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期刊论文 28

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Chemical probe systems for assessing liquid–liquid mixing efficiencies of reactors

《化学科学与工程前沿（英文）》 2023年 第17卷 第10期   页码 1323-1335 doi: 10.1007/s11705-022-2275-7

摘要： Liquid–liquid mixing, including homogeneous and heterogeneous mixing, widely exists in the chemical industry. How to quantitatively characterize the mixing performance is important for reactor assessment and development. As a convenient and direct method for mixing characterization, the chemical probe method uses some special test reactions to characterize the mixing results. Here, the working principle and selection requirements of this method are introduced, and some common chemical probe systems for homogeneous and heterogeneous mixing processes are reviewed. The characteristics and applications of these systems are illustrated. Finally, the development of the new system is proposed.

关键词： mixing     chemical probe     liquid–liquid     heterogeneous    

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A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 103-111 doi: 10.1007/s11705-021-2064-8

摘要： Uridine diphosphate (UDP)-glucuronosyltransferases (UGTs) are enzymes involved in the biotransformation of important endogenous compounds such as steroids, bile acids, and hormones as well as exogenous substances including drugs, environmental toxicants, and carcinogens. Here, a novel fluorescent probe BDMP was developed based on boron-dipyrromethene (BODIPY) with high sensitivity for the detection of UGT1A8. The glucuronidation of BDMP not only exhibited a red-emission wavelength (λex/λem = 500/580 nm), but also displayed an excellent UGT1A8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration. Based on this perfect biocompatibility and cell permeability, BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines (LoVo and HCT15) in real time. In addition, BDMP could also be used to visualize UGT1A8 in tumor tissues. These results suggested that BDMP is a promising molecular tool for the investigation of UGT1A8-mediated physiological function in humans.

关键词： UDP-glucuronosyltransferase 1A8     fluorescent probe     subtype selectivity     fluorescence imaging    

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A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 121-127 doi: 10.1007/s11705-021-2051-0

摘要： Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.

关键词： dual-function     fluorescent probe     labeling     photo-cross-linker     lipoylation modification     eraser     sirtuin    

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A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

《化学科学与工程前沿（英文）》 2023年 第17卷 第1期   页码 24-33 doi: 10.1007/s11705-022-2171-1

摘要： Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 μmol·L–1, significantly lower than that of the threshold value of 0.312 μmol·L–1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 μmol·L–1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

关键词： cinnamaldehyde     3-hydroxychromone derivative     hydrazine     fluorescent probe    

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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 92-102 doi: 10.1007/s11705-021-2063-9

摘要： Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases, such as genetic diseases and cancers. Therefore, the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition. Herein, we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN. The fluorophore was 2-styrene-1H-benzo[d]imidazole, and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA. In this design, MBI-CN conjugates to mitochondrial DNA without causing additional damages. Thus, MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment with mitochondrial DNA damage. Meanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA. Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA, it is a potentially effective detection tool for the real-time monitoring of mitochondrial DNA damage during cell apoptosis and initial assessment of cell apoptosis.

关键词： hydrolysis reaction     mitochondrial DNA damage     in situ hydrophobic environment trigger     fluorescence probe     apoptosis    

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A multi-probe micro-fabrication apparatus based on the friction-induced fabrication method

Zhijiang WU, Chenfei SONG, Jian GUO, Bingjun YU, Linmao QIAN

《机械工程前沿（英文）》 2013年 第8卷 第4期   页码 333-339 doi: 10.1007/s11465-013-0276-4

摘要：

A novel multi-probe micro-fabrication apparatus was developed based on the friction-induced fabrication method. The main parts of the apparatus include actuating device, loading system, and control system. With a motorized linear stage, the maximum fabrication area of 50 mm × 50 mm can be achieved, and the maximum sliding speed of probes can be as high as 10 mm/s. Through locating steel micro balls into indents array, the preparation of multi-probe array can be realized by a simple and low-cost way. The cantilever was designed as a structure of deformable parallelogram with two beams, by which the fabrication force can be precisely controlled. Combining the friction-induced scanning with selective etching in KOH solution, various micro-patterns were fabricated on Si(100) surface without any masks or exposure. As a low-cost and high efficiency fabrication device, the multi-probe micro-fabrication apparatus may encourage the development of friction-induced fabrication method and shed new light on the texture engineering.

