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期刊论文 11

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产业化 1

免疫抑制 1

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施马伦贝格病毒 1

牛疱疹病毒1型 1

牛疱疹病毒4型 1

牛病毒性腹泻病毒 1

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Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo

null

《医学前沿（英文）》 2017年 第11卷 第2期   页码 247-252 doi: 10.1007/s11684-017-0519-6

摘要：

The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.

关键词： in vitro fertilization     PCOS     free radical     embryo quality    

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Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo

null

《医学前沿（英文）》 2017年 第11卷 第4期   页码 563-569 doi: 10.1007/s11684-017-0535-6

摘要：

The superiority of the cumulative outcomes of day 5/6 embryo transfer to those of day 2/3 embryo transfer in infertile couples has been debated. This retrospective study included data collected from 1051 patients from July 2011 to June 2014. Multiple maternal baseline covariates were subjected to propensity score matching analysis, and each day 5/6 group woman was matched to one day 2/3 group woman. A systematic meta-analysis was conducted to validate the results. After matching was completed, 217 patients on the day 2/3 group were matched with those on the day 5/6 group, and no significant differences in the baseline characteristics were observed between the two groups. The cumulative pregnancy rate (57.14% vs. 53.46%, OR 1.16, 95% CI 0.79–1.70) and cumulative live birth rate (53.00% vs. 49.77%, OR 1.14, 95% CI 0.78–1.66) of day 5/6 embryo transfers were higher than those of day 2/3 embryo transfers, but this difference was not significant. The mean cycles per live birth and mean days per live birth in the day 5/6 group were significantly lower than those in the day 2/3 group. This study demonstrated that day 5/6 embryo transfer is a more cost-effective and time-efficient policy than day 2/3 embryo transfer to produce a live baby.

关键词： blastocyst     embryo transfer     cumulative pregnancy rate     cumulative live birth rate     IVF    

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Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

《农业科学与工程前沿（英文）》 2020年 第7卷 第2期   页码 148-160 doi: 10.15302/J-FASE-2019305

摘要：

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.

关键词： animal breeding     cattle     cloning     CRISPR/Cas9     cytoplasmic injection     embryo     genome editing     germline chimaeras     HDR     livestock improvement     TALENs    

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加速胚胎移植技术产业化促进我国养牛业发展

郭志勤

《中国工程科学》 2000年 第2卷 第3期   页码 12-17

摘要：

从我国养牛业的现状和存在的主要问题，指出养牛业需要大力发展，当前必须大量引种和繁殖种牛。 而胚胎移植是引种的一种最好手段，具有方法简便、成本低、缩短改良时间、减少疾病传播等优点。同时，进 一步分析了我国胚胎移植技术的研究概况，阐明了胚胎移植的主要技术关键及产业化的必要性和可能性。并根 据我国养牛业的现状，提出奶牛、肉牛业应用胚胎移植技术的措施与办法。

关键词： 胚胎移植     产业化     养牛业    

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Factors affecting early embryonic development in cattle: relevance for bovine cloning

Yanna DANG, Kun ZHANG

《农业科学与工程前沿（英文）》 2019年 第6卷 第1期   页码 33-41 doi: 10.15302/J-FASE-2018228

摘要：

Female infertility represents a major challenge for improving the production efficiency in the dairy industry. Historically, fertility has declined whereas milk yield has increased tremendously due to intensive genetic selection. evidence reveals about 60% pregnancy loss takes place during the first month following fertilization. Meanwhile, early embryo development is significant for somatic cell nuclear transfer in cattle as a large proportion of cloned embryos fail to develop beyond peri-implantation stage. Oocyte quality is of utmost importance for the early embryo to develop to term for both fertilized and cloned embryos. Epigenetic reprogramming is a key process occurring after fertilization and critical roles of epigenetic modifiers during preimplantation development are now clear. Incomplete epigenetic reprogramming is believed to be a major limitation to cloning efficiency. Treatment of cloned embryos with epigenetic modifying drugs (e.g., Trichostatin A) could greatly improve cloning efficiency in both mice and cattle. Recently, the rapid progress in high-throughput sequencing technologies has enabled detailed deciphering of the molecular mechanisms underlying these events. The robust efficiency of genomic editing tools also presents an alternative approach to the functional annotation of genes critical to early development.

