[ 1 ] Star B, Nederbragt A J, Jentoft S, et al. The genome sequence of Atlantic cod reveals a unique immune system [J]. Nature. 2011; 477(7363): 207–210. link1

[ 2 ] Jones F C, Grabherr M G, Chan Y F, et al. The genomic basis of adaptive evolution in threespine sticklebacks [J]. Nature. 2012; 484(7392): 55–61. link1

[ 3 ] Nakamura Y, Morib K, Saitoh K, et al. Evolutionary changes of multiple visual pigment genes in the complete genome of pacific bluefin tuna [J]. PNAS USA. 2013; 110(27): 11061–11066. link1

[ 4 ] Smith J J, Kuraku S, Holt C, et al. Sequencing of the Sea Lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution [J]. Nat Genet. 2013; 45(4): 415–421. link1

[ 5 ] Amemiya C T, Alfoldi J, Lee A P, et al. The African Coelacanth genome provides insights into tetrapod evolution [J]. Nature. 2013; 496(7445): 311–316. link1

[ 6 ] Berthelot C, Brunet F, Chalopin D, et al. The Rainbow Trout genome provides novel insights into evolution after whole-genome duplication in vertebrates [J]. Nat commun. 2014; 5: 3657. link1

[ 7 ] David B, Catherine E W, Yang I L, et al. The genomic substrate for adaptive radiation in African Cichlid Fish [J]. Nature. 2014; 513(7518): 375–381. link1

[ 8 ] Zhang G, Fang X, Guo X, et al. The Oyster genome reveals stress adaptation and complexity of shell formation [J]. Nature. 2012; 490(7418): 49–54. link1

[ 9 ] Chen S, Zhang G, Shao C, et al. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle [J]. Nat Genet. 2014; 46(3): 253–260. link1

[10] Xu P, Zhang X, Wang X, et al. Genome sequence and genetic diversity of the common carp (Cyprinus carpio) [J]. Nat Genet. 2014; 46(11): 1212–1219. link1

[11] Wu C, Zhang D, Kan M, et al. The draft genome of the Large Yellow Croaker reveals well-developed innate immunity [J]. Nat Commun. 2014; 5: 5227. link1

[12] Wang Y, Lu Y, Zhang Y, et al. The draft genome of the Grass Carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation [J]. Nat Genet. 2015; 47(8): 625–631. link1

[13] You X X, Bian C, Zan Q J, et al. Mudskipper genomes provide insights into the terrestrial adaptation of amphibious fishes [J]. Nat Commun. 2014; 5: 5594. link1

[14] Gao Y, Gao Q, Zhang H, et al. Draft sequencing and analysis of the genome of pufferfish takifugu flavidus [J]. DNA Res. 2014; 21(6): 627–637. link1

[15] Ye N, Zhang X, Miao M, et al. Saccharina genomes provide novel insight into kelp biology [J]. Nat Commun. 2015; 6: 6986. link1

[16] Matsuda M, Nagahama Y, Shinomiya A, et al. DMY is a Y-specific DM-domain gene required for male development in the medaka fish [J]. Nature. 2002; 417(6888): 559–563. link1

[17] Hattori R S, Murai Y, Oura M, et al. A Y-linked anti-mullerian hormone duplication takes over a critical role in sex determinat ion [J]. PNAS USA. 2012; 109(8): 2955–2959. link1

[18] Myosho T, Otake H, Masuyama H, et al. Tracing the emergence of a novel sex determining gene in medaka, Oryzias luzonensis [J]. Genetics. 2012; 191(1): 163–170. link1

[19] Kamiya T, Kai W, Tasumi S, et al. A trans-species missense SNP in Amhr2 is associated with sex determination in the tiger pufferfish, takifugu rubripes (Fugu) [J]. PLoS Genet. 2012; 8(7): e1002798. link1

[20] Yano A, Guyomard R, Nicol B, et al. An immune-related gene evolved into the master sex-determining gene in rainbow trout, Oncorhynchus mykiss [J]. Curr Biol. 2012; 22(15): 1423–1428. link1