关键词： friction-induced fabrication     silicon     surface texture     friction     multi-probe    

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Review of self-referenced measurement algorithms: Bridging lateral shearing interferometry and multi-probe

Dede ZHAI, Shanyong CHEN, Ziqiang YIN, Shengyi LI

《机械工程前沿（英文）》 2017年 第12卷 第2期   页码 143-157 doi: 10.1007/s11465-017-0432-3

摘要：

With the development of new materials and ultra-precision processing technology, the sizes of measured objects increase, and the requirements for machining accuracy and surface quality become more exacting. The traditional measurement method based on reference datum is inadequate for measuring a high-precision object when the quality of the reference datum is approximately within the same order as that of the object. Self-referenced measurement techniques provide an effective means when the direct reference-based method cannot satisfy the required measurement or calibration accuracy. This paper discusses the reconstruction algorithms for self-referenced measurement and connects lateral shearing interferometry and multi-probe error separation. In lateral shearing interferometry, the reconstruction algorithms are generally categorized into modal or zonal methods. The multi-probe error separation techniques for straightness measurement are broadly divided into two-point and three-point methods. The common features of the lateral shearing interferometry method and the multi-probe error separation method are identified. We conclude that the reconstruction principle in lateral shearing interferometry is similar to the two-point method in error separation on the condition that no yaw error exists. This similarity may provide a basis or inspiration for the development of both classes of methods.

关键词： self-referenced measurement     lateral shearing interferometry     multi-probe error separation     surface metrology    

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Near-infrared fluorescent probe for fast track of cyclooxygenase-2 in Golgi apparatus in cancer cells

Bhaskar Gurram, Miao Li, Jiangli Fan, Jingyun Wang, Xiaojun Peng

《化学科学与工程前沿（英文）》 2020年 第14卷 第1期   页码 41-52 doi: 10.1007/s11705-019-1796-1

摘要： Cyclooxygenase-2 (COX-2) has been used as an excellent traceable biomarker, and exists maximally in Golgi apparatus (Cancer cells). Celecoxib (CCB) is a selective inhibitor for COX-2, and has been used as one of non-steroidal anti-inflammatory drug. Herein we report the conjugation of nile blue (NB) with CCB via a six-carbon linkage to form a fluorescence probe NB-C6-CCB for the detection of COX-2. NB-C6-CCB displays strong fluorescence with the emission peak centered at near-infrared wavelength (700 nm) in tumor cells or tumor tissues with high expression of COX-2. Importantly, NB-C6-CCB can discriminate cancer cells (MCF-7) fluorescence intensity from normal ones (COS-7) in the co-culture medium under confocal microscope. Subcellular localization of the NB-C6-CCB preferentially points to the Golgi apparatus and increases the fluorescent intensity. The competitive analysis (with CCB) and Native-PAGE analysis confirmed that NB-C6-CCB shows selective binding affinity towards COX-2 enzyme. Competitive analysis with CCB (flow cytometry assay) revealed the fluorescence intensity fluctuation due to pretreatment of CCB with different concentrations, indicating that the NB-C6-CCB is a precise or sensitive probe for the COX-2. Tumor tissue (depth: 500 µm), organs and mice imaging tests show excellent near-infrared visualization, specific localization and identification of tumors.