关键词： bovine cloning     embryo development     somatic cell nuclear transfer     X-inactive specific transcript    

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Reproductive toxicity study with a novel deoxyguanosine analogue (Metacavir) in pregnant SD rats

null

《医学前沿（英文）》 2015年 第9卷 第1期   页码 82-89 doi: 10.1007/s11684-015-0376-0

摘要：

Our preliminary studies demonstrated that Metacavir has potential to become a new anti-HBV agent. The main targets of the toxic effects of Metacavir, in rhesus monkeys, were gastrointestinal tracts, liver, blood, and kidneys, which were not related to mitochondrial effects. In this study, the maternal toxicity, embryo-fetal developmental toxicity and teratogenicity were studied in pregnant Sprague-Dawley rats after intragastric administration of Metacavir (200, 100, 50, 0 mg/kg body weight) during the first 6–15 days of pregnancy. Slower weight gain was observed in 5 out of 21 rats subjected to a 200 mg/kg dose, as well as 2 out of 20 subjected to a 100 mg/kg dose. Compared with the solvent control group, the calibration weight gain in the 200 mg/kg and 100 mg/kg dosage groups respectively, during first 6–20 pregnant days were significantly different (P<0.01, P<0.05). Significant dose related adverse effects to other reproductive parameters were not seen in F0 and F1, but the number of stillbirths in high dose group showed notably difference compared with the control group (P<0.05), while the litter incidence showed no difference. No Metacavir-associated pathological changes were observed. The present research indicated that at a dose of 200 mg/(kg·d) (i.e., 40 times the effective dose in rats), Metacavir shows some maternal toxicity to SD rats. The embryotoxicity in the 200 mg/kg group encompass decreased fetal body weight, and higher fetal mortality rates, compared with the control group. However, the litter incidence showed no statistical difference. All the treated rats displayed normal bone development, no teratogenicity and without adverse effects on fetal development, thus indicating that below a dose of 200 mg/(kg·d) there is no teratogenic side effects.

关键词： deoxyguanosine analogue     Metacavir     pregnancy     maternal toxicity     embryo toxicity     teratogenicity    

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Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface epithelium in mice

Linlin LIANG, Bei XU, Guijin ZHU

《医学前沿（英文）》 2009年 第3卷 第2期   页码 220-226 doi: 10.1007/s11684-009-0037-2

摘要： This study aimed to evaluate the effect of repeated ovarian stimulation (OS) on the ovarian follicular population and morphology in female mice and its influence on the embryo’s developmental ability, and the profile of the ovarian surface epithelium (OSE). A total of 75 mice were enrolled in this experiment and randomly assigned into three groups: repeated ovarian stimulated group [ =25; receiving 5 IU pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotropin (hCG) at 6 day intervals for 5 cycles]; single ovarian stimulated group ( =25; receiving 5 IU PMSG and hCG for 1 cycle), and control group ( =25; without additional treatment). The follicle number at various stages and the morphologies were recorded respectively in the three groups. The harvested oocytes or embryos, cleavage rate, good quality embryo rate, and blastocyst production rate were counted and calculated, and the proliferations of ovarian surface epithelium were evaluated respectively. In the three groups, the single ovarian stimulation treatment significantly increased the mean number of ovarian oocytes or embryos (39.25±10.77 one-cell embryos/female); on the other hand, repeated gonadotropin stimulation obtained the lowest mean number (5.15± 2.81 eggs/female, <0.01). Repeated ovarian stimulation also tended to decrease normal follicles of primary follicles (66.67%) and secondary follicles (72.86%), and got the lowest cleavage rate (67.47%), lowest good quality embryo rate (2.41%), and lowest blastocyst production rate (0). The OSE cells adjacent to the antral follicles and corpus luteum (CL) in the repeated ovarian stimulated group (81.8%) had a significantly higher proliferation rate than the other groups. The proliferation rate of the OSE in the single ovarian stimulated group (56.4%) was significantly higher than that in the control group (37.5%) ( <0.01). In conclusion, single ovarian stimulation may produce more oocytes/embryos. However, repeated gonadotropin stimulation may have a negative effect on the ovarian follicular quality, the number of mature retrieved oocytes, and the embryo quality, even increasing the chance of ovarian cancer.

关键词： gonadotropin-releasing hormone     ovarian reserve     embryo developmental ability     ovarian surface epithelium    

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Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 185-190 doi: 10.15302/J-FASE-2014035

摘要： DNA damage is one of the most common threats to meiotic cells. It has the potential to induce infertility and genetic abnormalities that may be passed to the embryo. Here, we reviewed exogenous factors which could induce DNA damage. Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic development. Complex DNA damage, double-strand breaks, represents a more difficult repair process and involves various repair pathways. Understanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for ovarian cancer and fertility preservation.

关键词： DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

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Cryopreservation of farm animal gametes and embryos: recent updates and progress

Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU

《农业科学与工程前沿（英文）》 2019年 第6卷 第1期   页码 42-53 doi: 10.15302/J-FASE-2018231

摘要：

Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously refined over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been significant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.

