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Genome-wide search for candidate genes determining vertebrae number in pigs

Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG

《农业科学与工程前沿（英文）》 2017年第4卷第3期页码 327-334 doi: 10.15302/J-FASE-2017163

摘要： Longer porcine carcasses may be expected to have more vertebrae. Therefore, vertebrae number in pigs is an economically important trait. To examine the genetic basis of this trait, we genotyped 578 F Large White × Minzhu pigs using the Porcine SNP60K BeadChip. A genome-wide association study (GWAS) identified 36 significant single nucleotide polymorphisms (SNPs) on the chromosomes SSC1 (294.28–300.32 Mb) and SSC7 (102.22–109.39 Mb). A 6.04-Mb region that contained all 13 significant SNPs on SSC1 also contained the gene , previously reported to influence the number of vertebrae in pigs. However, the reported putative casual mutation of c.748C>T showed no genome-wide significant association with the trait, suggesting it was not a causal mutation in our population. The remaining 23 significant SNPs on SSC7 were concentrated in a 7.17-Mb region, which was within a quantitative trait locus interval for number of vertebrae. was the closest gene to the most significant SNP and might be a candidate. Haplotype sharing and block analysis refined the QTL to an interval of about 3 Mb containing 29 candidate genes. Of these 29 genes, the previously reported possible casual mutation of g.19034A>C was not found to be a causal mutation in our population. Exploration of these genes via additional genetic and functional studies in mammals revealed that could be a good candidate on SSC7. A mutation of c.1749G>A was detected by GWAS and could be proposed as a candidate causal mutation, or as closely linked to a causal mutation, for the number of vertebrae in pigs.

关键词： genome-wide association study number of vertebrae pig SSC7 TGFβ3

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中国工程科学2005年第7卷总目次

null

《中国工程科学》 2005年第7卷第12期页码 98-102

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Acid Orange 7 degradation using methane as the sole carbon source and electron donor

《环境科学与工程前沿（英文）》 2022年第16卷第3期 doi: 10.1007/s11783-021-1468-5

摘要：

• AO7 degradation was coupled with anaerobic methane oxidation.

关键词： Azo dyes AO7 degradation Anaerobic methane oxidation Microbial community ANME-2d

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Time-dependent metabolomics uncover dynamic metabolic adaptions in MCF-7 cells exposed to bisphenol A

《环境科学与工程前沿（英文）》 2023年第17卷第1期 doi: 10.1007/s11783-023-1604-5

摘要：

● Metabolomic temporal profiling of cells exposed to xenobiotics.

关键词： Metabolomics BPA MCF-7 Temporal profiling Metabolic adaption Dysregulation correlation

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Nicotinic acetylcholine receptor α7 subunit: a novel therapeutic target for cardiovascular diseases

null

《医学前沿（英文）》 2012年第6卷第1期页码 35-40 doi: 10.1007/s11684-012-0171-0

摘要：

Inflammation is important in the pathogenesis and development of cardiovascular diseases. Recent studies show that vagus nerve stimulation inhibits pro-inflammatory cytokine production through “the cholinergic anti-inflammatory pathway,” more specifically via the α7 nicotinic acetylcholine receptor (α7nAChR). In the current study, the role of the cholinergic anti-inflammatory pathway during septic shock, hypertension, and myocardial infarction is reviewed, and its possible clinical implications in cardiovascular diseases are discussed.

关键词： α7 nicotinic acetylcholine receptor cardiovascular diseases baroreflex sensitivity

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Resistance of large deformation of the Wushaoling Tunnel F7 soft fault

ZHAO Xufeng, CHEN Haiming, WANG Chunmiao

《结构与土木工程前沿（英文）》 2007年第1卷第1期页码 123-127 doi: 10.1007/s11709-007-0013-3

摘要： In this article, the mechanical characteristics of the squeeze of the F7 fault of the Wushaoling Tunnel are analyzed. The measurements and techniques are proposed to resist the deformation. The result indicates that the method of construction to control the further squeeze deflection is appropriate.

