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Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spot14 and Cyp2e1 expression

Xiaofang Cui, Benting Ma, Yan Wang, Yan Chen, Chunling Shen, Ying Kuang, Jian Fei, Lungen Lu, Zhugang Wang

《医学前沿（英文）》 2019年第13卷第1期页码 104-111 doi: 10.1007/s11684-017-0568-x

摘要： Mitochondrion-localized retinol dehydrogenase 13 (Rdh13) is a short-chain dehydrogenase/reductase involved in vitamin A metabolism in both humans and mice. We previously generated knockout mice and showed that Rdh13 deficiency causes severe acute retinal light damage. In this study, considering that Rdh13 is highly expressed in mouse liver, we further evaluated the potential effect of Rdh13 on liver injury induced by carbon tetrachloride (CCl ). Although Rdh13 deficiency showed no significant effect on liver histology and physiological functions under regular culture, the mice displayed an attenuated response to CCl -induced liver injury. Their livers also exhibited less histological changes and contained lower levels of liver-related metabolism enzymes compared with the livers of wild-type (WT) mice. Furthermore, the mice had Rdh13 deficiency and thus their liver cells were protected from apoptosis, and the quantity of their proliferative cells became lower than that in WT after CCl exposure. The ablation of gene decreased the expression levels of thyroid hormone-inducible nuclear protein 14 (Spot14) and cytochrome P450 (Cyp2e1) in the liver, especially after CCl treatment for 48 h. These data suggested that the alleviated liver damage induced by CCl in mice was caused by Cyp2e1 enzymes, which promoted reductive CCl metabolism by altering the status of thyroxine metabolism. This result further implicated Rdh13 as a potential drug target in preventing chemically induced liver injury.

关键词： retinol dehydrogenase 13 carbon tetrachloride acute liver injury Cyp2e1 Spot14

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利用cyp17a1缺失的伪雄鲤成功构建全雌鲤群体 Article

翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战

《工程（英文）》 2022年第8卷第1期页码 181-189 doi: 10.1016/j.eng.2021.03.026

摘要：本研究针对雌鲤生长快于雄鲤的两性生长异形的特性，以cyp17a1 为靶点进行了CRISPR/Cas9介导的基因编辑操作，利用cyp17a1 敲除后形成的伪雄鲤，成功构建了全雌鲤群体首先，我们发现无论遗传性别型为XX还是XY，其cyp17a1 缺失鲤个体性腺均发育为精巢，并能正常产精。其次，利用确定的雄性特异DNA分子标记，筛选到cyp17a1 ^-/- XX伪雄个体，其具有正常的精巢发育和精子发生。将全雌群体和对照组雌雄混合群体各500 尾进行同塘生长对比养殖，至8 月龄或12 月龄时随机抽样检测，发现cyp17a1杂合突变雌鲤体重比同塘性别混养对照组分别高6.60%或32.66%

关键词：鲤两性异形生长 cyp17a1 性类固醇激素全雌群体

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年第8卷第2期

摘要：

Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that MdSIZ1 in apple has a negative relationship with drought tolerance. MdSIZ1 RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H₂O₂ and malondialdehyde contents, and higher catalase activity. In addition, MdSIZ1 RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

关键词： apple / drought tolerance / gene expression / MdSIZ1

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年第8卷第4期页码 662-662 doi: 10.15302/J-FASE-2021408

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Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure treatment

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

《农业科学与工程前沿（英文）》 2017年第4卷第2期页码 208-219 doi: 10.15302/J-FASE-2017138

摘要： Most animals, including humans, produce natural sex hormones such as estrogens: 17β-estradiol (E2) and estrone (E1). These compounds are able to disrupt the reproductive systems of living organisms at trace concentrations (ng·L ). This experiment tests the hypothesis that 1% slow pyrolysis biochar-amended sandy soil could retain significant amount of estrogens (E1, E2) from poultry manure in its second year of application. The experiment was conducted over 46 days and consisted of a series of lysimeters containing sandy soil with biochar-amended topsoil. The application rate of poultry manure was kept at 2.47 kg·m . The biochar held a significant concentration of hormone during the first year of its application. However, in the following year (current study), there was no significant retention of hormones in the biochar-amended soil. During the first year after application, the biochar was fresh, so its pores were available for hydrophobic interactions and held significant concentration of hormones. As time passed there were several biotic and abiotic changes on the surface of the biochar so that after some physical fragmentation, pores on the surface were no longer available for hydrophobic interactions. The biochar started releasing dissolved organic carbon, which facilitated greater mobility of hormones from poultry manure down the soil profile.