[21] Meng L, Zhu Y, Zhang N, et al. Cloning and characterization of tesk1, a novel spermatogenesis-related gene, in half-smooth tongue sole (Cynoglossus semilaevis) [J]. PLoS One. 2014; 9(10): e107922. link1

[22] Hu Q, Zhu Y. Liu Y, et al. Cloning and characterization of wnt4a gene and evidence for positive selection in half-smooth tongue sole (Cynoglossus semilaevis) [J]. Sci Rep. 2014; 4: 7167. link1

[23] Shao C, Liu G, Liu S, et al. Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts [J]. Acta Oceanol Sin. 2014; 32(8): 35–43. link1

[24] Li M H, Sun Y L, Zhao J Y, et al. A tandem duplicate of anti-müllerian hormone with a missense SNP on the Y chromosome is essential for male sex determination in nile tilapia, Oreochromis niloticus [J]. PLoS Genet. 2015; 11(11): e1005678. link1

[25] Liu Y, Zhang Y B, Liu T K, et al. Lineage-specific expansion 055中国工程科学 2016 年 第 18 卷 第 3 期of IFIT gene family: an insight into coevolution with IFN gene family [J]. PloS One. 2013; 8: e66859.

[26] Sun F, Zhang Y B, Jiang J, et al. Gig1, a novel antiviral effector involved in fish interferon response [J]. Virology. 2014; 448: 322–332. link1

[27] Wang B, Zhang Y B, Liu T K, et al. Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway [J]. Dev Comp Immunol. 2014; 47(1): 140–149. link1

[28] Zhang J, Zhang Y B, Wu M, et al. Fish MAVS is involved in RLR pathway-mediated IFN response [J]. Fish Shellfish Immunol. 2014; 41(2): 222–230. link1

[29] Wang N, Wang X, Yang C, et al. Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot, Scophthalmus maximus [J]. Fish Shellfish Immunol. 2014; 35(4): 1200–1208. link1

[30] Wang N, Wang X, Yang C, et al. Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus) [J]. Dev Comp Immunol. 2014; 43(1): 96–105. link1

[31] Chen S, Li W, Meng L, et al. Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus) [J]. Fish Shellfish Immunol. 2014; 22(3): 172–181. link1

[32] Yang C, Wang X, Zhang B, et al. Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) [J]. BMC Mol Biol. 2013; 14: 10. link1

[33] Zeng Y, Xiang J, Lu Y, et al. SghC1q, a novel C1q family member from half-smooth tongue sole (Cynoglossus semilaevis): identification, expression and analysis of antibacterial and antiviral activities [J]. Dev Comp Immunol. 2015; 48(1): 151–163. link1

[34] Lu Y, Wang Q, Liu Y, et al. Gene cloning and expression analysis of IRF1 in half-smooth tongue sole (Cynoglossus semilaevis) [J]. Mol Biol Rep. 2014; 41(6): 4093–4101. link1

[35] Lien S, Gidskehaug L, Moen T, et al. A dense SNP-based linkage map for Atlantic Salmon (Salmo salar) reveals extended chromosome homeologies and striking differences in sex-specific recombination patterns [J]. BMC Genomics. 2011; 12: 615. link1

[36] Guyomard R, Boussaha M, Krieg F, et al. A synthetic Rainbow Trout linkage map provides new insights into the salmonid whole genome duplication and the conservation of synteny among teleosts [J]. BMC Genetics. 2012; 13: 15. link1

[37] Carlson B M, Onusko S W, Gross J B. A high-density linkage map for astyanax mexicanus using genotyping-by-sequencing technology [J]. Genes, Genomes, Genetics (Bethesda). 2014; 5(2): 241–251. link1

[38] Li Y, Liu S, Qin Z, et al. Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish [J]. DNA Res. 2015; 22(1): 39–52. link1

[39] Shao C, Niu Y, Pasi R, et al. Genome-wide snp identification for the construction of a high-resolution genetic map of Japanese flounder (Paralichthys olivaceus) applied to QTL mapping of vibrio anguillarum disease resistance and comparative genomic analysis [J]. DNA Res. 2015; 22(2): 161–170. link1

