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Regulatory mechanism and functional analysis of
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《医学前沿（英文）》 2017年 第11卷 第1期 页码 87-96 doi: 10.1007/s11684-016-0469-4
S100A9, a calcium-binding protein, participates in the inflammatory process and development of various tumors, thus attracting much attention in the field of cancer biology. This study aimed to investigate the regulatory mechanism of and its function involvement in APL. We used real-time quantitative PCR to determine whether PML/RARα affects the expression of in NB4 and PR9 cells upon ATRA treatment. ChIP-based PCR and dual-luciferase reporter assay system were used to detect how PML/RARα and PU.1 regulate promoter activity. CCK-8 assay and flow cytometry were employed to observe the viability and apoptosis of NB4 cells when was overexpressed. Results showed that was an ATRA-responsive gene, and PML/RARα was necessary for the ATRA-induced expression of in APL cells. In addition, PU.1 could bind to the promoter of , especially when treated with ATRA in NB4 cells, and promote its activity. More importantly, overexpression of induced the apoptosis of NB4 cells and inhibited cell growth. Collectively, our data indicated that PML/RARα and PU.1 were necessary for the ATRA-induced expression of in APL cells. Furthermore, promoted apoptosis in APL cells and affected cell growth.
关键词： S100A9 S100A9 PU.1 PML/RARα ATRA APL
Insight into the role of cholesterol in modulation of morphology and mechanical properties of CHO-K1
Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen
《化学科学与工程前沿（英文）》 2019年 第13卷 第1期 页码 98-107 doi: 10.1007/s11705-018-1775-y
Cholesterol plays a significant role in the organization of lipids and modulation of membrane dynamics in mammalian cells. However, the effect of cholesterol depletion on the eukaryotic cell membranes seems controversial. In this study, the effects of cholesterol on the topography and mechanical behaviors of CHO-K1 cells with manipulated membrane cholesterol contents were investigated by atomic force microscopy (AFM) technique. Here, we found that the depletion of cholesterol in cell membranes could increase the membrane stiffness, reduce the cell height as well as promote cell retraction and detachment from the surface, whereas the cholesterol restoration could reverse the effect of cholesterol depletion on the membrane stiffness. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. This research demonstratede importance of cholesterol in regulating the dynamics of cytoskeleton-mediated processes. AFM technique offers excellent advantages in the dynamic monitoring of the change in membranes mechanical properties and behaviors during the imaging process. This promising technology can be utilized in studying the membrane properties and elucidating the underlying mechanism of distinct cells in the near-native environment.
关键词： cholesterol methyl-β-cyclodextrin atomic force microscopy Young’s modulus CHO-K1 cell
China ADS sub-critical experimental assembly—Venus-1 and preliminary experiment
SHI Yongqian, XIA Pu, LUO Zhanglin, ZHAO Zhixiang, DING Dazhao, ZHU Qinfu, XIA Haihong, LI Jigen, ZHANG
《能源前沿（英文）》 2007年 第1卷 第2期 页码 150-157 doi: 10.1007/s11708-007-0019-5
关键词： coupled D-D reaction dynamic pulsed neutron sub-critical assembly
基于场景自适应概念学习的无监督目标检测 Research Articles
浦世亮1,赵暐1,陈伟杰1,杨世才1,谢迪1,潘云鹤2
《信息与电子工程前沿（英文）》 2021年 第22卷 第5期 页码 615-766 doi: 10.1631/FITEE.2000567
基于专家示教聚类经验池的高效深度强化学习 Research Article
王士珉1,赵彬琦1,张政锋1,张军平1,浦剑2
《信息与电子工程前沿（英文）》 2023年 第24卷 第11期 页码 1541-1556 doi: 10.1631/FITEE.2300084
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《医学前沿（英文）》 2014年 第8卷 第2期 页码 217-226 doi: 10.1007/s11684-014-0326-2
This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>104 copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.
关键词： hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations
自监督脓毒症治疗推荐算法 Research Articles
朱思涵1,浦剑2
《信息与电子工程前沿（英文）》 2021年 第22卷 第7期 页码 926-939 doi: 10.1631/FITEE.2000127
基于脉冲分忆抗桥电路的分数阶记忆性神经突触加权 Research Articles
蒲亦非1,余波1,2,何秋燕1,袁晓3
《信息与电子工程前沿（英文）》 2021年 第22卷 第6期 页码 862-876 doi: 10.1631/FITEE.2000085
Characteristics of two transferable aminoglycoside resistance plasmids in
Chengjun Pu, Xiaoyan Gong, Ying Sun
《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1119-2
pRKZ3 is a non-conjugative IncQ plasmid, while pKANJ7 is a conjugative IncX plasmid. The optimal mating time of pKANJ7 varied under different conditions. Both of the two transferable ARPs had little impact on the growth of their hosts. A relatively high level of fitness cost was observed for pKANJ7. The fitness cost of ARPs depended on their hosts.
