2012, Volume 14, Issue 2
Strategic Study of CAE >> 2012, Volume 14, Issue 2
A quick detection method for the early stage of disease in turbot (Scophthalmus maximus)—Development of the colloidal gold based immunochromatographic strip
1. Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
3. Qingdao General Aquaculture Co., Ltd., Qingdao 266404, China
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Abstract
In this study, an immunochromatographic strip was developed for the serological detection of antibodies against bovine serum albumin (BSA) in turbot (Scophthalmus maximus), in which the BSA was taken as antigen. In the strip, the rabbit anti-turbot immunoglobulin M, made from this research, labeled with colloidal gold was used as the detector, and the BSA and staphylococcal protein A were blotted on the nitrocellulose membrane for the test and control lines, respectively. The positive serum samples from turbot vaccinated with BSA and negative serum samples from normal turbot were collected to evaluate the characteristics of the strip, and the results were compared with the method of enzyme linked immunosorbent assay (ELISA) established in this study. The strip was shown to be of high specificity and sensitivity, and similar to ELISA. Furthermore, this dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents or equipments. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis. Based on the model of this strip developed in the study, this pattern of strip could be performed into any antibody detection in aquaculture animals; it provides a rapid and simple test method to validate the efficacy of the vaccine and to detect and monitor the fish disease in time.
Keywords
turbot (Scophthalmus maximus) ; antibody ; immunochromatographic strip ; pointofcare test ; colloidal gold ; disease
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