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Properties and catalytic mechanism of -glutamyltranspeptidase from NX-2

WANG Qian, YAO Zhong, XUN Zhijing, XU Xiaoying, XU Hong, WEI Ping

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 4,   Pages 456-461 doi: 10.1007/s11705-008-0075-3

Abstract: At the conditions of pH 10.0, D-glutamine (D-Gln)/L-tryptophan (L-Trp) with a molar ratio of 5 : 7, a(-D-Gln-L-Trp) was discussed.The affinity parameter of GGT to D-Gln (Km) was 5.08 mmol·L and the maximum reaction rate of transpeptidation() was determined as 0.034 mmol·min·L, while the affinity parameter of GGT to -D-Gln-L-Trp (’) was 2.267mmol·L, and the maximum reaction rate of hydrolysis (’) was 0.012 mmol·min·L.

Keywords： catalytic mechanism     mmol·min·L     D-glutamine     affinity parameter     important practical    

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Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine

CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 220-223 doi: 10.1007/s11705-008-0041-0

Abstract: L-4-Fluorophenylalanine (FPhe) was prepared from 4-fluorophenylpyruvate (FPPA) catalyzed by aspartateconditions: 37°C, pH value range of 5.0–8.0, 5.5 mass ratio of cell to FPPA, 0.6% (w/v) of Tween 80, 7.08 g/LFPPA, and 1.6 of molar ratio of L-Asp to FPPA.

Keywords： L-4-Fluorophenylalanine     Asp-AT     aspartate aminotransferase     4-fluorophenylpyruvate     FPPA    

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Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 89-94 doi: 10.1007/s11783-007-0017-1

Abstract: glucose feeding on the ligninolytic enzyme production of in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium.The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculationCompared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (finalcontrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L.

Keywords： enzyme production     fed-batch culture     nitrogen-limited     inoculation     mmol/L    

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Microbial communities biostimulated by ethanol during uranium (VI) bioremediation in contaminated sediment as shown by stable isotope probing

Mary Beth LEIGH,Wei-Min WU,Erick CARDENAS,Ondrej UHLIK,Sue CARROLL,Terry GENTRY,Terence L.

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 3,   Pages 453-464 doi: 10.1007/s11783-014-0721-6

Abstract: Stable isotope probing (SIP) was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sample was highly bioreduced with ethanol while another was less reduced. Microcosms with the respective sediments were amended with C labeled ethanol and incubated for 7 days for SIP. Ethanol was rapidly converted to acetate within 24 h accompanied with the reduction of nitrate and sulfate. The accumulation of acetate persisted beyond the 7 d period. Aqueous U did not decline in the microcosm with the reduced sediment due to desorption of U but continuously declined in the less reduced sample. Microbial growth and concomitant C-DNA production was detected when ethanol was exhausted and abundant acetate had accumulated in both microcosms. This coincided with U(VI) reduction in the less reduced sample. C originating from ethanol was ultimately utilized for growth, either directly or indirectly, by the dominant microbial community members within 7 days of incubation. The microbial community was comprised predominantly of known denitrifiers, sulfate-reducing bacteria and iron (III) reducing bacteria including , , , and others, including the known U(VI)-reducing bacteria A , and The findings suggest that ethanol biostimulates the U(VI)-reducing microbial community by first serving as an electron donor for nitrate, sulfate, iron (III) and U(VI) reduction, and acetate which then functions as electron donor for U(VI) reduction and carbon source for microbial growth.

Keywords： Stable isotope probing (SIP)     ethanol     acetate     uranium reduction     sediment     bioremediation    

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Comments on the special issue on forestry of

John L. INNES

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 4,   Pages 502-503 doi: 10.15302/J-FASE-2017189

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PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives

Yumeng Wang, Guiling Li

Frontiers of Medicine 2019, Volume 13, Issue 4,   Pages 438-450 doi: 10.1007/s11684-018-0674-4

Abstract: Checkpoint blockades targeting the PD-1/PD-L1 axis have achieved effective clinical responses with acceptableIn this review, we summarize recent advances in our understanding of the PD-1/PD-L1 signaling pathway, including the expression patterns of PD-1/PD-L1 and potential PD-1/PD-L1-related therapeutic strategies

Keywords： PD-1     PD-L1     immune checkpoint blockade antibody     immunotherapy     cervical cancer    

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In memory of Perry L. McCarty, seminal founder of Environmental Biotechnology

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 10, doi: 10.1007/s11783-023-1730-0

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Shear design of high strength concrete prestressed girders

Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4,   Pages 373-387 doi: 10.1007/s11709-014-0087-7

Abstract: Normal strength prestressed concrete I-girders are commonly used as the primary superstructure components in highway bridges. However, shear design guidelines for high strength PC girders are not available in the current structural codes. Recently, ten 7.62 m (25 feet) long girders made with high strength concrete were designed, cast, and tested at the University of Houston (UH) to study the ultimate shear strength and the shear concrete contribution ( ) as a function of concrete strength ( ). A simple semi-empirical set of equations was developed based on the test results to predict the ultimate shear strength of prestressed concrete I-girders. The UH-developed set of equations is a function of concrete strength ( ), web area ( ), shear span to effective depth ratio ( / ), and percentage of transverse steel ( ). The proposed UH-Method was found to accurately predict the ultimate shear strength of PC girders with concrete strength up to 117 MPa (17000 psi) ensuring satisfactory ductility. The UH-Method was found to be not as overly conservative as the ACI-318 (2011) code provisions, and also not to overestimate the ultimate shear strength of high strength PC girders as the AASHTO LRFD (2010) code provisions. Moreover, the proposed UH-Method was found fairly accurate and not exceedingly conservative in predicting the concrete contribution to shear for concrete strength up to 117 MPa (17000 psi).

