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中医方证代谢组学——中药效应评价的有效途径 Review
张爱华, 孙晖, 闫广利, 韩莹, 赵琦琦, 王喜军
《工程(英文)》 2019年 第5卷 第1期 页码 60-68 doi: 10.1016/j.eng.2018.11.008
有效性评价是发现中药药效物质基础、先导化合物和质量标志物的重要前提,因此急需建立一种生物学语言,将中药有效性科学地表达出来,进一步凸显中医药的实用价值。证候和方剂是中医药的重要组成部分,与中药有效性直接相关。我们以证候和方剂为研究对象,建立了科学评价中药有效性的创新方法学体系——中医方证代谢组学。它将中药血清药物化学理论与代谢组学技术有机整合,在解决证候生物标记物的基础上,建立方剂药效生物评价体系,发现并确认中药药效物质基础。该策略为提高中医理论和临床实践的科学价值提供了有力支持。本文概述了中医方证代谢组学的研究策略,利用该方法揭示临床常见中医证候生物标记物及开展相关方剂的有效性评价研究,着重阐述了中药药效物质基础及质量标记物的发现。
代谢组扩展生物学的“旁中心法则”——对理解基因组学-糖组学-代谢组学-表观基因组学互作的意义
Albert Stuart Reece
《工程(英文)》 2023年 第26卷 第7期 页码 16-16 doi: 10.1016/j.eng.2022.07.011
The central dogma of biology holds that the transcription of DNA into RNA and the translation of RNA into proteins forms the primary axis of biological activity [1]. Following major advances in the description of the complex glycan and lipid chains that are added onto these basic building blocks, the glycome and lipidome have recently been added to this doctrine as an exciting new extension named the ‘‘paracentral dogma” [2]. However, it has been pointed out that biological systems can include many layers, which are described in modern omics technology platforms relating to both cell-intrinsic and cell-extrinsic layers of control, including metabolomic, microbiomic, immunological, epigenomic, epitranscriptomic, proteomic and phosphoproteomic layers [3].
It is well known that stem and progenitor cells have a metabolism that is based on glycolysis and glutaminolysis [4]. Although this provides less energy to the cell than oxidative phosphorylation, it suffices for these cells’ needs, since such cells are generally relatively quiescent and normally suppress energy-intensive processes such as genome duplication and transcription. Moreover, it has been shown that the high intracellular lactate levels involved in such states not only inhibits the key gatekeeper enzymes of oxidative phosphorylation (i.e., pyruvate dehydrogenase and carnitine palmitoyl acyltransferase) but also actually covalently modifies them by lactylation in order to maintain this inhibited metabolic–epigenomic state [5]. In addition, intermediate metabolism and nutrients are the source of the very extensive library of post-translational modifications to DNA, RNA, and proteins, as well as supplying cellular energy for many of the required reactions. Hence, the metabolic state locks in and reinforces the epigenomic state, and the metabolome and epigenome thereby play mutually reinforcing roles. This self-reinforcing coordination explains why it is so difficult to generate induced pluripotent cells and is a contributory explanation for why the described protocols typically have such low cellular yields.
These concepts become even more important when it is considered that cancer cells are de-differentiated, similarly rely on glycolysis and glutaminolysis, and are similarly metabolically–epigenomically–genomically synchronized. The disruption of this metabolic system is a key focus of mechanistic cancer research.
These important considerations imply that the descriptive and predictive power of the newly described ‘‘paracentral dogma” of biology may be usefully and meaningfully extended by including the metabolome, along with the genome, transcriptome, proteome, glycome, and lipidome, to describe cell-intrinsic regulation—not only in terms of another omics analytical layer but also as a fully predictive and interactive partner in the symphonic-like multilayer coordination that evidently comprises cellular regulatory layering.
功能代谢组学揭示黄芪多糖通过2-羟基丁酸改善肥胖小鼠的脂质代谢 Article
李冰冰, 洪颖, 顾彧, 叶圣洁, 胡凯莉, 姚建, 丁侃, 赵爱华, 贾伟, 李后开
《工程(英文)》 2022年 第9卷 第2期 页码 111-122 doi: 10.1016/j.eng.2020.05.023
血浆代谢组学结合超微弱发光表征早期2型糖尿病的中医证型 Article
何敏, 孙濛濛, Slavik Koval, Roeland Van Wijk, Thomas Hankemeier, Jan Van der Greef, Eduard P.A. Van Wijk, 王梅
《工程(英文)》 2019年 第5卷 第5期 页码 916-923 doi: 10.1016/j.eng.2019.03.011
肝脏移植术后糖尿病患者肠道微生物组的变化 Article
凌琪, 韩玉秋, 马越, 王晓森, 朱铮, 王靖宇, 曹佳莹, 林笑含, 王军, 王保红
《工程(英文)》 2023年 第31卷 第12期 页码 98-111 doi: 10.1016/j.eng.2023.09.006
人类蛋白质N-糖基化的十二年全基因组关联研究 Review
Anna Timoshchuk, Sodbo Sharapov, Yurii S. Aulchenko
《工程(英文)》 2023年 第26卷 第7期 页码 17-31 doi: 10.1016/j.eng.2023.03.013
Most human-secreted and membrane-bound proteins have covalently attached oligosaccharide chains, or glycans. Glycosylation influences the physical and chemical properties of proteins, as well as their biological functions. Unsurprisingly, alterations in protein glycosylation have been implicated in a growing number of human diseases, and glycans are increasingly being considered as potential therapeutic targets, an essential part of therapeutics, and biomarkers. Although glycosylation pathways are biochemically well-studied, little is known about the networks of genes that guide the cell- and tissue-specific regulation of these biochemical reactions in humans in vivo. The lack of a detailed understanding of the mechanisms regulating glycome variation and linking the glycome to human health and disease is slowing progress in clinical applications of human glycobiology. Two of the tools that can provide much sought-after knowledge of human in vivo glycobiology are human genetics and genomics, which offer a powerful data-driven agnostic approach for dissecting the biology of complex traits. This review summarizes the current state of human populational glycogenomics. In Section 1, we provide a brief overview of the N-glycan's structural organization, and in Section 2, we give a description of the major blood plasma glycoproteins. Next, in Section 3, we summarize, systemize, and generalize the results from current N-glycosylation genome-wide association studies (GWASs) that provide novel knowledge of the genetic regulation of the populational variation of glycosylation. Until now, such studies have been limited to an analysis of the human blood plasma N-glycome and the N-glycosylation of immunoglobulin G and transferrin. While these three glycomes make up a rather limited set compared with the enormous multitude of glycomes of different tissues and glycoproteins, the study of these three does allow for powerful analysis and generalization. Finally, in Section 4, we turn to genes in the established loci, paying particular attention to genes with strong support in Section 5. At the end of the review, in Sections 6 and 7, we describe special cases of interest in light of new discoveries, focusing on possible mechanisms of action and biological targets of genetic variation that have been implicated in human protein N-glycosylation.