关键词： Escherichia coli Characteristics Aminoglycoside resistance plasmids Transfer Persistence
Game theoretic analysis of environmental impact assessment system in China
CHENG Hongguang, PU Xiao, GONG Li, QI Ye
《环境科学与工程前沿（英文）》 2007年 第1卷 第4期 页码 448-453 doi: 10.1007/s11783-007-0071-8
陈江山1,2,胡予濮1,梁红梅2,高雯3
《信息与电子工程前沿（英文）》 2021年 第22卷 第2期 页码 141-286 doi: 10.1631/FITEE.1900318
New Challenges, New Opportunities and New Measures for Oil and Gas Cooperation in the Belt and Road
Yu-pu Wang,He Liu
《工程管理前沿（英文）》 2016年 第3卷 第4期 页码 301-313 doi: 10.15302/J-FEM-2016055
关键词： the Belt and Road oil & gas cooperation new challenges new opportunities new measures
CORMAND2——针对工业机器人的欺骗攻击 Article
Hongyi Pu,Liang He,Peng Cheng,Jiming Chen,Youxian Sun
《工程（英文）》 2024年 第32卷 第1期 页码 186-202 doi: 10.1016/j.eng.2023.01.013
Industrial robots are becoming increasingly vulnerable to cyber incidents and attacks, particularly with the dawn of the Industrial Internet-of-Things (IIoT). To gain a comprehensive understanding of these cyber risks, vulnerabilities of industrial robots were analyzed empirically, using more than three million communication packets collected with testbeds of two ABB IRB120 robots and five other robots from various Original Equipment Manufacturers (OEMs). This analysis, guided by the confidentiality–integrity–availability (CIA) triad, uncovers robot vulnerabilities in three dimensions: confidentiality, integrity, and availability. These vulnerabilities were used to design Covering Robot Manipulation via Data Deception (CORMAND2), an automated cyber–physical attack against industrial robots. CORMAND2 manipulates robot operation while deceiving the Supervisory Control and Data Acquisition (SCADA) system that the robot is operating normally by modifying the robot’s movement data and data deception. CORMAND2 and its capability of degrading the manufacturing was validated experimentally using the aforementioned seven robots from six different OEMs. CORMAND2 unveils the limitations of existing anomaly detection systems, more specifically the assumption of the authenticity of SCADA-received movement data, to which we propose mitigations for.
关键词： Industrial robots Vulnerability analysis Deception attacks Defenses
WU Xuelei, CHEN Lunqiang, PENG Yongzhen, WANG Pu, WANG Yayi
《环境科学与工程前沿（英文）》 2008年 第2卷 第2期 页码 236-240 doi: 10.1007/s11783-008-0008-x
基于拓扑绝缘体Sb2Te3的52 nm宽谱可调谐被动调Q掺镱光纤激光器 Research
王涛1,俞强1,2,郭琨1,史鑫尧2,阚雪芬3,徐轶君4,5,吴坚1,4,张凯2,周朴1
《信息与电子工程前沿（英文）》 2021年 第22卷 第3期 页码 287-436 doi: 10.1631/FITEE.2000577
标题 作者 时间 类型 操作
Insight into the role of cholesterol in modulation of morphology and mechanical properties of CHO-K1
Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen
期刊论文
China ADS sub-critical experimental assembly—Venus-1 and preliminary experiment
SHI Yongqian, XIA Pu, LUO Zhanglin, ZHAO Zhixiang, DING Dazhao, ZHU Qinfu, XIA Haihong, LI Jigen, ZHANG
期刊论文
Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with
null
期刊论文
Characteristics of two transferable aminoglycoside resistance plasmids in
Chengjun Pu, Xiaoyan Gong, Ying Sun
期刊论文
Game theoretic analysis of environmental impact assessment system in China
CHENG Hongguang, PU Xiao, GONG Li, QI Ye
期刊论文
New Challenges, New Opportunities and New Measures for Oil and Gas Cooperation in the Belt and Road
Yu-pu Wang,He Liu
期刊论文
Experimental study of nitrite accumulation in pre-denitrification biological nitrogen removal process
WU Xuelei, CHEN Lunqiang, PENG Yongzhen, WANG Pu, WANG Yayi
期刊论文
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