资源类型

期刊论文 7

年份

2023 1

2021 1

2018 1

2014 3

2001 1

关键词

光流法 1

卵母细胞 1

卵母细胞去核 1

去核 1

小鼠 1

机器人化克隆 1

细胞内应变 1

细胞内损伤 1

表面张力辅助 1

展开 ︾

检索范围：

排序： 展示方式：

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 185-190 doi: 10.15302/J-FASE-2014035

摘要： DNA damage is one of the most common threats to meiotic cells. It has the potential to induce infertility and genetic abnormalities that may be passed to the embryo. Here, we reviewed exogenous factors which could induce DNA damage. Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic development. Complex DNA damage, double-strand breaks, represents a more difficult repair process and involves various repair pathways. Understanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for ovarian cancer and fertility preservation.

关键词： DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

HTML PDF 收藏

表面张力辅助去核法对小鼠卵母细胞的去核研究

孟庆刚,朱士恩,曾申明,张忠诚

《中国工程科学》 2001年 第3卷 第11期   页码 65-69

摘要：

论述了一种新的小鼠卵母细胞去核方法一一表面张力辅助去核法（STA),并同时与其他3种去核方 法（简称A法、B法、C法）进行比较。STA法是将卵母细胞核在固定管的辅助下，借助液体表面张力通过透 明带上的破口被挤出。经STA法去核的卵母细胞质体用于电融合介导的小鼠耳成纤维细胞核移植实验，结果有 76.1 %细胞与细胞质体融合形成重组胚，85.4 %的重组胚形成原核，49.4%卵裂形成2-细胞。

关键词： 表面张力辅助     去核     卵母细胞     小鼠    

HTML PDF 收藏

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

《医学前沿（英文）》 2018年 第12卷 第5期   页码 518-524 doi: 10.1007/s11684-017-0575-y

摘要：

The increased levels of intracellular reactive oxygen species (ROS) in granulosa cells (GCs) may affect the pregnancy results in women with polycystic ovary syndrome (PCOS). In this study, we compared thein vitro fertilization and embryo transfer (IVF-ET) results of 22 patients with PCOS and 25 patients with tubal factor infertility and detected the ROS levels in the GCs of these two groups. Results showed that the PCOS group had significantly larger follicles on the administration day for human chorionic gonadotropin than the tubal factor group (P<0.05); however, the number of retrieved oocytes was not significantly different between the two groups (P>0.05). PCOS group had slightly lower fertilization, cleavage, grade I/II embryo, clinical pregnancy, and implantation rates and higher miscarriage rate than the tubal factor group (P>0.05). We further found a significantly higher ROS level of GCs in the PCOS group than in the tubal factor group (P<0.05). The increased ROS levels in GCs caused GC apoptosis, whereas NADPH oxidase 2 (NOX2) specific inhibitors (diphenyleneiodonium and apocynin) significantly reduced the ROS production in the PCOS group. In conclusion, the increased ROS expression levels in PCOS GCs greatly induced cell apoptosis, which further affected the oocyte quality and reduced the positive IVF-ET pregnancy results of women with PCOS. NADPH oxidase pathway may be involved in the mechanism of ROS production in GCs of women with PCOS.

关键词： PCOS     ROS     granulosa cell     IVF-ET     NADPH oxidase    

HTML PDF 收藏

基于细胞内应变评估的卵母细胞去核方法及其在机器人化克隆中的应用 Article

孙明竹, 刘曜玮, 崔茂盛, 赵启立, 赵相飞, 张艺荻, 黄晶晶, 卢桂章, 赵新

《工程（英文）》 2023年 第24卷 第5期   页码 73-83 doi: 10.1016/j.eng.2022.04.016

摘要：

自1996 年第一只克隆羊诞生，克隆技术由于在动物育种方面的巨大潜力而得到了很大关注。体细胞核移植技术是动物克隆的核心，其操作过程非常复杂，不可避免地造成细胞内损伤。通常来说，只有不到1%的重构胚胎能够发育成克隆动物，克隆的低成功率是制约其广泛应用的主要原因之一。为此，本文提
出了一种基于细胞内应变评估的卵母细胞去核方法，以减少体细胞核移植中的潜在细胞内损伤，从而提高克隆成功率。首先，根据细胞内速度场计算细胞内应变，进而将内应变作为评价标准来改进去核操作；在此基础上，开发了一套机器人批量体细胞核移植系统，用于实现机器人化克隆。实验结果表明，本文方法将体细胞核移植后的胚胎囊胚率从10.0%提高到20.8%，并成功获得17 头克隆小猪。这是世界上首批由机器人完成克隆操作获得的克隆动物。对比手动核移植操作，本文将克隆成功率从平均0.73%提升到了2.50%，大幅提升了克隆效率。与此同时，基于细胞内应变的去核方法有望应用于其他生物操作，并建立一种通用的减少细胞内损伤的细胞操作规范。

关键词： 卵母细胞去核     机器人化克隆     细胞内应变     细胞内损伤     光流法    

HTML PDF 收藏

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Fengxia YIN,Hui LIU,Shorgan BOU,Guangpeng LI

《农业科学与工程前沿（英文）》 2014年 第1卷 第2期   页码 104-113 doi: 10.15302/J-FASE-2014003

摘要： Oocytes are unique cells with the inherent capability to reprogram nuclei. The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer. The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming. The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin. The oocyte first obtains its reprogramming capability in the early fetal follicle, and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes. The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming. Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells. The maternal pronucleus exerts a predominant, transcription-dependent effect on embryo cytofragmentation, with a lesser effect imposed by the ooplasm and the paternal pronucleus. With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.

关键词： nuclear reprogramming     somatic cell     transcription factors     transcriptomics    

HTML PDF 收藏

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 314-320 doi: 10.15302/J-FASE-2014038

摘要： This study was conducted to systematically assess the reproductive performance of transgenic ewes. In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the transgene did not affect the reproductive performance in sheep.

关键词： TLR4 transgenic ewe     safety assessment     reproductive trait     oocyte     DNA methylation    

HTML PDF 收藏

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

《医学前沿（英文）》 2021年 第15卷 第1期   页码 144-154 doi: 10.1007/s11684-020-0792-7

摘要： Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.

关键词： human metaphase II oocyte     vitrification     cryostorage duration     single-cell RNA-Seq     lncRNA    

HTML PDF 收藏

标题 作者 时间 类型 操作

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

期刊论文

表面张力辅助去核法对小鼠卵母细胞的去核研究

孟庆刚,朱士恩,曾申明,张忠诚

期刊论文

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

期刊论文

基于细胞内应变评估的卵母细胞去核方法及其在机器人化克隆中的应用

孙明竹, 刘曜玮, 崔茂盛, 赵启立, 赵相飞, 张艺荻, 黄晶晶, 卢桂章, 赵新

期刊论文

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Fengxia YIN,Hui LIU,Shorgan BOU,Guangpeng LI

期刊论文

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

期刊论文

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

期刊论文