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Bone regeneration by stem cell and tissue engineering in oral and maxillofacial region

Zhiyuan Zhang

《医学前沿（英文）》 2011年第5卷第4期页码 401-413 doi: 10.1007/s11684-011-0161-7

摘要： Clinical imperatives for the reconstruction of jaw bone defects or resorbed alveolar ridge require new therapies or procedures instead of autologous/allogeneic bone grafts. Regenerative medicine, based on stem cell science and tissue engineering technology, is considered as an ideal alternative strategy for bone regeneration. In this paper, we review the current choices of cell source and strategies on directing the osteogenic differentiation of stem cells. The preclinical animal models for bone regeneration and the key translational points to clinical success in oral and maxillofacial region are also discussed. We propose comprehensive strategies based on stem cell and tissue engineering researches, allowing for clinical application in oral and maxillofacial region.

关键词： bone regeneration animal models translational strategies oral and maxillofacial region

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Overcoming the challenges of conducting translational research in cell therapy

Sowmya Viswanathan, Armand Keating

《医学前沿（英文）》 2011年第5卷第4期页码 333-335 doi: 10.1007/s11684-011-0166-2

摘要： Cell therapy holds promise in addressing a number of unmet medical needs but despite its considerable impetus, faces numerous challenges including support for translational research in this area. Here, we examine the challenges confronting cell therapy research including the limited funding available for translational research partly due to the uncompetitive nature of required validation studies in cell therapy that of necessity are not hypothesis-driven. Other issues include lack of standardization of definitions of cell products, uncertainty regarding optimum preclinical animal models, duplication of efforts to secure regulatory approval and a lack of uniformity of nomenclature for some cell products. Nonetheless, several initiatives to address all of these issues are underway.

关键词： stem cells clinical trials funding animal models standardization common definitions

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Molecular mechanisms and therapeutic strategies of vulnerable atherosclerotic plaques

Wen-Qiang CHEN MD, Yun ZHANG MD, PhD, FACC,

《医学前沿（英文）》 2010年第4卷第1期页码 36-42 doi: 10.1007/s11684-010-0020-y

摘要： Vulnerable atherosclerotic plaque rupture leading to thrombosis is the major cause of acute coronary syndrome (ACS). Studies on the pathophysiologic mechanism of both ACS and plaque stabilizing treatment are driving the development of animal models of vulnerable plaque. In our laboratory, we established animal models of plaque rupture and thrombosis in rabbits and mice that are similar to human plaque rupture. Potential mechanisms involved in plaque vulnerability were studied from the inflammation-immunity, proliferation-apoptosis, oxidative stress and biomechanics aspects. Imaging markers and biomarkers were used to detect vulnerable plaques, including high frequency duplex ultrasound, intravascular ultrasound (IVUS), intravascular ultrasound elastography, magnetic resonance imaging (MRI) and inflammatory markers. Effective gene and drug strategies to treat vulnerable plaques were explored.

关键词： vulnerable plaque animal models mechani-sm detection treatment

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Multiple effects of grape seed polyphenolics to prevent metabolic diseases

Torey ARVIK, Hyunsook KIM, James SEIBER, Wallace YOKOYAMA

《农业科学与工程前沿（英文）》 2018年第5卷第3期页码 351-361 doi: 10.15302/J-FASE-2018235

摘要：

Obesity is increasing in developing countries. Population studies show a relationship between affluence and obesity. Changing food intake patterns with affluence such as preference for foods with less astringent polyphenolic compounds and dietary fibers may increase risk of metabolic dysfunctions due to caloric imbalance. Animal models of obesity consistently show that grape seed procyanidins prevent increases in body and abdo- minal adipose weight gain, plasma cholesterol, liver weight gain and inflammation in animals on high fat diets. The mechanisms are not clear because the oral intake of procyanidins results in pleiotropic interactions with proteins in the mouth, stomach, small intestine, cecum and colon that affect the rate of digestion of bioavailability of macronutrients, sterols, and dietary fiber. Procyanidins also bind bile acids and reduce intestinal permeability to inflammatory bacterial cell wall fragment. Procyanidins are not degraded or metabolized until reaching the lower gut where they can be metabolized into phenolic acids by gut bacteria. While they are metabolized by gut bacteria, they also alter total numbers and distribution of phyla and species of gut bacteria. Gut bacteria are recognized as significant contributors to obesity and obesity related metabolic diseases. The review examines the different pleiotropic effects of grape seed procyanidins that have a significant effect on metabolic disease in animal models of obesity.

