development     challenging     rSj14-3-3     resistance     cultural supernatant    

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标题 作者 时间 类型 操作

Co-application of energy uncoupling and ultrafiltration in sludge treatment: Evaluations of sludge reduction, supernatant

An Ding, Yingxue Zhao, Zhongsen Yan, Langming Bai, Haiyang Yang, Heng Liang, Guibai Li, Nanqi Ren

期刊论文

Studies of ultrasound disintegration of residual sludge and its energy consumption in water treatment of petrochemical plant

SHEN Jinfeng, YIN Xuan, GU Heping, Lv Xiaoping

期刊论文

Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression variation of VEGF

ZHAN Rong, YU Qinghong, HUANG Haobo

期刊论文

Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

期刊论文