Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 7

会议视频 1

年份

2023 1

2022 2

2019 1

2018 1

2017 1

2015 1

关键词

MCF-7细胞球 1

加密 1

微孔板芯片 1

抗冻水凝胶 1

水凝胶 1

组织工程 1

细胞打印 1

聚集诱导发光 1

解密 1

防伪 1

展开︾

检索范围：

排序：展示方式：

Chemically triggered life control of “smart” hydrogels through click and declick reactions

《化学科学与工程前沿（英文）》 2022年第16卷第9期页码 1399-1406 doi: 10.1007/s11705-022-2149-z

摘要： The degradation of polymeric materials is recognized as one of the goals to be fulfilled for the sustainable economy. In this study, a novel methodology was presented to synthesize multiple highly cross-linked polymers (i.e., hydrogels) through amine–thiol scrambling under mild conditions. Amine-terminated poly(ethylene glycol) (PEG-NH₂) was reacted with the representative conjugate acceptors to synthesize hydrogels in organic and aqueous solutions, respectively. The materials above exhibited high water-swelling properties, distributed porous structures, as well as prominent mechanical strengths. It is noteworthy that the mentioned hydrogels could be degraded efficiently in hours to release the original coupling partner, which were induced by ethylene diamine at ambient temperature through amine-amine metathesis. The recovered PEG-NH₂ reagent could be employed again to regenerate hydrogels. Due to the multiple architectures and functions in polymeric synthesis, degradation and regeneration, a new generation of “smart” materials is revealed.

关键词： hydrogels degradation synthesis regeneration

HTML PDF 收藏

Molecular tailoring to improve polypyrrole hydrogels’ stiffness and electrochemical energy storage capacity

Evelyn Chalmers, Yi Li, Xuqing Liu

《化学科学与工程前沿（英文）》 2019年第13卷第4期页码 684-694 doi: 10.1007/s11705-019-1817-0

摘要： This research looks at ways of tailoring and improving the stiffness of polypyrrole hydrogels for use as flexible supercapacitor electrodes. Molecules providing additional cross-linking between polypyrrole chains are added post-polymerisation but before gelation, and are found to increase gel stiffness by up to 600%, with the degree of change dependent on reactant type and proportion. It was also found that addition of phytic acid led to an increase in pseudocapacitive behaviour of the hydrogel, and thus a maximum specific capacitance of 217.07 F·g could be achieved. This is an increase of 140% compared to pristine polypyrrole hydrogels produced by this method.

关键词： supercapacitor polypyrrole hydrogel strengthening electrochemical

HTML PDF 收藏

Preparation of hydrogels with uniform and gradient chemical structures using dialdehyde cellulose and

Peiwen Liu, Carsten Mai, Kai Zhang

《化学科学与工程前沿（英文）》 2018年第12卷第3期页码 383-389 doi: 10.1007/s11705-018-1718-7

摘要：

Hydrogels with precisely designed structures represent promising materials with a broad application spectrum, such as for sensor, tissue engineering and biomimetic technology. However, with highly reactive compounds, the preparation of hydrogels still needs an efficient approach for desired distribution of each component within hydrogels. In addition, a method for in situ preparation of gradient hydrogels is still lacking. Herein, we report the formation of hydrogels with either uniform or gradient internal structures via a novel, simple but very efficient method by aerating ammonia gas (NH₃ gas) into the solution of dialdehyde cellulose (DAC) and a diamine. As-prepared hydrogels exhibited uniform microscopic and chemical structure or gradient distribution of functional groups. Due to lots of aldehyde groups on DAC chains, functional hydrogels can be prepared by using diverse diamines. For instance, hydrogels prepared by using 1,6-hexanediamine as a cross-linker were responsive to pH values. Moreover, this controllable process of aerating NH₃ gas allows the in situ formation of gradient hydrogels; for instance, by using cyanamide as a reaction counterpart, gradient hydrogels with gradient distributions of cyanide groups were prepared.

关键词： hydrogel uniform gradient dialdehyde cellulose ammonia gas diamine

HTML PDF 收藏

Enzyme-instructed self-assembly of peptides containing phosphoserine to form supramolecular hydrogels

Jie Zhou, Xuewen Du, Jiaqing Wang, Natsuko Yamagata, Bing Xu

《化学科学与工程前沿（英文）》 2017年第11卷第4期页码 509-515 doi: 10.1007/s11705-017-1613-7

摘要： Enzyme-instructed self-assembly (EISA) offers a facile approach to explore the supramolecular assemblies of small molecules in cellular milieu for a variety of biomedical applications. One of the commonly used enzymes is phosphatase, but the study of the substrates of phosphatases mainly focuses on the phosphotyrosine containing peptides. In this work, we examine the EISA of phosphoserine containing small peptides for the first time by designing and synthesizing a series of precursors containing only phosphoserine or both phosphoserine and phosphotyrosine. Conjugating a phosphoserine to the -terminal of a well-established self-assembling peptide backbone, (naphthalene-2-ly)-acetyl-diphenylalanine (NapFF), affords a novel hydrogelation precursor for EISA. The incorporation of phosphotyrosine, another substrate of phosphatase, into the resulting precursor, provides one more enzymatic trigger on a single molecule, and meanwhile increases the precursors’ propensity to aggregate after being fully dephosphorylated. Exchanging the positions of phosphorylated serine and tyrosine in the peptide backbone provides insights on how the specific molecular structures influence self-assembling behaviors of small peptides and the subsequent cellular responses. Moreover, the utilization of D-amino acids largely enhances the biostability of the peptides, thus providing a unique soft material for potential biomedical applications.