关键词： grape seed obesity procyanidins high fat microbiota animal models

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Highlights for special issue on “Large Animal Stem Cells”

Jianyong HAN

《农业科学与工程前沿（英文）》 2019年第6卷第1期页码 1-2 doi: 10.15302/J-FASE-2019251

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Cryopreservation of farm animal gametes and embryos: recent updates and progress

Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU

《农业科学与工程前沿（英文）》 2019年第6卷第1期页码 42-53 doi: 10.15302/J-FASE-2018231

摘要：

Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously refined over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been significant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.

关键词： vitrification gametes embryo animal production cryoprotective agent freezability

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A brief review of genome editing technology for generating animal models

Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH

《农业科学与工程前沿（英文）》 2020年第7卷第2期页码 123-128 doi: 10.15302/J-FASE-2019309

摘要：

The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species.

关键词： animal model CRISPR genome editing TALEN ZFN

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Highlights of special issue on Animal Science

Jun REN

《农业科学与工程前沿（英文）》 2017年第4卷第3期页码 257-259 doi: 10.15302/J-FASE-2017165

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Antimicrobial use in food animal production: situation analysis and contributing factors

Ziping WU

《农业科学与工程前沿（英文）》 2018年第5卷第3期页码 301-311 doi: 10.15302/J-FASE-2018207

摘要：

By measuring the quantity and ways in which antimicrobials are used, and reviewing different technical and socioeconomic factors influencing antimicrobial use at farm level, this study discusses the main knowledge gaps in antimicrobial use in food animal production and provides recommendations for future research and policy development. The review reveals that antimicrobial use in food animals exhibit strong regional and species differences, and there are still large information gaps concerning the current state of antimicrobial use. Factors associated with animal health (including antimicrobial resistance), animal health improvement, economic costs and benefits relevant to animal diseases, and potential technological alternatives or alternative systems all have an impact on antimicrobial use on the farm. There is a clear need to resolve the data gap by monitoring antimicrobial use and developing an analytical framework to better understand farmer behaviors under different technical, economic and environmental circumstances.

关键词： antimicrobial use drivers for antimicrobial use food animal production

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The speciation, leachability and bioaccessibility of Cu and Zn in animal manure-derived biochar: effect

Qi Lin, Xin Xu, Lihua, Wang, Qian Chen, Jing Fang, Xiaodong Shen, Liping Lou, Guangming Tian

《环境科学与工程前沿（英文）》 2017年第11卷第3期 doi: 10.1007/s11783-017-0924-8

摘要： Biochars derived from animal manures may accumulate potentially toxic metals and cause a potential risk to ecosystem. The synchrotron-based X-ray spectroscopy, sequential fractionation schemes, bioaccessibility extraction and leaching procedure were performed on poultry and swine manure-derived biochars (denoted PB and SB, respectively) to evaluate the variance of speciation and activity of Cu and Zn as affected by the feedstock and pyrolysis temperature. The results showed that Cu speciation was dependent on the feedstock with Cu-citrate-like in swine manure and species resembling Cu-glutathione and CuO in poultry manure. Pyrolyzed products, however, had similar Cu speciation mainly with species resembling Cu-citrate, CuO and CuS/Cu S. Organic bound Zn and Zn (PO ) -like species were dominant in both feedstock and biochars. Both Cu and Zn leaching with synthetic precipitation leaching procedure (SPLP) and toxicity characteristic leaching procedure (TCLP) decreased greatly with the rise of pyrolysis temperature, which were consistent with the sequential extraction results that pyrolysis converted Cu and Zn into less labile phases such as organic/sulfide and residual fractions. The potential bioaccessibility of Zn decreased for both the PB and SB, closely depending on the content of non-residual Zn. The bioaccessibility of Cu, however, increased for the SB prepared at 300°C–700°C, probably due to the increased proportion of CuO. Concerning the results of sequential fractionation schemes, bioaccessibility extraction and leaching procedure, pyrolysis at 500°C was suggested as means of reducing Cu/Zn lability and poultry manure was more suitable for pyrolysis treatment.

