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Strategic Study of CAE >> 2015, Volume 17, Issue 6

The lethal dose test of Ginsenoside CK and Icraiin for sperm in vitro

Henan Province Research Institute for Population and Family Planning, Zhengzhou 450002, China

Funding project:河南省属科研院所基本科研业务费专项资金项目(2014JBKY006) Received: 2015-03-10 Available online: 2015-07-06 13:39:22.000

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Abstract

Objective:To investigate the effects of Ginsenoside CK(Gin CK),Icraiin(Icr) for sperm survive in vitro. Methods:40 sample of male semen were cultured in medium and grouped it in vitro,To adjust the semen concentration is 1.0×107/mL by culture medium of F-10(F-10),To add respect Ginsenoside(Gin1 group,500 μg/mL;Gin2 group,100 μg/mL),Icraiin(Icr1 group,500 μg/mL;Icr2 group,100 μg/mL)were added respectively. F-10 alone was the control groups. To compare with them of groups sperm tmortality. Results:The values of sperm mortality after cultured for 24 h. The motality of I2 group was 41.31±17.49,F group was 47.50±19.85,G2 group was 55.75±24.93,I3 group was 58.05±19.06,G1 group was 98.84±1.62. Ranking them from less to more motality were E2 groups,F groups,I2 groups,G2 groups,I3 groups,G1 group s(p<0.05). Conclusion:When the concentration was 0.1 mg/mL,Gin CK can increase the sperm mortality significantly and Icr was not; When the concentration was 0.5 mg/mL,it is the lethal dose for Gin CK and Icr can increase the sperm mortality but it is not.

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