关键词： cyclooxygenase-2     nile blue     CCB     Golgi apparatus     NIR imaging    

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Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

null

《医学前沿（英文）》 2018年 第12卷 第1期   页码 23-33 doi: 10.1007/s11684-017-0607-7

摘要：

Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunity for microbial genome. Consequently, considerable genetic diversity encoded by bacterial genomes and among the strains in the same species has been revealed. In recent years, genome sequencing techniques and bioinformatics have developed rapidly, which has resulted in transformation and expedited the application of strategy and methodology for bacterial genome comparison used in dissection of infectious disease epidemics. Bacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements of epidemiological study in disease control. In this review, we outline the significance and summarize the roles of bacterial genome sequencing in the context of bacterial disease control and prevention. We discuss the applications of bacterial genome sequencing in outbreak detection, source tracing, transmission mode discovery, and new epidemic clone identification. Wide applications of genome sequencing and data sharing in infectious disease surveillance networks will considerably promote outbreak detection and early warning to prevent the dissemination of bacterial diseases.

关键词： genome sequencing     genomic epidemiology     bacteria     surveillance     infectious diseases    

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approach for fabricating photoluminescent carbon dots and its potential application as an efficient sensor probe

《化学科学与工程前沿（英文）》 2023年 第17卷 第5期   页码 536-547 doi: 10.1007/s11705-022-2239-y

摘要： The past decade has witnessed a variety of members of the carbon family along with exposure of carbon dots due to their magnificent properties in sensing, bioimaging, catalytic applications, biomedical fields, and so on. Herein, we report the simple hydrothermal method to fabricate photoluminescent doped carbon quantum dots for the detection of noxious lead(II) ions. Lead(II) ion is very venomous for both the environment and human health for which its detection is demanded area in the research field. The as-prepared carbon dots show excellent photostability, low toxicity and significant photoluminescence properties along with good water solubility. Along with these properties, carbon dots have a quantum yield of approximately 15%. In the practical field of application, these carbon dots have been used as sensing probes for the detection of lead(II) ions with a detection limit of 60 nmol·L–1. The fluorescence intensity of carbon dots was remarkably quenched in the presence of the lead(II) ion selectively among all the tested metal ions. Furthermore, we have studied the Stern–Volmer relationship for lead(II) quenching along with the explanation of the probable quenching mechanism. Ability of the doped carbon dots in heavy metal ions sensing in an environmental sample was demonstrated.

关键词： carbon dots     fluorescence     heavy metal sensing     practical application     photoluminescence    

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大面积扫描探针显微镜研制

路小波,陆祖宏,周庆,王国著

《中国工程科学》 2004年 第6卷 第9期   页码 50-55

摘要：

介绍了一种大面积扫描探针显微镜。仪器采用大范围扫描与高精度微动扫描相分离、X-Y扫描与纵向检测相分离的方案，同时具备较大扫描范围和较高的分辨率，扫描重复性好，大面积扫描范围220mm×290mm×60mm，重复性2～3 μm，并可实现探针的自动逼近、光学显微镜自动聚焦和图像自动拼接。

关键词： 扫描探针显微镜     自动逼近     自动聚焦     图像拼接    

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Traffic services for vehicles: the process from receiving raw probe data to space-time diagrams and the

Markus AUER, Hubert REHBORN, Sven-Eric MOLZAHN, Micha KOLLER

《工程管理前沿（英文）》 2017年 第4卷 第4期   页码 490-497 doi: 10.15302/J-FEM-2017008

摘要： Today, large quantities of vehicle data (FCD: floating car data) are widely used by traffic service providers to create and broadcast traffic states in road networks. As a first processing step, all raw position data received from Global Positioning Systems (GPS) have to be map matched in a digital road map. The technical aspects of such a matching process for GPS data are described in this report. After the matching process, space-time-diagrams are created of the probe data showing traffic situation details over space and time. Various examples illustrate how traffic service quality depends on the number of matched GPS raw data; it will be stated that when 2% of connected vehicles in the total traffic flow are sending their GPS data in shorter time intervals, a high quality and precise reconstruction of the current traffic phases is achieved. Traffic reconstruction is followed by a translation into traffic information messages, which can be sent and used in vehicle navigation systems for driver information and dynamic route guidance.