关键词： Animal manure Biochar Metals Molecular species Pyrolysis

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Electrolyzed water and its application in animal houses

Weichao ZHENG,Li NI,Baoming LI

《农业科学与工程前沿（英文）》 2016年第3卷第3期页码 195-205 doi: 10.15302/J-FASE-2016109

摘要： Electrolyzed water (EW) can be produced by electrolysis of a dilute salt solution. Slightly acidic electrolyzed water (SAEW, pH 5.0–6.5) and neutral electrolyzed water (NEW, pH 6.5–8.5) are considered healthy and environmentally friendly because no hazardous chemicals are added in its production, there is reduced corrosion of surfaces and it minimizes the potential for damage to animal and human health. Over the last decade, EW has become increasingly popular as an alternative disinfectant for decontamination in animal houses. However, there have been some issues related to EW that are not well known, including different mechanisms for generation of SAEW and NEW, and the antimicrobial mechanism of EW. This review covers the definitions of SAEW and NEW, different generation systems for SAEW and NEW, the antimicrobial mecha- nism of EW, and recent developments related to the application of SAEW and NEW in animal houses.

关键词： disinfection poultry and livestock slightly acidic electrolyzed water neutral electrolyzed water

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Animal stem cells: the engineering development front of 2018

Wenye YUAN, Jianxiang XU, Chenying LI, Lijuan SHI, Aidong YANG, Yan WANG, Lu XING, Shaozhong KANG

《农业科学与工程前沿（英文）》 2019年第6卷第1期页码 93-95 doi: 10.15302/J-FASE-2018250

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极地动物基因资源研究现状与发展战略

陈松林,徐文腾,陈张帆

《中国工程科学》 2019年第21卷第6期页码 39-47 doi: 10.15302/J-SSCAE-2019.06.007

摘要：

本文主要从基因组和转录组层面分析了极地动物基因资源的研究现状，梳理了本领域研究中存在的问题，并提出了未来发展战略。极地动物基因组测序起步较晚，迄今只完成了 13种极地动物的全基因组测序。在转录组研究方面，人们对极地的 31个物种进行了转录组测序，并在以下四个方向重点开展了研究：环境适应性研究；污染物应激反应的分子机制研究；不同发育阶段或不同组织中的转录组分析；功能基因挖掘。本领域研究由于起步晚，研究广度和深度都有待加强，但极地动物的基因资源研究具有战略意义。建议国家设立“极地动物基因资源发掘与应用”重点研发计划专项，围绕极地渔业动物特殊性状遗传解析、特有基因功能分析和基因工程产品研发等开展研究。

关键词：极地动物基因组，转录组，基因资源

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Comparison of analytical procedures for measuring phosphorus content of animal manures in China

null

《农业科学与工程前沿（英文）》 2019年第6卷第4期页码 431-440 doi: 10.15302/J-FASE-2019279

摘要：

The concentration and components of manure phosphorus (P) are key factors determining potential P bioavailability and runoff. The distribution of P forms in swine, poultry and cattle manures collected from intensive and extensive production systems in several areas of China was investigated with sequential fractionation and a simplified two-step (NaHCO -NaOH/EDTA) procedures. The mean total P concentration, determined by the sequential fractionation procedure of intensive swine, poultry and cattle manure, expressed as g·kg , was 14.9, 13.4 and 5.8 g·kg , respectively, and 4.4 g·kg in extensive cattle manure. In intensive swine, poultry and cattle manure about 73%, 74% and 79% of total P, respectively, was bioavailable (i.e., P extracted by H O and NaHCO ) and 78% in extensive cattle manure. The results indicated the relative environmental risk, from high to low, of swine, poultry and cattle manure. There is considerable regional variation in animal manure P across China, which needs to be considered when developing manure management strategies.

关键词： diet phosphorus manure phosphorus sequential P fractionation

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A review of traditional and novel detection techniques for melamine and its analogues in foods and animal

Mengshi LIN,

《化学科学与工程前沿（英文）》 2009年第3卷第4期页码 427-435 doi: 10.1007/s11705-009-0244-z

摘要： Melamine, a nitrogen-rich chemical, has received much attention in recent years due to a series of highly publicized food safety incidents, including 2007 pet food recalls in North America and 2008 melamine contamination in milk, infant formula, and other milk-derived products in China. Current analytical methods for testing melamine are time-consuming, expensive, and labor-intensive. Therefore, there is an increasing interest in the food science and analytical chemistry field to develop simple, quick, sensitive, and cost-effective methods for detection of melamine and its analogues (e.g., cyanuric acid, melamine cyanurate, ammelide, and ammeline) in food ingredients, processed food, and animal feed. This review aims to summarize traditional and novel analytical techniques that have been used or show great potential to detect, characterize, and quantify melamine and its analogues in foods and animal feed.

关键词： milk-derived Melamine labor-intensive cyanurate time-consuming