关键词： enzyme-instructed self-assembly phosphoserine phosphatase supramolecular hydrogel

HTML PDF 收藏

具有聚集诱导发光活性的抗冻水凝胶用于低温环境下多级信息的加密和解密 Article

睢晓洁, 汪晓东, 蔡诚诚, 马君怡, 杨静, 张雷

《工程（英文）》 2023年第23卷第4期页码 82-89 doi: 10.1016/j.eng.2022.03.021

摘要：

抗冻水凝胶可以在零下温度环境中调节内部水分子的冻结行为，从而保持其优异特性（如智能响应性和液体运输）以及拓展其在寒冷条件下的应用。本文开发了一系列具有聚集诱导发光（AIE）活性的抗冻水凝胶，可实现在零下温度的信息加密和解密。通过改变水凝胶内的甜菜碱含量，该水凝胶可呈现出不同的冻结温度（T_f）。当温度高于/低于T_f时，编码于水凝胶内的AIE荧光分子不发射/发射荧光，从而可以实现信息的加密和解密。此外，通过调控水凝胶的冻结程序或者在水凝胶内原位引入具有光热效应的硫化铜纳米颗粒并结合特定的照射条件，可以实现信息的多级加密和解密，从而增强信息的安全性。最后，由于解密的信息图案对温度波动具有不可逆性，因此该具有AIE活性的抗冻水凝胶可作为无需外界供能的防伪标签，用于实时和可视化监测冷冻运输（−80 ℃）过程中生物样本（如间充质干细胞和红细胞）的活性。

关键词：抗冻水凝胶聚集诱导发光加密解密防伪

HTML PDF 收藏

A Double-Layer Polysaccharide Hydrogel (DPH) for the Enhanced Intestine-Targeted Oral Delivery of Probiotics

Wen-Can Huang,Wenjie Wang,Wei Wang,Yanan Hao,Changhu Xue,Xiangzhao Mao,

《工程（英文）》 doi: 10.1016/j.eng.2023.05.024

摘要： Transplantation of probiotics to the intestine can positively regulate the gut microbiota, thereby promoting the immune system and treating various diseases. However, the harsh gastrointestinal environment and short retention time in the gastrointestinal tract significantly limit the bioavailability and intestinal colonization of probiotics. Herein, we present a double-layer polysaccharide hydrogel (DPH) in the form of a double-layer structure composed of a carboxymethyl cellulose (CMCL) supramolecular inner layer and a dialdehyde alginate (DAA) cross-linked carboxymethyl chitosan (CMCS) outer layer. This double-layer structure allows DPH to encapsulate and deliver probiotics in a targeted manner within the body. In the stomach, the cage structure of the DPH is closed, and the outer layer absorbs surrounding liquids to form a barrier to protect the probiotics from gastric fluids. In the intestine, the cage structure opens and disintegrates, releasing the probiotics. Thus, DPH endows probiotics with excellent intestine-targeted delivery, improved oral bioavailability, enhanced gastrointestinal tract tolerance, and robust mucoadhesion capacity. The encapsulated probiotics exhibit almost unchanged bioactivity in the gastrointestinal tract before release, as well as improved oral delivery. In particular, probiotics encapsulated by DPH exhibit 100.1 times higher bioavailability and 10.6 times higher mucoadhesion than free probiotics in an animal model 48 h post-treatment. In addition, with a remarkable ability to survive and be retained in the intestine, probiotics encapsulated by DPH show excellent in vitro and in vivo competition with pathogens. Notably, DAA-mediated dynamic crosslinking not only maintains the overall integrity of the hydrogels but also controls the release timing of the probiotics. Thus, it is expected that encapsulated substances (probiotics, proteins, etc.) can be delivered to specific sites of the intestinal tract by means of DPH, by controlling the dynamic covalent crosslinking.

关键词： Polysaccharides Chitosan Hydrogels Oral delivery Intestine-targeted

HTML PDF 收藏

基于细胞打印的三维MCF-7乳腺癌细胞球高通量构建研究 Article

凌楷,黄国友,刘俊聪,张晓慧,马玉菲,卢天健,徐峰

《工程（英文）》 2015年第1卷第2期页码 269-274 doi: 10.15302/J-ENG-2015062

摘要：

基于细胞球的体外组织模型在生理病理机理和药物筛选方面具有重要的研究意义和广阔的应用前景，利用微纳生物制造技术可控构建细胞球已吸引了越来越多研究者的关注。微孔板法作为一种简单易行的细胞球构建方法经常被研究者采用，但目前的微孔板法存在结构制备和可控性不足、细胞种植不均匀以及细胞容易流失等问题。本文利用自行搭建的细胞打印技术制备载细胞的明胶微凝胶作为模板，构建了带有凹面微孔阵列的聚乙二醇二甲基丙烯酸酯水凝胶微孔板芯片并原位形成了乳腺癌细胞球。本方法操作灵活、可控性好，避免了细胞种植不均匀和细胞流失等问题，在组织工程、再生医学以及药物筛选等研究具有重要的应用价值。

关键词： MCF-7细胞球细胞打印水凝胶微孔板芯片