关键词： vitrification     gametes     embryo     animal production     cryoprotective agent     freezability    

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Comparison of prechilling stratification and sulfuric acid scarification on seed germination of

Nan WANG, Jing GAO, Suiqi ZHANG, Feng YAN

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 220-227 doi: 10.15302/J-FASE-2017146

摘要： In semi-arid regions of the Loess Plateau, water deficiency restricts plant performance. (switchgrass), which is a highly versatile grass, had been introduced to the Plateau as a restoration species. To determine if prechilling stratification (PCS) and sulfuric acid scarification (SAS) can optimize establishment, cvs Pathfinder, Trailblazer and Alamo were tested under different ambient water potentials by measuring germination and root and shoot growth along water potential gradients under laboratory conditions. Both PCS and SAS improved total germination percentage (TGP), with PCS being more beneficial. The effect of PCS and SAS on mean germination time (MGT) weakened gradually with increasing drought stress. Both PCS and SAS showed no obvious effect on promoting root and shoot growth. Both PCS and SAS reduced base water potential requirement for reaching 50% germination of Pathfinder and Trailblazer, with this effect greater for PCS. These results indicate that embryo dormancy may be a major factor limiting germination of under drought conditions. Pathfinder appears to be more suitable for a semi-arid environment, whereas Alamo appears to be unsuitable for drought conditions. Given the large difference between predicted value and measured value, the reliability and applicable scope of linear regression estimated Y needs further investigation, specification and optimization.

关键词： base water potential     data analysis method     embryo growth     germination    

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病毒性疾病对奶牛繁殖力的重要影响 Review

D. Claire Wathes, Chike F. Oguejiofor, Carole Thomas, Zhangrui Cheng

《工程（英文）》 2020年 第6卷 第1期   页码 26-33 doi: 10.1016/j.eng.2019.07.020

摘要：

世界各地的牛群中有许多病毒性疾病是地方性疾病。许多病毒穿过胎盘并导致流产和胎儿畸形的能力是众所周知的。还有大量证据表明，病毒感染对于奶牛还有其他影响，反映在受胎率的降低上。但是，这些影响很大程度上取决于单个动物首次感染该疾病的时间，因此难以量化。本文介绍了5种可能影响奶牛繁殖力的病毒，以及它们的潜在作用机制。妊娠中期非细胞病变型牛病毒性腹泻病毒（bovine viral diarrhea virus, BVDV）的急性感染会使流产率升高或导致持续感染的犊牛出生。在临近配种期感染BVDV会直接影响卵巢和子宫内膜，导致发情周期不规律和早期胚胎死亡。BVDV诱发的免疫抑制也可能降低繁殖力，从而增加对细菌的易感性。牛疱疹病毒（bovine herpesvirus, BHV）-1型在青春期前的小母牛中最常见，会导致它们生长减缓，延迟繁殖并提高首次产犊的年龄，先前受感染的动物继而表现出繁殖力的降低。尽管这可能与肺损伤有关，但也有卵巢病变的相关报告。初次感染后，BHV-1和BHV-4都潜伏在宿主中，并且可能在以后由于应激而重新激活，如与产犊和早期泌乳有关的应激。虽然仅感染BHV-4可能不会降低繁殖力，但它似乎与已建立的细菌病原体（如大肠杆菌和化脓隐秘杆菌）共同作用，促进子宫内膜炎的发展并延迟产犊后母牛的子宫修复机制。施马伦贝格病毒（Schmallenberg virus, SBV）和蓝舌病病毒（bluetongue virus, BTV）均以昆虫作为媒介传播，导致流产率和先天畸形的增加。BTV-8同时还损害孵出囊泡的发育；此外，任何一种病毒在繁殖前后的感染基本都会降低受胎率。尽管受胎率的降低通常难以量化，但足以造成经济损失，这有助于衡量疫苗接种和根除方案的效益。

关键词： 牛病毒性腹泻病毒     牛疱疹病毒1型     牛疱疹病毒4型     施马伦贝格病毒     蓝舌病毒     免疫抑制     胚胎死亡率    

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标题 作者 时间 类型 操作

Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo

null

期刊论文

Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo

null

期刊论文

Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

期刊论文

加速胚胎移植技术产业化促进我国养牛业发展

郭志勤

期刊论文

Factors affecting early embryonic development in cattle: relevance for bovine cloning

Yanna DANG, Kun ZHANG

期刊论文

Reproductive toxicity study with a novel deoxyguanosine analogue (Metacavir) in pregnant SD rats

null

期刊论文

Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface epithelium in mice

Linlin LIANG, Bei XU, Guijin ZHU

期刊论文

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

期刊论文

Cryopreservation of farm animal gametes and embryos: recent updates and progress

Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU

期刊论文

Comparison of prechilling stratification and sulfuric acid scarification on seed germination of

Nan WANG, Jing GAO, Suiqi ZHANG, Feng YAN

期刊论文

病毒性疾病对奶牛繁殖力的重要影响

D. Claire Wathes, Chike F. Oguejiofor, Carole Thomas, Zhangrui Cheng

期刊论文