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Turbot (Scophthalmus maximus L.) Neuropeptide Y Starvation Feeding Differential expression 1

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The Present Status and Sustainable Development of Turbot (Scophthalmus maximus L.) Culture in China

Lei Jilin,Ma Aijun,Chen Chao,Zhuang Zhimeng

Strategic Study of CAE 2005, Volume 7, Issue 5,   Pages 30-34

Abstract:

With the development of turbot cultivation and market, turbot has been proved to be a high-quality species grown-out in China. After several-year practice, the primary intensive culture model has formed in the northern part of China. The production of turbot fries in the year 2004 is around 35 million and the commercial fish 20 thousand tonnes. As a result, China has become one of the largest countries in turbot culture and consumption in the world. Turbot culture industry has not only made great contribution to the development of marine culture industry and the littoral areas economy but also laid a theoretical and practical foundation for the intensive marine fish culture industry in China. Particularly, the accession of China to WTO brought new development opportunity for turbot culture. Those new ideas and objectives are driving the turbot culture industry into an updated stage, on which turbot will be developed in a standardized, large scale genetic breeding, the industry of special formula feeds with balanced nutrition, and the recirculation system.

Keywords: turbot (Scophthalmus maximus)     present status of culture     sustainable development    

Optimization technology of spices-smoked Scophthalmus maximus

Wang Caili,Yuan Deshun,Sun Aihua,Li Hui,Teng Yu

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 61-63

Abstract:

Scophthalmus maximus was a kind of dietary supplement and idealThe cold-smoked processing technology of Scophthalmus maximus was studied, and the results showed that

Keywords: Cold-smoke     Scophthalmus maximus     spices     pickle    

cDNA Cloning of Neuropeptide Y gene of turbot Scophthalmus maximus L. and its expression analysis of

Han Jian,Liu Bin,Jiang Zhiqiang,Mu Xiaosheng,Lei Jilin

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 42-49

Abstract:

In this study, the full length of turbot NPY cDNA was cloned using RT-PCR and rapid-amplification of cDNA ends (RACE). Turbot NPY cDNA is 729bp with a 5´-untranslated region (UTR), 135bp 3´-UTR and 299bp ORF. The open reading frame encodes a 99 amino acid NPY. The first 28 amino acids constitute the signal sequence followed by the 36 amino acid mature peptide. Turbot NPY share a 53.9–98.0% identity with NPY from other fish, especially, Turbot NPY share a 97–98.0% identity with NPY from other flounder (Japanese flounder, winter flounder).Using real-time PCR to analysis NPY expression in turbot. Tissue distribution studies show that NPY mRNA is expressed in brain, in particular the hypothalamic, and in peripheral tissues. During food deprivation, the NPY undergo significant change in expression (P<0.05), with NPY levels being elevated first, then decreased, and being significantly increased, maintain a high level of expression, then decreased again during food deprivation. Expression level was significantly higher with refeeding after fasting than the normal feeding, the longer of food deprivation, the higher of expression level. But the expression level of 2h after refeeding will fall again to a similar level. Our results suggest that NPY is conserved peptides that might be involved in the regulation of feeding and other physiological functions in turbot.

Keywords: Turbot (Scophthalmus maximus L.) Neuropeptide Y Starvation Feeding Differential expression    

Development and characterization of monoclonal antibody to the immunoglobulin of turbot (Scophthalmus maximus

Wang Weifang,Li Qingmei,Chai Shujun,Gan Lingling,Hong Lei,Guo Junqing,Lei Jilin

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 16-20

Abstract:

Four monoclonal antibodies (mAbs) to immunoglobulin M (IgM) of turbot Scophthalmus maximusmaximus, light specificity for Paralichthys olivaceus

Keywords: turbot (Scophthalmus     maximus)     immunoglobulin M (IgM)     monoclonal antibody     BALB/C mice    

A quick detection method for the early stage of disease in turbot (Scophthalmus maximus)—Development

Wang Weifang,Chai Shujun,Liu Qingtang,Lei Jilin,Ding Fuhong,Hong Lei,Liu Xinfu,Su Ke

Strategic Study of CAE 2012, Volume 14, Issue 2,   Pages 8-13

Abstract: the serological detection of antibodies against bovine serum albumin (BSA) in turbot (Scophthalmus maximus

Keywords: turbot (Scophthalmus maximus)     antibody     immunochromatographic strip     pointofcare test     colloidal gold    

Title Author Date Type Operation

The Present Status and Sustainable Development of Turbot (Scophthalmus maximus L.) Culture in China

Lei Jilin,Ma Aijun,Chen Chao,Zhuang Zhimeng

Journal Article

Optimization technology of spices-smoked Scophthalmus maximus

Wang Caili,Yuan Deshun,Sun Aihua,Li Hui,Teng Yu

Journal Article

cDNA Cloning of Neuropeptide Y gene of turbot Scophthalmus maximus L. and its expression analysis of

Han Jian,Liu Bin,Jiang Zhiqiang,Mu Xiaosheng,Lei Jilin

Journal Article

Development and characterization of monoclonal antibody to the immunoglobulin of turbot (Scophthalmus maximus

Wang Weifang,Li Qingmei,Chai Shujun,Gan Lingling,Hong Lei,Guo Junqing,Lei Jilin

Journal Article

A quick detection method for the early stage of disease in turbot (Scophthalmus maximus)—Development

Wang Weifang,Chai Shujun,Liu Qingtang,Lei Jilin,Ding Fuhong,Hong Lei,Liu Xinfu,Su Ke

Journal Article