关键词： F7 deformation appropriate construction mechanical

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Research progress on human infection with avian influenza H7N9

Xiaoxin Wu, Lanlan Xiao, Lanjuan Li

《医学前沿（英文）》 2020年第14卷第1期页码 8-20 doi: 10.1007/s11684-020-0739-z

摘要： Since the first case of novel H7N9 infection was reported, China has experienced five epidemics of H7N9. During the fifth wave, a highly pathogenic H7N9 strain emerged. Meanwhile, the H7N9 virus continues to accumulate mutations, and its affinity for the human respiratory epithelial sialic acid 2-6 receptor has increased. Therefore, a pandemic is still possible. In the past 6 years, we have accumulated rich experience in dealing with H7N9, especially in terms of virus tracing, epidemiological research, key site mutation monitoring, critical disease mechanisms, clinical treatment, and vaccine development. In the research fields above, significant progress has been made to effectively control the spread of the epidemic and reduce the fatality rate. To fully document the research progress concerning H7N9, we reviewed the clinical and epidemiological characteristics of H7N9, the key gene mutations of the virus, and H7N9 vaccine, thus providing a scientific basis for further monitoring and prevention of H7N9 influenza epidemics.

关键词： H7N9 pandemic epidemiology mutations vaccine influenza

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A study on rapid acid chrome black (MB 7) spectrophotometric determination of ClO

Jing DONG, Huilong WANG

《化学科学与工程前沿（英文）》 2011年第5卷第2期页码 245-251 doi: 10.1007/s11705-010-1003-x

摘要： Experiments were conducted to investigate the degradation of 2,6-dinitro- -cresol (DNPC) in the chlorine dioxide (ClO ) catalytic oxidation process. Pure aluminum oxide was used as the catalyst in this process. The degradation of DNPC by ClO using aluminum oxide as catalyst was systematically studied by varying the experimental parameters, such as pH values, catalyst dosage, the initial concentration of DNPC and ClO , reaction time, etc. Under optimal condition (DNPC concentration 39 mg·L , ClO concentration 0.234 g·L , reaction time 15 min, catalyst dosage 4.7 g·L and pH 4.32), almost complete degradation of DNPC can be achieved. The kinetic studies revealed that the ClO catalytic oxidation degradation of DNPC followed pseudo-first-order kinetics with respect to both ClO and DNPC concentration. The repetitive use of the catalyst was investigated along sequential feed-batch trials. The catalyst performed efficiently after five runs. In addition, a simple and convenient method for the determination of ClO in water was developed by using acid chrome black 7 (MB 7) spectrophotometry in this paper.

关键词： chlorine dioxide 2 6-dinitro-p-cresol (DNPC) aluminum oxide water treatment MB 7 spectrophotometry

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RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

《医学前沿（英文）》页码 143-155 doi: 10.1007/s11684-022-0929-y

摘要： Gliomas are the most common central nervous system tumours; they are highly aggressive and have a poor prognosis. RGS16 belongs to the regulator of G-protein signalling (RGS) protein family, which plays an important role in promoting various cancers, such as breast cancer, pancreatic cancer, and colorectal cancer. Moreover, previous studies confirmed that let-7c-5p, a well-known microRNA, can act as a tumour suppressor to regulate the progression of various tumours by inhibiting the expression of its target genes. However, whether RGS16 can promote the progression of glioma and whether it is regulated by miR let-7c-5p are still unknown. Here, we confirmed that RGS16 is upregulated in glioma tissues and that high expression of RGS16 is associated with poor survival. Ectopic deletion of RGS16 significantly suppressed glioma cell proliferation and migration both in vitro and in vivo. Moreover, RGS16 was validated as a direct target gene of miR let-7c-5p. The overexpression of miR let-7c-5p obviously downregulated the expression of RGS16, and knocking down miR let-7c-5p had the opposite effect. Thus, we suggest that the suppression of RGS16 by miR let-7c-5p can promote glioma progression and may serve as a potential prognostic biomarker and therapeutic target in glioma.