[40] Wang W, Hu Y, Ma Y, et al. High-density genetic linkage mapping in turbot (Scophthalmus maximus) based on SNP markers and major sex-and growth-related regions detection [J]. PLoS One. 2015; 10(3): e0120410. link1

[41] Zhang X, Zhang Y, Zheng X, et al. A consensus linkage map provides insights on genome character and evolution in common carp (Cyprinus carpio) [J]. Mar Biotechnol. 2013; 15(3): 275–312. link1

[42] Li H, Liu X, Zhang G. A consensus microsatellite-based linkage map for the hermaphroditic bay scallop (Argopecten irradians) and its application in size-related QTL analysis [J]. PLoS One. 2012; 7(10): e46926. link1

[43] Yu Y, Zhang X J, Yuan J B, et al. Genome survey and high-density genetic map construction provide genomic and genetic resources for the pacific white shrimp litopenaeus vannamei [J]. Sci Rep. 2015; 5: 15612. link1

[44] Jiao W, Fu X, Dou J, et al. High-resolution linkage and quantitative trait locus mapping aided by genome survey sequencing: building up an integrative genomic framework for a bivalve mollusc [J]. DNA Res. 2014; 21(1): 85–101. link1

[45] Zhang N, Zhang L, Tao Y, et al. Construction of a high density SNP linkage map of kelp (Saccharina japonica) by sequencing Taq I site associated DNA and mapping of a sex determining locus [J]. BMC Genomics. 2015; 16(1): 189. link1

[46] Ninwichia P, Peatman E, Perera D, et al. Identification of a sex-linked marker for channel catfish [J]. Anim Genet. 2011; 43(4): 476–477. link1

[47] Lipinska A P, Ahmed S, Peters A F, et al. Development of PCR-based markers to determine the sex of kelps [J]. PLoS One. 2015; 10(10): e0140535. link1

[48] Campbell N R, LaPatra S E, Overturf K, et al. Association mapping of disease resistance traits in rainbow rout using restriction site associated DNA sequencing [J]. Genes, Genomes, Genetics (Bethesda). 2014; 4(12): 2473–2481. link1

[49] Rodriguez-Ramilo S T, Fernandez J, Toro M A, et al. Uncovering QTL for resistance and survival time to philasterides dicentrarchi in turbot (Scophthalmus maximus) [J]. Anim Genet. 2013; 44(2): 149–157. link1

[50] Dutta S, Biswas S, Mukherjee K, et al. Identification of RAPD-SCAR marker linked to white spot syndrome virus resistance in populations of giant black tiger shrimp, penaeus monodon fabricius [J]. J Fish Dis. 2014; 37(5): 471–480. link1

[51] 陈松林. 鱼类性别控制与细胞工程育种[M]. 北京: 科学出版社, 2013.

[52] Chen S L, Li J, Deng S P, et al. Isolation of female specific AFLP markers and identification of genetic sex in half-smooth tongue sole (Cynoglossus semilaevis) [J]. Mar Biotechnd (NY) 2007; 9(2): 272–280. link1

[53] Chen S L, Ji X S, Shao C W, et al. Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole (Cynoglossus semilaevis) [J]. Mar Biotechnol. 2012; 14(1): 120–128. link1

[54] Dan C, Mei J, Wang D, et al. Genetic differentiation and efficient sex-specific marker development of a pair of Y- and X-linked markers in yellow catfish [J]. Int J Biol Sci. 2013; 9(10): 1043–1049. link1

[55] Xu D D, Lou B, Xu H X, et al. Isolation and characterization of male-specific DNA markers in the rock bream oplegnathus 056综合研究   水产生物技术发展战略研究Fasciatus [J]. Mar Biotechnol. 2013; 15(2): 221–229. link1

[56] Liu Y, Bi Y, Gu J, et al. Localization of a female-specific marker on the chromosomes of the brown seaweed saccharina japonica using fluorescence in situ hybridization [J]. PLoS One. 2012; 7(11): e48784. link1

[57] Wang L, Fan C, Liu Y, et al. A genome scan for quantitative trait loci associated with Vibrio anguillarum infection resistance in Japanese flounder (Paralichthys olivaceus) by bulked segregant analysis [J]. Mar Biotechnol. 2014; 16(5): 513–521. link1

