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Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma and
《医学前沿(英文)》 2023年 第17卷 第1期 页码 132-142 doi: 10.1007/s11684-022-0938-x
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《医学前沿(英文)》 2016年 第10卷 第1期 页码 41-51 doi: 10.1007/s11684-016-0429-z
Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme linked to tumor progression and a novel interacting partner of breast cancer 1, early-onset (BRCA1). However, the role of MID2 in breast cancer remains unknown. This study investigated the expression, prognostic value, and role of MID2 in breast cancer. The expression of MID2 mRNA and protein was significantly upregulated in breast cancer tissue and established cell lines compared with that in normal breast epithelial cells and paired adjacent non-tumor tissue (P<0.001). Immunohistochemical analysis demonstrated that MID2 was overexpressed in 272 of 284 (95.8%) paraffin-embedded, archived breast cancer tissue. Moreover, MID2 expression increased with advanced clinical stage (P<0.001). High MID2 expression was significantly associated with advanced clinical stages and T, N, and M staging (all P<0.05). Univariate and multivariate analyses indicated that high MID2 expression was an independent prognostic factor for poor overall survival in the entire cohort (93.73 vs. 172.1 months; P<0.001, log-rank test) and in subgroups with stages Tis+ I+ II and III+ IV. Furthermore, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide colony formation, and anchorage-independent growth ability assays were conducted. Results showed that siRNA silencing of MID2 expression significantly reduced MCF-7 and MDA-MB-231 cell proliferation in vitro and blocked the growth of MDA-MB-231 cell xenograft tumors in vivo (P<0.05). This study indicated that MID2 may be a novel prognostic marker and interventional target in breast cancer.
关键词: breast cancer MID2 proliferation overall survival xenograft
The MYC transcription factor network: balancing metabolism, proliferation and oncogenesis
null
《医学前沿(英文)》 2018年 第12卷 第4期 页码 412-425 doi: 10.1007/s11684-018-0650-z
Transcription factor networks have evolved in order to control, coordinate, and separate, the functions of distinct network modules spatially and temporally. In this review we focus on the MYC network (also known as the MAX-MLX Network), a highly conserved super-family of related basic-helix-loop-helix-zipper (bHLHZ) proteins that functions to integrate extracellular and intracellular signals and modulate global gene expression. Importantly the MYC network has been shown to be deeply involved in a broad spectrum of human and other animal cancers. Here we summarize molecular and biological properties of the network modules with emphasis on functional interactions among network members. We suggest that these network interactions serve to modulate growth and metabolism at the transcriptional level in order to balance nutrient demand with supply, to maintain growth homeostasis, and to influence cell fate. Moreover, oncogenic activation of MYC and/or loss of a MYC antagonist, results in an imbalance in the activity of the network as a whole, leading to tumor initiation, progression and maintenance.
Meng SONG, Wei SUN
《能源前沿(英文)》 2022年 第16卷 第1期 页码 64-73 doi: 10.1007/s11708-021-0732-5
关键词: thermostatically controlled load demand response renewable energy power system operation
《医学前沿(英文)》 2021年 第15卷 第6期 页码 942-942 doi: 10.1007/s11684-021-0876-z
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 72-80 doi: 10.15302/J-FASE-2016089
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《医学前沿(英文)》 2018年 第12卷 第3期 页码 289-300 doi: 10.1007/s11684-017-0550-7
Silver-containing preparations are widely used in the management of skin wounds, but the effects of silver ions on skin wound healing remain poorly understood. This study investigated the effects of silver ions (Ag+) on the proliferation of human skin keratinocytes (HaCaT) and the production of intracellular reactive oxygen species (ROS). After treating HaCaT cells with Ag+and/or the active oxygen scavenger N-acetyl cysteine (NAC), cell proliferation and intracellular ROS generation were assessed using CCK-8 reagent and DCFH-DA fluorescent probe, respectively. In addition, 5-bromo-2-deoxyUridine (BrdU) incorporation assays, cell cycle flow cytometry, and proliferating cell nuclear antigen (PCNA) immunocytochemistry were conducted to further evaluate the effects of sub-cytotoxic Ag+ concentrations on HaCaT cells. The proliferation of HaCaT cells was promoted in the presence of 10−6 and 10−5 mol/L Ag+ at 24, 48, and 72 h. Intracellular ROS generation also significantly increased for 5–60 min after exposure to Ag+. The number of BrdU-positive cells and the presence of PCNA in HaCaT cells increased 48 h after the addition of 10−6 and 10−5 mol/L Ag+, with 10−5 mol/L Ag+ markedly increasing the cell proliferation index. These effects of sub-cytotoxic Ag+ concentrations were repressed by 5 mmol/L NAC. Our results suggest that sub-cytotoxic Ag+ concentrations promote the proliferation of human keratinocytes and might be associated with a moderate increase in intracellular ROS levels. This study provides important experimental evidence for developing novel silver-based wound agents or dressings with few or no cytotoxicity.
