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Journal Article 6

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2023 6

Keywords

Glycosylation 2

Immunoglobulin G 2

Antibody response 1

Colorectal cancer 1

Diagnostic biomarker 1

Disease pathogenesis 1

GWAS 1

Gastrointestinal cancer 1

Genetics 1

Genomics 1

Glycans 1

Glycome 1

Haff disease 1

IgG glycome 1

N-glycomics 1

N-glycosylation 1

Porous graphitized carbon liquid chromatography mass spectrometry 1

Serum 1

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The Glycome and Glycomedicine

Wei Wang, Baofeng Yang

Engineering 2023, Volume 26, Issue 7,   Pages 1-2 doi: 10.1016/j.eng.2023.05.007

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Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk between IgG sialylation and pDC functions

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 549-561 doi: 10.1007/s11684-022-0965-7

Abstract: Immunoglobulin (IgG) glycosylation affects the effector functions of IgG in a myriad of biological processes and has been closely associated with numerous autoimmune diseases, including systemic lupus erythematosus (SLE), thus underlining the pathogenic role of glycosylation aberration in autoimmunity. This study aims to explore the relationship between IgG sialylation patterns and lupus pregnancy. Relative to that in serum samples from the control cohort, IgG sialylation level was aberrantly downregulated in serum samples from the SLE cohort at four stages (from preconception to the third trimester of pregnancy) and was significantly associated with lupus activity and fetal loss during lupus pregnancy. The type I interferon signature of pregnant patients with SLE was negatively correlated with the level of IgG sialylation. The lack of sialylation dampened the ability of IgG to suppress the functions of plasmacytoid dendritic cells (pDCs). RNA-seq analysis further revealed that the expression of genes associated with the spleen tyrosine kinase (SYK) signaling pathway significantly differed between IgG- and deSia-IgG-treated pDCs. This finding was confirmed by the attenuation of the ability to phosphorylate SYK and BLNK in deSia-IgG. Finally, the coculture of pDCs isolated from pregnant patients with SLE with IgG/deSia-IgG demonstrated the sialylation-dependent anti-inflammatory function of IgG. Our findings suggested that IgG influences lupus activity through regulating pDCs function via the modulation of the SYK pathway in a sialic acid-dependent manner.

Keywords： pregnancy     IgG glycome     type I interferon     systemic lupus erythematosus    

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Twelve Years of Genome-Wide Association Studies of Human Protein N-Glycosylation Review

Anna Timoshchuk, Sodbo Sharapov, Yurii S. Aulchenko

Engineering 2023, Volume 26, Issue 7,   Pages 17-31 doi: 10.1016/j.eng.2023.03.013

Abstract: The lack of a detailed understanding of the mechanisms regulating glycome variation and linking the glycomeUntil now, such studies have been limited to an analysis of the human blood plasma N-glycome

Keywords： Glycome     Glycans     N-glycosylation     Genomics     Genetics     GWAS    

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Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Article

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Engineering 2023, Volume 26, Issue 7,   Pages 63-73 doi: 10.1016/j.eng.2022.09.012

Abstract: Profiling of the N-glycome in HD would assist in deciphering the underlying molecular mechanismN-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterized

Keywords： Haff disease     Serum     Immunoglobulin G     Glycosylation     Disease pathogenesis    

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Profound Diversity of the N-Glycome from Microdissected Regions of Colorectal Cancer, Stroma Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Engineering 2023, Volume 26, Issue 7,   Pages 32-43 doi: 10.1016/j.eng.2022.08.016

Abstract:

Aberrant glycosylation is considered to be a hallmark of colorectal cancer (CRC), as demonstrated by various studies. While the N-glycosylation of cell lines and serum has been widely examined, the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures. To overcome these obstacles, we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous, stromal, and healthy mucosa cells. N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray ionization tandem mass spectrometry, enabling the differentiation and structural characterization of isomeric species. In total, 116 N-glycans were identified that showed profound differences in expression among cancer, stroma, and normal mucosa. In comparison with healthy mucosa, the cancer cells showed an increase in α2-6 sialylation and monoantennary N-glycans, as well as a decrease in bisected N-glycans. Moreover, specific sialylated and (sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers. In comparison with cancer, the stroma showed lower levels of oligomannosidic and monoantennary N-glycans, LewisA/X epitopes, and sulfation, as well as increased expression of (core-)fucosylation and α2-3 sialylation. Our study reveals the distinct N-glycomic profiles of different cell types in CRC tumor and control tissues, proving the necessity of their separate analysis for the discovery of cancer-associated glycans.

 

Keywords： Colorectal cancer     Tumor     Porous graphitized carbon liquid chromatography mass spectrometry     N-glycomics     Antibody response    

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High-Throughput Profiling of Serological Immunoglobulin G N-Glycome as a Noninvasive Biomarker Article

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Engineering 2023, Volume 26, Issue 7,   Pages 44-53 doi: 10.1016/j.eng.2023.02.008

Abstract: liquid chromatography (HILIC-UPLC) was employed to analyze the composition of the plasma IgG N-glycome

Keywords： Gastrointestinal cancer     Glycosylation     Immunoglobulin G     Diagnostic biomarker    

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Title Author Date Type Operation

The Glycome and Glycomedicine

Wei Wang, Baofeng Yang

Journal Article

Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk between IgG sialylation and pDC functions

Journal Article

Twelve Years of Genome-Wide Association Studies of Human Protein N-Glycosylation

Anna Timoshchuk, Sodbo Sharapov, Yurii S. Aulchenko

Journal Article

Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Journal Article

Profound Diversity of the N-Glycome from Microdissected Regions of Colorectal Cancer, Stroma

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Journal Article

High-Throughput Profiling of Serological Immunoglobulin G N-Glycome as a Noninvasive Biomarker

Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou

Journal Article