关键词： adsorption degradation dissolved organic carbon 17β-estradiol (E2) estrone (E1)

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

《农业科学与工程前沿（英文）》页码 247-261 doi: 10.15302/J-FASE-2021388

摘要： Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that in apple has a negative relationship with drought tolerance. RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H O and malondialdehyde contents, and higher catalase activity. In addition, RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

关键词： apple drought tolerance gene expression MdSIZ1

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Effect of atorvastatin on tumor growth and metastasis in a breast cancer cell xenograft model and its mechanism

Liu LIU MD, PhD, Yaogui NING MM, Chen CHEN MD, Daowen WANG MD, PhD,

《医学前沿（英文）》 2009年第3卷第4期页码 443-446 doi: 10.1007/s11684-009-0079-5

摘要： This paper aims to evaluate the effects and the possible mechanisms of atorvastatin on tumor growth and metastasis in a xenograft tumor model. Twenty-four female athymic BALB/C mice with MDA-MB-435 xenograft tumors were randomly assigned to three groups: a control group, a low-dose atorvastatin treatment group, and a high-dose atorvastatin treatment group. The mice in the treatment groups began to be administered with atorvastatin (10 or 20 mg/kg per day) when the xenograft tumors reached 1 cm in diameter. At the end of the experiment, the tumor volume and weight and the lung metastasis colonies of each mouse were measured. Western blotting was applied to detect phosphorylation of protein kinase B (PKB, Akt), extracellular signal regulated kinase (ERK), c-Jun N-terminal Kinase (JNK), and the expression of cytochrome P450 (CYP) subtype CYP2J2. Atorvastatin suppressed xenograft tumor growth and metastasis both in the low-dose and the high-dose treatment groups ( < 0.05). Atorvastatin also decreased the phosphorylated Akt (p-Akt) and p-ERK but increased p-JNK expression. However, atorvastatin did not alter the expression of CYP2J2 in tumor tissue. This suggests that atorvastatin has the efficacy of suppressing tumor growth and metastasis . These effects were not dependent on down-regulation of CYP2J2 expression.

关键词： atorvastatin xenograft tumor metastasis CYP2J2

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Heterologous expression of

Potter,Francis E. Nano

《环境科学与工程前沿（英文）》 2017年第11卷第2期 doi: 10.1007/s11783-017-0910-1

摘要： Maximum growth rate of mutant was 0.083 h with 5% CO . Maximum biomass concentration of mutant was 3.697 g·L . mutant can tolerate gas aeration with 15% CO . Maximum specific activity of laminarinase was 4.325 U·mg dry mass. Optimal pH and temperature of laminarinase activity were 8.0 and 70°C. The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a pH of 8.0 and a temperature of 70°C. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h , a biomass productivity of 0.42 g·L ·d , a biomass concentration of 3.697 g·L , and a specific enzyme activity of the mutant strain of 4.325 U·mg dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still grew at 15% CO2 concentration, as reflected by the biomass productivity (0.26 g·L ·d ), biomass concentration (2.416 g·L ), and specific enzyme activity (3.247 U·mg dry mass).

关键词： Synechococcus sp. PCC 7002 Thermotoga maritima LamA gene Endo-β-1 3-glucanase CO₂ fixation