关键词： RGS16 let-7c-5p glioma proliferation migration

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Erratum to “After Highflyer Crashes, Airborne Wind Energy Regroups” [Engineering 7 (2021) 277–279]

Peter Weiss

《工程（英文）》 2021年第7卷第10期页码 1506-1506 doi: 10.1016/j.eng.2021.08.004

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Universal influenza virus vaccines: what can we learn from the human immune response following exposure to H7

null

《医学前沿（英文）》 2017年第11卷第4期页码 471-479 doi: 10.1007/s11684-017-0602-z

摘要：

Several universal influenza virus vaccine candidates based on eliciting antibodies against the hemagglutinin stalk domain are in development. Typically, these vaccines induce responses that target group 1 or group 2 hemagglutinins with little to no cross-group reactivity and protection. Similarly, the majority of human anti-stalk monoclonal antibodies that have been isolated are directed against group 1 or group 2 hemagglutinins with very few that bind to hemagglutinins of both groups. Here we review what is known about the human humoral immune response to vaccination and infection with H7 subtype influenza viruses on a polyclonal and monoclonal level. It seems that unlike vaccination with H5 hemagglutinin, which induces antibody responses mostly restricted to the group 1 stalk domain, H7 exposure induces both group 2 and cross-group antibody responses. A better understanding of this phenomenon and the underlying mechanisms might help to develop future universal influenza virus vaccine candidates.

关键词： universal influenza virus vaccine hemagglutinin stalk H7N9

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Compiling of comprehensive data of human infections with novel influenza A (H7N9) virus

null

《医学前沿（英文）》 2013年第7卷第3期页码 275-276 doi: 10.1007/s11684-013-0285-z

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OVEREXPRESSION OF PTRLEA7, A LATE EMBRYOGENESIS ABUNDANT FAMILY GENE FROM PONCIRUS TRIFOLIATA, CONFERS

《农业科学与工程前沿（英文）》 2021年第8卷第2期

摘要：

Late embryogenesis abundant (LEA) genes encode highly hydrophilic proteins that are essential in abiotic stress responses. However, most LEA genes in higher plants have not yet been investigated. This study identified an LEA family gene (PtrLEA7) from Poncirus trifoliata and studied its function in drought tolerance. The full-length coding sequence of PtrLEA7 was 420 bp encoding a protein of 139 amino acids. Phylogenetic analysis shows that PtrLEA7 protein belongs to the LEA_4 subfamily. Expression profiling by qPCR found that PtrLEA7 was strongly induced by dehydration, cold and ABA treatments, and slightly induced by salt stress. Subcellular localization reveals that PtrLEA7 protein was located in both cytoplasm and nucleus. To investigate its function, transgenic plants of both tobacco and Poncirus trifoliata overexpressing PtrLEA7 were obtained. Stress tolerance assays show that overexpression lines had enhanced dehydration and drought tolerance compared with wild type plants, indicating that PtrLEA7 positively regulates drought tolerance. In addition, transgenic plants had much higher expression levels of three antioxidant enzyme genes (CAT, SOD and POD) and significantly increased catalase enzyme activity, accompanied by reduced reactive oxygen species accumulation in comparison with wild type plants. Collectively, this study demonstrates that PtrLEA7 can confer enhanced drought tolerance partially via enhancing antioxidant capacity.