关键词： floating car data     map matching     three phase traffic theory     traffic reconstruction     traffic service quality     navigation systems    

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A simple, azulene-based colorimetric probe for the detection of nitrite in water

Lloyd C. Murfin, Carlos M. López-Alled, Adam C. Sedgwick, Jannis Wenk, Tony D. James, Simon E. Lewis

《化学科学与工程前沿（英文）》 2020年 第14卷 第1期   页码 90-96 doi: 10.1007/s11705-019-1790-7

摘要： We describe the synthesis and evaluation of an azulene-based chemodosimeter for nitrite. The probe was found to undergo two distinct color changes upon introduction aqueous nitrite ion. A near-instant formation of a grey color provides a qualitative indication of the presence of nitrite, followed by the formation of a deep-yellow/orange color, the endpoint from which quantitative data can be derived. The azulene probe exhibits 1:1 stoichiometry of reaction with nitrite in water, and is selective for nitrite over other anions. The azulene probe was applied to determine nitrite content in cured meat, and compared with the British Standard testing procedure (Griess test). The value obtained from the azulene-based probe agreed closely with the standard test. Our procedure only requires the preparation of one standard solution, instead of the three required for the standard Griess test.

关键词： azulene     nitrite     diazoquinone    

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

《化学科学与工程前沿（英文）》 2018年 第12卷 第2期   页码 311-314 doi: 10.1007/s11705-017-1697-0

摘要： A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

关键词： fragmentation     NCOU1     NTR     nitroreductase     nitrobenzyl-umbelliferone    

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Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 106-115 doi: 10.15302/J-FASE-2017133

摘要： A series of dehydroabietic acid-based diarylamines have been synthesized in order to investigate their fluorescent properties, photostability, cell toxicity and fluorescence imaging. The geometries as well as their molecular properties were optimized at the B3LYP/6-31G* level using Gaussian 03. The results indicate that molecular geometry, HOMO and LUMO energies, and energy gaps are important to predict absorption and fluorescent properties. Five of the compounds can be effectively taken up by human cervical carcinoma, human hepatocellular carcinoma SMMC-7721, human gastric cancer SGC-7901 and human lung adenocarcinoma A549 cells and strong blue fluorescent signals are detected in these cells. These compounds are potential candidates for fluorescent probes in biological diagnosis.

关键词： dehydroabietic acid-based diarylamine     DFT study     fluorescent probe     in vitro imaging    

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标题 作者 时间 类型 操作

Chemical probe systems for assessing liquid–liquid mixing efficiencies of reactors

期刊论文

A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

期刊论文

A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

期刊论文

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

期刊论文

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

期刊论文

A multi-probe micro-fabrication apparatus based on the friction-induced fabrication method

Zhijiang WU, Chenfei SONG, Jian GUO, Bingjun YU, Linmao QIAN

期刊论文

Review of self-referenced measurement algorithms: Bridging lateral shearing interferometry and multi-probe

Dede ZHAI, Shanyong CHEN, Ziqiang YIN, Shengyi LI

期刊论文

Near-infrared fluorescent probe for fast track of cyclooxygenase-2 in Golgi apparatus in cancer cells

Bhaskar Gurram, Miao Li, Jiangli Fan, Jingyun Wang, Xiaojun Peng

期刊论文

Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

null

期刊论文

approach for fabricating photoluminescent carbon dots and its potential application as an efficient sensor probe

期刊论文

大面积扫描探针显微镜研制

路小波,陆祖宏,周庆,王国著

期刊论文

Traffic services for vehicles: the process from receiving raw probe data to space-time diagrams and the

Markus AUER, Hubert REHBORN, Sven-Eric MOLZAHN, Micha KOLLER

期刊论文

A simple, azulene-based colorimetric probe for the detection of nitrite in water

Lloyd C. Murfin, Carlos M. López-Alled, Adam C. Sedgwick, Jannis Wenk, Tony D. James, Simon E. Lewis

期刊论文

A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B. P. Elmes, Tony D. James

期刊论文

Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

期刊论文