检索范围:
排序: 展示方式:
Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium
null
《医学前沿(英文)》 2016年 第10卷 第3期 页码 330-335 doi: 10.1007/s11684-016-0459-6
The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine N-methyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.
关键词: polycomb repressive complex Ezh2 expression inner ear cochlea development
《医学前沿(英文)》 2022年 第16卷 第4期 页码 637-650 doi: 10.1007/s11684-021-0864-3
关键词: apigenin aminoglycosides ototoxicity oxidative stress Nrf2 signaling pathway
Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 481-489 doi: 10.1007/s11684-016-0494-3
The tumor suppressor gene liver kinase B1 (LKB1), also called STK11, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice (LKB1Pax2 CKO mice) were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity (PCP) of hair cells. LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.
关键词: LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
《医学前沿(英文)》 2019年 第13卷 第6期 页码 690-704 doi: 10.1007/s11684-018-0638-8
关键词: TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing
Unidirectional and stage-dependent roles of Notch1 in Wnt-responsive Lgr5
Hui Jiang, Shan Zeng, Wenli Ni, Yan Chen, Wenyan Li
《医学前沿(英文)》 2019年 第13卷 第6期 页码 705-712 doi: 10.1007/s11684-019-0703-y
标题 作者 时间 类型 操作
Apigenin alleviates neomycin-induced oxidative damage via the Nrf2 signaling pathway in cochlear hair
期刊论文
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
期刊论文