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Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

null

《医学前沿（英文）》 2016年第10卷第1期页码 76-84 doi: 10.1007/s11684-015-0426-7

摘要：

Anti-β₂ glycoprotein I (anti-β₂GP I ) antibodies are important contributors to thrombosis, especially in patients with antiphospholipid syndrome (APS). However, the mechanism by which anti-β₂GP I antibodies are involved in the pathogenesis of thrombosis is not fully understood. In this report, we investigated the role of anti-β₂GP I antibodies in complexes with β₂GP I as mediators of platelet activation, which can serve as a potential source contributing to thrombosis. We examined the involvement of the apolipoprotein E receptor 2' (apoER2') and glycoprotein I ba (GP I bα) in platelet activation induced by the anti-β₂GP I /β₂GP I complex. The interaction between the anti-β₂GP I /β₂GP I complex and platelets was examined using in vitro methods, in which the Fc portion of the antibody was immobilized using protein A coated onto a microtiter plate. Platelet activation was assessed by measuring GP II b/ III a activation and P-selectin expression and thromboxane B₂ production as well as p38 mitogen-activated protein kinase phosphorylation. Our results revealed that the anti-β₂GP I /β₂GP I complex was able to activate platelets, and this activation was inhibited by either the anti-GP I bα antibody or the apoER2' inhibitor. Results showed that the anti-β₂GP I /β₂GP I complex induced platelet activation via GP I bα and apoER2', which may then contribute to the prothrombotic tendency in APS patients.

关键词： anti-β2GP I /β2GP I complex platelet GP I bα apoER2' thrombosis

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基于半导体纳米晶体的神经突触器件 Review

步明轩1,2,王越1,2,尹蕾1,2,童周禹1,2,张懿强3,杨德仁1,2,4,5,皮孝东1,2,4,5

《信息与电子工程前沿（英文）》 2022年第23卷第11期页码 1579-1601 doi: 10.1631/FITEE.2100551

摘要：

近年来，人们对信息处理的需求日益增长，脑启发式神经形态器件得到了广泛的关注。突触器件作为一类重要的神经形态器件，在短短几年内迅速升温。在用于制备突触器件的各种材料中，半导体纳米晶体（NCs）因其优异的电学和光学性能而成为首选材料之一。本综述论文首先介绍了基于半导体纳米晶体的突触器件的研究背景及半导体纳米晶体的基本性质。然后，根据器件有源层所用纳米晶体种类的不同，分类介绍了基于纳米晶体的突触器件的最新研究进展。最后，讨论了基于半导体纳米晶体的突触器件目前仍面临的问题和挑战。

关键词：半导体纳米晶体；突触器件；神经形态计算

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Primary hypertrophic osteoarthropathy: an update

null

《医学前沿（英文）》 2013年第7卷第1期页码 60-64 doi: 10.1007/s11684-013-0246-6

摘要：

Digital clubbing, which has been recognized as a sign of systemic disease, is one of the most ancient diseases. However, the pathogenesis of clubbing and hypertrophic osteoarthropathy has hitherto been poorly understood. The study of a clinically indistinguishable idiopathic form (primary hypertrophic osteoarthropathy, PHO) provides an opportunity to understand the pathogenesis of hypertrophic osteoarthropathy. Current advances in the study of PHO are discussed. The impaired metabolism of prostaglandin E2 (PGE2) plays a central role in its pathogenesis.

关键词： digital clubbing primary hypertrophic osteoarthropathy prostaglandin E2 (PGE2)

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面向语义通信的模分多址技术 Research Article

张平1,2,3,许晓东1,2,3,董辰1,牛凯1,2,梁灏泰1,梁子键1,秦晓琦1,孙梦颖1,陈昊2,马楠1,2,许文俊1,王光宇1,陶小峰2,4

《信息与电子工程前沿（英文）》 2023年第24卷第6期页码 801-812 doi: 10.1631/FITEE.2300196

摘要：在多用户系统中，系统资源应分配给不同用户。在传统通信系统中，系统资源通常包括时间、频率、空间和功率，因此广泛使用诸如时分多址（TDMA）、频分多址（FDMA）、空分多址（SDMA）、码分多址（CDMA）、非正交多址（NOMA）之类多址技术。在被认为是下一代通信系统新范式的语义通信中，我们从语义角度，以基于模型的人工智能方法，从信源中提取高维语义域特征，并针对信源和信道特征联合构建模型信息空间。从模型信息空间中挖掘语义信息的共性化和个性化信息，提出一种新的基于语义域资源的多址技术，称为模分多址（MDMA）。从信息论角度，证明模分多址比传统多址技术获得更多性能提升。仿真结果表明，模分多址比传统多址技术节省更多带宽资源，并且在低信噪比条件下，在加性高斯白噪声（AWGN）信道中，相比非正交多址具有至少5 dB的性能优势。