[58] Robinson N, Hayes B. Modelling the use of gene expression profiles with selective breeding for improved disease resistance in atlantic salmon (Salmo salar) [J]. Aquaculture. 2008; 285(1): 38–46. link1

[59] Liu S, Sun L, Li Y, et al. Development of the Catfish 250K SNP array for genome-wide association studies [J]. BMC Res Notes. 2014; 7: 135. link1

[60] Tatsumi Y, Takeda M, Matsuda M, et al. TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development [J]. FEBS Lett. 2014; 588(24): 4543–4550. link1

[61] Ansai S, Kinoshita M. Targeted mutagenesis using CRISPR/Cas system in medaka [J]. Biol Open. 2014; 3(5): 362–371. link1

[62] Edvardsen R B, Leininger S, Kleppe L, et al. Targeted mutagenesis in atlantic salmon (Salmo salar) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation [J]. PLoS One. 2014; 9(9): e108622. link1

[63] Li M H, Yang H H, Li M R, et al. Antagonistic roles of dmrt1 and foxl2 in sex differentiation via estrogen production in tilapia as demonstrated by TALENs [J]. Endocrinology. 2013; 154: 4814–4825. link1

[64] Zhong Z M, Niu P F, Wang M Y, et al. Targeted disruption of sp7 and myostatin with CRISPR/Cas9 results in severe bone defects and more muscular cells in common carp [J]. Sci Rep. 2016; 6: 22953. link1

[65] 陈松林, 崔忠凯, 郑汉其, 等. 一种基于基因组编辑的海水鲆鲽鱼类种质构建方法及应用[P]. 201610162019. 5. Chen S L, Cui Z K, Zheng H Q, et al. A genome editing-based breeding method in flatfish and its application [P]. 201610162019.

[66] Swaminathan T R, Basheer V S, Gopalakrishnan A, et al. Establishment of caudal fin cell lines from tropical ornamental fishes Puntius fasciatus and Pristolepis fasciata endemic to the western ghats of India [J]. Acta Tropica. 2013; 28(3): 536– 541. link1

[67] Abdul Majeed S, Nambi K S, Taju G, et al. Development, characterization and application of a new fibroblastic-like cell line from kidney of a freshwater air breathing fish channa striatus (Bloch, 1793) [J]. Acta Tropica. 2013; 127(1): 25–32. link1

[68] Jayesh P, Jose S, Philip R, et al. A novel medium for the development of in vitro cell culture system from penaeus monodon [J]. Cytotechnology. 2013; 65(3): 307–322. link1

[69] Mercurio S, Di Benedetto C, Sugni M, et al. Primary cell cultures from sea urchin ovaries: a new experimental tool [J]. In Vitro Cell Dev Biol Anim. 2014; 50(2): 139–145. link1

[70] 陈松林, 秦启伟. 鱼类细胞培养理论与技术[M]. 北京: 科学出版社, 2011.

[71] Sun A, Wang T Z, Wang N. e t a l . Es tabl i shment and characterization of an ovarian cell line from half-smooth tongue sole (Cynoglossus semilaevis) [J]. J Fish Biol. 2015; 86(1): 46–59.

[72] Sun A, Chen S, Gao F, et al. Establishment and characterization of a gonad cell line from half-smooth tongue sole (Cynoglossus semilaevis) pseudomale [J]. Fish Physiol Biochem. 2015; 41(3): 673–683. link1

[73] Wang T Z, Sun A, Wang N. e t a l . Es tabl i shment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis) [J]. Zool Res. 2015; 36 (5): 305–310.

[74] 刘肖峰, 陈松林, 沙珍霞, 等. 云纹石斑鱼心脏细胞系的建立与鉴定[J]. 农业生物技术学报, 2015, 23(10): 1394–1400. link1

[75] Keivanloo S, Sudagar M . Feasibility studies on vitrification of persian sturgeon (Acipenser persicus) embryos [J]. J Aquac Res Dev. 2013; 4: 172. link1

[76] Tian Y, Jiang J, Song L, et al. Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos [J]. Cryobiology. 2015; 71(3): 499–506. link1