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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Frontiers of Medicine 2022, Volume 16, Issue 3,   Pages 378-388 doi: 10.1007/s11684-021-0840-y

Abstract: The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of

Keywords： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein    

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Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-

Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG

Frontiers of Chemical Science and Engineering 2014, Volume 8, Issue 4,   Pages 433-444 doi: 10.1007/s11705-014-1454-6

Abstract: The aggregation of amyloid -protein (A ) is tightly linked to the pathogenesis of Alzheimer’s diseaseHowever, atomic details of binding modes and binding affinities between these peptide inhibitors andAll inhibitors exhibit varied binding affinity to A , in which KLVFF has the highest binding affinityThis work provides structure-based binding information for further modification and optimization of thesethree peptide inhibitors to enhance their binding and inhibitory abilities against A aggregation.

Keywords： Alzheimer’s disease     amyloid β-protein     peptide inhibitors     protein-protein interaction     molecular    

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Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 26-29 doi: 10.1007/s11684-009-0008-7

Abstract: clone formation assay, and western blot was applied to detect the expressions of Ku80, DNA-PKcs and ATM proteinThe correlativity of protein expression with SF2 value was analyzed by Pearson linear correlation analysisWe found that the expression of same protein in different cell lines and the expression of three proteinsThese findings suggest that the expression level of DNA-PKcs protein can be an indicator for predicting

Keywords： Ku80     DNA-PK(cs)-binding protein     human     ataxia telangiectasia mutated protein     tumor cell lines     radiosensitivity    

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Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk betweenIgG sialylation and pDC functions

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 549-561 doi: 10.1007/s11684-022-0965-7

Abstract: Immunoglobulin (IgG) glycosylation affects the effector functions of IgG in a myriad of biological processesThis study aims to explore the relationship between IgG sialylation patterns and lupus pregnancy.- and deSia-IgG-treated pDCs.Finally, the coculture of pDCs isolated from pregnant patients with SLE with IgG/deSia-IgG demonstratedthe sialylation-dependent anti-inflammatory function of IgG.

Keywords： pregnancy     IgG glycome     type I interferon     systemic lupus erythematosus    

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Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose

Dongmei WANG, Guodong JIA, Liang XU, Xiaoyan DONG, Yan SUN

Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 3,   Pages 229-234 doi: 10.1007/s11705-009-0213-6

Abstract: The dynamic binding capacity (DBC) in the presence of 2D eEF was higher than in the presence of a one-dimensional2D electrochromatography packed with the superporous cellulose adsorbent is promising for high-speed protein

Keywords： electrochromatography     two-dimensional electric field     dynamic binding capacity     superporous cellulose bead     protein    

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Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 2,   Pages 97-104 doi: 10.15302/J-FASE-2018248

Abstract:

Plant nucleotide binding domain and leucine-rich repeat (NLR) receptors recognize pathogen effectorsThe N-end rule pathway of protein degradation is also directly linked to NLR stability.

Keywords： E3 ubiquitin ligase     degradation     nucleotide-binding leucine-rich repeat receptor     plant immunity     proteasome     protein stability     ubiquitination    

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Effects of resistin on insulin signaling in endothelial cells

Zhizhen LI, Fangping LI, Jianhong YE, Li YAN, Zuzhi FU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 136-140 doi: 10.1007/s11684-009-0029-2

Abstract: The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0-100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions ( < 0.05 control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation ( < 0.05 control) but had no effect on NO production and Akt phosphorylation levels. These findings suggested that resistin exerted an inhibitory effect on NO production by inhibiting insulin signaling and eNOS phosphorylation in endothelial cells.

Keywords： resistin     endothelium     nitric oxide     endothelial nitric oxide synthase     Akt-binding protein     mouse    

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Optimal Antibody Purification Strategies Using Data-Driven Models Article

Songsong Liu, Lazaros G. Papageorgiou

Engineering 2019, Volume 5, Issue 6,   Pages 1077-1092 doi: 10.1016/j.eng.2019.10.011

Abstract:

This work addresses the multiscale optimization of the purification processes of antibody fragments. Chromatography decisions in the manufacturing processes are optimized, including the number of chromatography columns and their sizes, the number of cycles per batch, and the operational flow velocities. Data-driven models of chromatography throughput are developed considering loaded mass, flow velocity, and column bed height as the inputs, using manufacturing-scale simulated datasets based on microscale experimental data. The piecewise linear regression modeling method is adapted due to its simplicity and better prediction accuracy in comparison with other methods. Two alternative mixed-integer nonlinear programming (MINLP) models are proposed to minimize the total cost of goods per gram of the antibody purification process, incorporating the data-driven models. These MINLP models are then reformulated as mixed-integer linear programming (MILP) models using linearization techniques and multiparametric disaggregation. Two industrially relevant cases with different chromatography column size alternatives are investigated to demonstrate the applicability of the proposed models.