Keywords： shear design     high strength concrete     prestressed girders     full-scale tests    

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Introduction to the special section on the 12

Zhen-Min CHENG

Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 1,   Pages 1-1 doi: 10.1007/s11705-009-0003-1

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Deeply missing my career idol and role model ― Professor Perry L. McCarty

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 9, doi: 10.1007/s11783-023-1717-x

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Distributed coordination inmulti-agent systems: a graph Laplacian perspective

Zhi-min HAN,Zhi-yun LIN,Min-yue FU,Zhi-yong CHEN

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 6,   Pages 429-448 doi: 10.1631/FITEE.1500118

Abstract: This paper reviews some main results and progress in distributed multi-agent coordination from a graph Laplacian perspective. Distributed multi-agent coordination has been a very active subject studied extensively by the systems and control community in last decades, including distributed consensus, formation control, sensor localization, distributed optimization, etc. The aim of this paper is to provide both a comprehensive survey of existing literature in distributed multi-agent coordination and a new perspective in terms of graph Laplacian to categorize the fundamental mechanisms for distributed coordination. For different types of graph Laplacians, we summarize their inherent coordination features and specific research issues. This paper also highlights several promising research directions along with some open problems that are deemed important for future study.

Keywords： Multi-agent systems     Distributed coordination     Graph Laplacian    

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Urban watershed management under rapid urbanization

Pengfei DU, Haifeng JIA, Shaw L. YU

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 5,   Pages 595-595 doi: 10.1007/s11783-012-0452-5

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Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L.

Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo

Frontiers of Medicine 2017, Volume 11, Issue 1,   Pages 137-146 doi: 10.1007/s11684-016-0486-3

Abstract: The effects of different Cd concentrations (0, 20, 60, and 120 μmol/L) on the biosynthesis of Artemisia annua L. were examined.The results showed that the application of treatment with 60 and 120 μmol/L Cd for 3 days significantlyThe concentrations of artemisinic acid, arteannuin B, and artemisinin in the 120 μmol/L Cd-treated;DBR2, ALDH1, and DXR were up-regulated in the 120 μmol/L

Keywords： Cd     secondary metabolites     gene expressions     Artemisia annua L.    

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Reactive extraction for preparation of hydrogen peroxide under pressure

CHENG Yongxi, LI Hongtao, WANG Li, LÜ Shuxiang

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 3,   Pages 335-340 doi: 10.1007/s11705-008-0042-z

Abstract: The preparation of hydrogen peroxide from anthrahydroquinone by reactive extraction was investigated. The integration process of oxidation of anthrahydroquinone by air and extraction of hydrogen peroxide from the organic phase with water was carried out in a sieve plate column under pressure. The conversion of anthrahydroquinone increased with increasing pressure resulting in an increase of hydrogen peroxide concentration in the aqueous phase. However, no change in extraction efficiency of hydrogen peroxide was observed. A mathematical model for gas-liquid-liquid reactive extraction was established. In the model, the effects of pressure and gas superficial velocity on reaction were considered. With increasing gas superficial velocity, the conversion of anthrahydroquinone increased, and the fraction of hydrogen peroxide extracted reached a plateau with a maximum of 72.94%. However, both the conversion of anthrahydroquinone and the fraction of hydrogen peroxide extracted decreased with increasing organic phase superficial velocity.
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Advances in Underground Construction Help Provide Quality of Life for Modern Societies

Raymond L. Sterling

Engineering 2017, Volume 3, Issue 6,   Pages 780-781 doi: 10.1016/j.eng.2017.12.006

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Title Author Date Type Operation

Properties and catalytic mechanism of -glutamyltranspeptidase from NX-2

WANG Qian, YAO Zhong, XUN Zhijing, XU Xiaoying, XU Hong, WEI Ping

Journal Article

Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine

CHEN Meijuan, JIA Honghua, CHEN Yongsheng, JIANG Min, WEI Ping

Journal Article

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Journal Article

Microbial communities biostimulated by ethanol during uranium (VI) bioremediation in contaminated sediment as shown by stable isotope probing

Mary Beth LEIGH,Wei-Min WU,Erick CARDENAS,Ondrej UHLIK,Sue CARROLL,Terry GENTRY,Terence L.

Journal Article

Comments on the special issue on forestry of

John L. INNES

Journal Article

PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives

Yumeng Wang, Guiling Li

Journal Article

In memory of Perry L. McCarty, seminal founder of Environmental Biotechnology

Journal Article

Shear design of high strength concrete prestressed girders

Emad L. LABIB,Hemant B. DHONDE,Thomas T. C. HSU,Y. L. MO

Journal Article

Introduction to the special section on the 12

Zhen-Min CHENG

Journal Article

Deeply missing my career idol and role model ― Professor Perry L. McCarty

Journal Article

Distributed coordination inmulti-agent systems: a graph Laplacian perspective

Zhi-min HAN,Zhi-yun LIN,Min-yue FU,Zhi-yong CHEN

Journal Article

Urban watershed management under rapid urbanization

Pengfei DU, Haifeng JIA, Shaw L. YU

Journal Article

Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L.

Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo

Journal Article

Reactive extraction for preparation of hydrogen peroxide under pressure

CHENG Yongxi, LI Hongtao, WANG Li, LÜ Shuxiang

Journal Article

Advances in Underground Construction Help Provide Quality of Life for Modern Societies

Raymond L. Sterling

Journal Article