关键词: ionic silver human keratinocyte cell proliferation reactive oxygen species active oxygen scavenger NAC
Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface
Linlin LIANG, Bei XU, Guijin ZHU
《医学前沿(英文)》 2009年 第3卷 第2期 页码 220-226 doi: 10.1007/s11684-009-0037-2
关键词: gonadotropin-releasing hormone ovarian reserve embryo developmental ability ovarian surface epithelium
ZHAN Rong, YU Qinghong, HUANG Haobo
《医学前沿(英文)》 2008年 第2卷 第4期 页码 356-360 doi: 10.1007/s11684-008-0068-0
关键词: VEGF expression expression variation culture supernatant labeling concentration
Zfyve16 regulates the proliferation of B-lymphoid cells
Xuemei Zhao, Donghe Li, Qingsong Qiu, Bo Jiao, Ruihong Zhang, Ping Liu, Ruibao Ren
《医学前沿(英文)》 2018年 第12卷 第5期 页码 559-565 doi: 10.1007/s11684-017-0562-3
Zfyve16 (a.k.a. endofin or endosome-associated FYVE-domain protein), a member of the FYVE-domain protein family, is involved in endosomal trafficking and in TGF-β, BMP, and EGFR signaling. The FYVE protein SARA regulates the TGF-β signaling pathway by recruiting Smad2/3 and accelerating their phosphorylation, thereby altering their susceptibility to TGF-β-mediated T cell suppression. Zfyve16 binds to Smad4 and their binding affects the formation of Smad2/3-Smad4 complex in TGF-β signaling. However, the in vivo function of Zfyve16 remains unknown. In this study, we generated a Zfyve16 knockout mouse strain (Zfyve16KO) and examined its hematopoietic phenotypes and hematopoietic reconstruction ability. The proportion of T cells in the peripheral blood of Zfyve16KO mice increases compared with that in wild-type mice. This finding is consistent with the role of Zfyve16 in facilitating TGF-β signaling. Unpredictably, B cell proliferation is inhibited in Zfyve16KO mice. The proliferation potential of Zfyve16KO B-lymphoid cells also significantly decreases in vitro. These results suggest that Zfyve16 inhibits the proliferation of T cells, possibly through the TGF-β signaling, but upregulates the proliferation of B-lymphoid cells.
《医学前沿(英文)》 2022年 第16卷 第5期 页码 784-798 doi: 10.1007/s11684-021-0911-0
关键词: uveal melanoma mutant GNAQ/11 palmitoylation BCL2 combination target therapy
Effect on proliferation and apoptosis of T24 cell lines via silencing DNMT1 with RNA interference
ZHANG Shilong, ZENG Fuqing, PENG Shibo, WANG Liang
《医学前沿(英文)》 2008年 第2卷 第4期 页码 374-379 doi: 10.1007/s11684-008-0072-4
Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts
Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 379-383 doi: 10.1007/s11684-009-0073-y
关键词: p38 regulated/activated protein kinase gene knockout cell cycle cell proliferation
Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang
《医学前沿(英文)》 2021年 第15卷 第1期 页码 91-100 doi: 10.1007/s11684-020-0778-5
关键词: congenital heart disease Gene Expression Omnibus lncRNA SAP30-2:1 cell proliferation RNA immunoprecipitation HAND2
NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
《医学前沿(英文)》 2019年 第13卷 第6期 页码 646-657 doi: 10.1007/s11684-018-0643-y
关键词: androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy proliferation
标题 作者 时间 类型 操作
is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation
null
期刊论文
Applications of thermostatically controlled loads for demand response with the proliferation of variable
Meng SONG, Wei SUN
期刊论文
is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation
期刊论文
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
期刊论文
Sub-cytotoxic concentrations of ionic silver promote the proliferation of human keratinocytes by inducing
null
期刊论文
Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface
Linlin LIANG, Bei XU, Guijin ZHU
期刊论文
Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the
ZHAN Rong, YU Qinghong, HUANG Haobo
期刊论文
Zfyve16 regulates the proliferation of B-lymphoid cells
Xuemei Zhao, Donghe Li, Qingsong Qiu, Bo Jiao, Ruihong Zhang, Ping Liu, Ruibao Ren
期刊论文
Palmitoylation of GNAQ/11 is critical for tumor cell proliferation and survival in GNAQ/11-mutant uveal
期刊论文
Effect on proliferation and apoptosis of T24 cell lines via silencing DNMT1 with RNA interference
ZHANG Shilong, ZENG Fuqing, PENG Shibo, WANG Liang
期刊论文
Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts
Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,
期刊论文
Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation
Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang
期刊论文