Keywords： Antibody purification     Multiscale optimization     Antigen-binding fragment     Mixed-integer programming    

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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 376-380 doi: 10.1007/s11705-015-1531-5

Abstract: synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilutionFinally, a high IgG1 production of 780 mg/L was obtained from a batch culture.cell line, from gene to clone, could be completed within six month and the gene amplification improved protein

Keywords： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells

Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 204-211 doi: 10.1007/s11684-016-0443-1

Abstract: A positive clone of CD176 single-chain variable antibody fragment (scFv) was successfully screened from

Keywords： CD176     Thomsen-Friedenreich antigen     scFv     cancer     therapy     adhesion     metastasis    

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Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

Frontiers of Structural and Civil Engineering 2016, Volume 10, Issue 1,   Pages 112-120 doi: 10.1007/s11709-015-0322-x

Abstract: fly ash concrete, namely: 1) surface chloride content under tidal exposure condition; and 2) chloride bindingFirst, inconsistencies in surface chloride content and chloride binding models reported in literatureSecondly, to avoid such inconsistencies, surface chloride content and chloride binding models for fly

Keywords： binding isotherms     chloride ingress     concrete     fly ash     surface chloride content    

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Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma

Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 730-740 doi: 10.1007/s11684-019-0687-7

Abstract: GATA binding protein 3 (GATA3) and mismatch repair (MMR) deficiency contribute to the development of

Keywords： upper tract urothelial carcinoma     GATA binding protein 3     mismatch repair     microsatellite instability     prognosis    

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IgG N-Glycosylation Cardiovascular Age Tracks Cardiovascular Risk Beyond Calendar Age Article

Zhiyuan Wu, Zheng Guo, Yulu Zheng, Yutao Wang, Haiping Zhang, Huiying Pan, Zhiwei Li, Lois Balmer, Xia Li, Lixin Tao, Xiuhua Guo, Wei Wang

Engineering 2023, Volume 26, Issue 7,   Pages 99-107 doi: 10.1016/j.eng.2022.12.004

Abstract:

The use of an altered immunoglobulin G (IgG) N-glycan pattern as an inflammation metric hasHowever, the usable capacity of IgG N-glycosylation profiles for the risk stratification of cardiovascularThis study aimed to develop a cardiovascular aging index for tracking cardiovascular risk using IgG IgGdistance between GlyCage and calendar age independently indicates the cardiovascular risk, suggesting that IgG

Keywords： IgG N-glycosylation cardiovascular age     Cardiovascular aging     Immunoglobulin G     Glycosylation     Inflammation    

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reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 373-381 doi: 10.15302/J-FASE-2018210

Abstract: objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE bindingAdding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding abilityThe binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almostSDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial proteinwas probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.

Keywords： Aspergillus oryzae     egg allergy     egg white proteins     IgE-binding ability     ovomucoid    

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Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from

Yuan ZHANG,Yan ZHANG,Tao YU

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5,   Pages 666-674 doi: 10.1007/s11783-013-0608-y

Abstract: sediment samples were collected from various part of Taihu Lake to explore the spatial difference in the bindingThe ISE results showed that the exogenous DOM had higher binding ability than endogenous DOM, and DOMderived from aquatic macrophytes had a higher binding ability than that derived from algae.However, because of the frequent breakout of algae blooms, protein-like matters are also main componentTherefore, the metals bound by protein-like substances should be caused concern as protein-like substances

Keywords： binding ability     dissolved organic matters     fluorescence quenching     complex capacity     Taihu Lake    

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Title Author Date Type Operation

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

Journal Article

Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-

Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG

Journal Article

Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity

Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG

Journal Article

Control of lupus activity during pregnancy via the engagement of IgG sialylation: novel crosstalk betweenIgG sialylation and pDC functions

Journal Article

Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose

Dongmei WANG, Guodong JIA, Liang XU, Xiaoyan DONG, Yan SUN

Journal Article

Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

Journal Article

Effects of resistin on insulin signaling in endothelial cells

Zhizhen LI, Fangping LI, Jianhong YE, Li YAN, Zuzhi FU

Journal Article

Optimal Antibody Purification Strategies Using Data-Driven Models

Songsong Liu, Lazaros G. Papageorgiou

Journal Article

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Journal Article

CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells

Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao

Journal Article

Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

Journal Article

Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma

Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He

Journal Article

IgG N-Glycosylation Cardiovascular Age Tracks Cardiovascular Risk Beyond Calendar Age

Zhiyuan Wu, Zheng Guo, Yulu Zheng, Yutao Wang, Haiping Zhang, Huiying Pan, Zhiwei Li, Lois Balmer, Xia Li, Lixin Tao, Xiuhua Guo, Wei Wang

Journal Article

reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

Journal Article

Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from

Yuan ZHANG,Yan ZHANG,Tao YU

Journal Article