关键词：模分多址（MDMA）；语义通信；多址技术

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有限域上Galois型非线性移位寄存器的能观性 Research Article

高哲1,冯俊娥1,于永渊1,崔彦君2

《信息与电子工程前沿（英文）》 2022年第23卷第10期页码 1533-1545 doi: 10.1631/FITEE.2200228

摘要：

能观性可以确保任何两个不同初始状态都可以由它们的输出序列唯一确定，因此流密码必须避免不可观的非线性反馈移位寄存器，以防止等效密钥的出现。本文讨论了有限域上Galois型非线性反馈移位寄存器的能观性。通过半张量积，Galois型非线性反馈移位寄存器可被视为逻辑网络。本文介绍了状态转移矩阵的向量形式，据此提出一个充分必要条件以及判定一般Galois型非线性反馈移位寄存器能观性的算法。此外，本文定义了一个新的能观性矩阵，通过该矩阵可推导出计算复杂度较低的矩阵方法。此外，研究两种特殊类型的Galois型非线性反馈移位寄存器的能观性：全长Galois型非线性反馈移位寄存器和非奇异Galois型非线性反馈移位寄存器。提出两种方法确定这两种特殊类型的非线性反馈移位寄存器的能观性，并提供一些数值示例支持这些结果。

关键词：能观性；非线性反馈移位寄存器（NFSRs）；Galois型非线性反馈移位寄存器；半张量积；有限域；逻辑网络

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基于玻璃通孔的Ka波段宽带滤波封装天线 Research Article

方针1,2,张继华1,2,3,高莉彬1,2,陈宏伟1,2,李文磊1,2,梁天鹏1,2,蔡旭东1,2,蔡星周3,贾惟聪3,郭欢3,李勇3

《信息与电子工程前沿（英文）》 2023年第24卷第6期页码 916-926 doi: 10.1631/FITEE.2200573

摘要： BPF单元有3对λg/4槽(缺陷微带结构)和两对λg/2 U形缝隙(缺陷地结构)。该天线实现了大带宽和高辐射效率，这可能与玻璃基板的叠加和玻璃通孔馈电有关。

关键词：滤波封装天线；玻璃通孔；三维封装器件；激光键合

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Microwave-assisted synthesis and antimicrobial activities of 2-aryl-3-(naphthalene-1 or 2-yl)-1,3-thiazolidin

Hua CHEN, Yanan LI, Jie BAI, Lian ZHAO, Xiangguo YUAN, Xiaoliu LI, Keqiang CAO

《化学科学与工程前沿（英文）》 2009年第3卷第2期页码 186-191 doi: 10.1007/s11705-009-0004-0

摘要： 2-aryl-3-(naphthalene-1 or 2-yl)-1,3-thiazolidin-4-ones and were synthesized in 41%-67% yield by using microwave-assisted one-pot protocol. The structures of the new compounds , , , , , , and - were confirmed by IR, NMR, MS, and elemental analysis. The antimicrobial activities of the compounds against pv. lachrymans (Smith et Bryan) Young, Dye & Wilkie, Pers., and Blum. were examined. Some of the compounds showed good antifungical activity against Blum.

关键词： microwave-assisted synthesis naphthyl thiazolidin-4-one antimicrobial

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标题作者时间类型操作

Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spot14 and Cyp2e1 expression

Xiaofang Cui, Benting Ma, Yan Wang, Yan Chen, Chunling Shen, Ying Kuang, Jian Fei, Lungen Lu, Zhugang Wang

期刊论文

利用cyp17a1缺失的伪雄鲤成功构建全雌鲤群体

翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure treatment

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

期刊论文

Effect of atorvastatin on tumor growth and metastasis in a breast cancer cell xenograft model and its mechanism

Liu LIU MD, PhD, Yaogui NING MM, Chen CHEN MD, Daowen WANG MD, PhD,

期刊论文

Heterologous expression of

Potter,Francis E. Nano

期刊论文

Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

null

期刊论文

基于半导体纳米晶体的神经突触器件

步明轩1,2,王越1,2,尹蕾1,2,童周禹1,2,张懿强3,杨德仁1,2,4,5,皮孝东1,2,4,5