深古菌门的核心代谢功能和热环境起源 Article
冯晓远, 王寅炤, Rahul Zubi, 王风平
《工程(英文)》 2019年 第5卷 第3期 页码 498-504 doi: 10.1016/j.eng.2019.01.011
时间序列多组学整合分析揭示原代肝细胞体外培养去分化过程伴随非降解性泛素化修饰的增加 Article
姜正一, 孙泽宇, 欧阳晓希, 赵亚磊, 周梦豪, 王保红, 李启睿, 范林骁, 张赛男, 李兰娟
《工程(英文)》 2020年 第6卷 第11期 页码 1302-1314 doi: 10.1016/j.eng.2020.02.011
贾谦,陈永杰,陈光曼,杨巨平,应光荣
《中国工程科学》 2004年 第6卷 第7期 页码 4-13
中医学以“天人相应”、“阴阳平衡”为哲学基础,以“整体观念”、“辨证论治”为主要特点,以中药、针灸等为主要治疗手段来调整中医学所提倡的人与自然(包括细菌、病毒等)和谐共处,而不是对微生物大规模灭活的理念,维持了微生物与人类之间微妙的生理平衡,这正是医学未来的发展方向。千百年来,中医药为中华民族的繁衍昌盛做出了不可磨灭的贡献,使其在无数次瘟疫的侵袭下从未像欧洲一样一死上千万人;同时,中医药“简便廉验”,在预防和治疗慢性病、多因素复杂性疾病以及新型病毒性疾病等方面尤有所长建议实施“中医药五大振兴工程”,即中医药政策法规保障工程、中医药人才工程、中医药科研工程、乡村中医工程以及中医药行政管理体制改革工程,以做到中医药的可持续发展。
多组学联用揭示花粉过敏基于肠道菌的新机制 Article
韩珮, 李丽莎, 王子熹, 锡琳, 于航, 丛林, 张正威, 符洁, 彭冉, 潘利斌, 马殊荣, 王学艳, 王洪田, 王向东, 王琰, 孙劲旅, 蒋建东
《工程(英文)》 2022年 第15卷 第8期 页码 115-125 doi: 10.1016/j.eng.2021.03.013
朱汉章
《中国工程科学》 2006年 第8卷 第7期 页码 1-15
针刀医学是在中医基本理论指导下,吸收现代科技成果,包括西医学的新成果,再创造而形成的新的医学理论体系;经过30年的发展,形成了一套完整的理论体系,是中医在基本理论方面实现现代化的成功范例之一;包括四大基础理论和六大组成部分:四大基础理论是闭合性手术理论,慢性软组织损伤的病因病理学理论,骨质增生新的病因学理论,脊柱区带病因学及人体存在庞大的电生理线路的理论;六大组成部分是针刀医学病理生理学,针刀医学影像学,针刀医学手法学,针刀医学诊断学,针刀医学治疗学和针刀医学护理学;经过临床实践和深入研究,其理论不断深化,治疗技术逐渐提高,适应症范围不断扩大,形成了一门独特的医学新学科,为解决当今医学上的各种难题开辟了一条新路,在临床研究和实验研究方面取得了多项成果
温维亮,郭新宇 ,张颖,顾生浩,赵春江
《中国工程科学》 2023年 第25卷 第4期 页码 227-238 doi: 10.15302/J-SSCAE-2023.04.015
标题 作者 时间 类型 操作
血浆代谢组学结合超微弱发光表征早期2型糖尿病的中医证型
何敏, 孙濛濛, Slavik Koval, Roeland Van Wijk, Thomas Hankemeier, Jan Van der Greef, Eduard P.A. Van Wijk, 王梅
期刊论文
多组学联用揭示花粉过敏基于肠道菌的新机制
韩珮, 李丽莎, 王子熹, 锡琳, 于航, 丛林, 张正威, 符洁, 彭冉, 潘利斌, 马殊荣, 王学艳, 王洪田, 王向东, 王琰, 孙劲旅, 蒋建东
期刊论文
分子标记的开发和系统发育基因组学实操班
2019年06月27日
会议信息