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基于细胞打印的三维MCF-7乳腺癌细胞球高通量构建研究 Article

凌楷,黄国友,刘俊聪,张晓慧,马玉菲,卢天健,徐峰

《工程（英文）》 2015年第1卷第2期页码 269-274 doi: 10.15302/J-ENG-2015062

摘要：

基于细胞球的体外组织模型在生理病理机理和药物筛选方面具有重要的研究意义和广阔的应用前景，利用微纳生物制造技术可控构建细胞球已吸引了越来越多研究者的关注。微孔板法作为一种简单易行的细胞球构建方法经常被研究者采用，但目前的微孔板法存在结构制备和可控性不足、细胞种植不均匀以及细胞容易流失等问题。本文利用自行搭建的细胞打印技术制备载细胞的明胶微凝胶作为模板，构建了带有凹面微孔阵列的聚乙二醇二甲基丙烯酸酯水凝胶微孔板芯片并原位形成了乳腺癌细胞球。本方法操作灵活、可控性好，避免了细胞种植不均匀和细胞流失等问题，在组织工程、再生医学以及药物筛选等研究具有重要的应用价值。

关键词： MCF-7细胞球细胞打印水凝胶微孔板芯片

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Precise semi-analytical inverse kinematic solution for 7-DOF offset manipulator with arm angle optimization

《机械工程前沿（英文）》 2021年第16卷第3期页码 435-450 doi: 10.1007/s11465-021-0630-x

摘要： Seven-degree-of-freedom redundant manipulators with link offset have many advantages, including obvious geometric significance and suitability for configu-ration control. Their configuration is similar to that of the experimental module manipulator (EMM) in the Chinese Space Station Remote Manipulator System. However, finding the analytical solution of an EMM on the basis of arm angle parameterization is difficult. This study proposes a high-precision, semi-analytical inverse method for EMMs. Firstly, the analytical inverse kinematic solution is established based on joint angle parameterization. Secondly, the analytical inverse kinematic solution for a non-offset spherical–roll–spherical (SRS) redundant manipulator is derived based on arm angle parameterization. The approximate solution of the EMM is calculated in accordance with the relationship between the joint angles of the EMM and the SRS manipulator. Thirdly, the error is corrected using a numerical method through the analytical inverse solution based on joint angle parameterization. After selecting the stride and termination condition, the precise inverse solution is computed for the EMM based on arm angle parameterization. Lastly, case solutions confirm that this method has high precision, and the arm angle parameterization method is superior to the joint angle parameterization method in terms of parameter selection.

关键词： 7-DOF redundant manipulator inverse kinematics semi-analytical arm angle link offset

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标题作者时间类型操作

Genome-wide search for candidate genes determining vertebrae number in pigs

Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG

期刊论文

中国工程科学2005年第7卷总目次

null

期刊论文

Acid Orange 7 degradation using methane as the sole carbon source and electron donor

期刊论文

Time-dependent metabolomics uncover dynamic metabolic adaptions in MCF-7 cells exposed to bisphenol A

期刊论文

Nicotinic acetylcholine receptor α7 subunit: a novel therapeutic target for cardiovascular diseases

null

期刊论文

Resistance of large deformation of the Wushaoling Tunnel F7 soft fault

ZHAO Xufeng, CHEN Haiming, WANG Chunmiao

期刊论文

Research progress on human infection with avian influenza H7N9

Xiaoxin Wu, Lanlan Xiao, Lanjuan Li

期刊论文

A study on rapid acid chrome black (MB 7) spectrophotometric determination of ClO

Jing DONG, Huilong WANG

期刊论文

RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

期刊论文

Erratum to “After Highflyer Crashes, Airborne Wind Energy Regroups” [Engineering 7 (2021) 277–279]

Peter Weiss

期刊论文

Universal influenza virus vaccines: what can we learn from the human immune response following exposure to H7

null

期刊论文

Compiling of comprehensive data of human infections with novel influenza A (H7N9) virus

null

期刊论文

OVEREXPRESSION OF PTRLEA7, A LATE EMBRYOGENESIS ABUNDANT FAMILY GENE FROM PONCIRUS TRIFOLIATA, CONFERS

期刊论文

基于细胞打印的三维MCF-7乳腺癌细胞球高通量构建研究

凌楷,黄国友,刘俊聪,张晓慧,马玉菲,卢天健,徐峰

期刊论文

Precise semi-analytical inverse kinematic solution for 7-DOF offset manipulator with arm angle optimization

期刊论文

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